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The effect of human immune IgG on the in vitro development of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

G. H. Mitchell ,
G. A. Butcher ,
A. Voller  and
S. Cohen
G. H. Mitchell
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
G. A. Butcher
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
A. Voller
Affiliation:
Nuffield Institute of Comparative Medicine, Zoological Society of London, Regent's Park, London, N.W.I, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
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Extract

Plasmodium falciparum parasites infecting Aotus trivirgatus erythrocytes were cultured in media (Harvard and TC199) augmented with human, foetal calf, or other sera. Conditions were established which supported growth of parasites and allowed cyclical multiplication when fresh erythrocytes (from Aotus or Homo) were added in sub-culture (mean multiplication rate: x 3). Immunoglobulin G pools, prepared from plasma collected in endemic malarious areas in Africa and from unexposed Britons, were tested for effects on the in vitro growth (measured by incorporation of tritiated leucine) and multiplication of parasites. Whilst non-immune IgG was without effect, IgG from both East and West Africa inhibited the multiplication of East African (Uganda-Palo Alto strain) parasites.

Type
Research Article
Information
Parasitology , Volume 72 , Issue 2 , April 1976 , pp. 149 - 162
Copyright
Copyright © Cambridge University Press 1976

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