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The effect of glycosylation of antigens on the antibody responses against Echinostoma caproni (Trematoda: Echinostomatidae)

Published online by Cambridge University Press:  14 May 2014

JAVIER SOTILLO ,
ALBA CORTÉS ,
CARLA MUÑOZ-ANTOLI ,
BERNARD FRIED ,
J. GUILLERMO ESTEBAN  and
RAFAEL TOLEDO
JAVIER SOTILLO*
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot – Valencia, Spain
ALBA CORTÉS
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot – Valencia, Spain
CARLA MUÑOZ-ANTOLI
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot – Valencia, Spain
BERNARD FRIED
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
J. GUILLERMO ESTEBAN
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot – Valencia, Spain
RAFAEL TOLEDO
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot – Valencia, Spain
*
*Corresponding author: Present address: Centre for Biodiscovery and Molecular Development of Therapeutics, Building E4, James Cook University, McGregor Rd, Smithfield, QLD 4878, Australia. E-mail: [email protected]
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Summary

In the present study, we analyse the effect of glycosylation in Echinostoma caproni (Trematoda: Echinostomatidae) antigens in antibody responses against the parasite in experimentally infected mice. It has been previously demonstrated that the mouse is a host of high compatibility with E. caproni and develops elevated responses of IgG, IgG1, IgG3 and IgM as a consequence of the infection, though the role of glycans in these responses remains unknown. To this purpose, the responses generated in mice against non-treated excretory/secretory antigens of E. caproni were compared with those observed after N-deglycosylation, O-deglycosylation and double deglycosylation of the antigens by indirect ELISA and western blot. Our results suggest that E. caproni-expressed glycans play a major role in the modulation of the immune responses. The results obtained indicate that IgG subclass responses generated in mice against E. caproni are essentially due to glycoproteins and may affect the Th1/Th2 biasing. The reactivity significantly decreased after any of the deglycosylation treatments and the N-glycans appears to be of greater importance than O-glycans. Interestingly, the IgM response increased after N-deglycosylation suggesting that carbohydrates may mask peptide antigens.

Keywords

TrematodaEchinostoma capronimousedeglycosylationglycoproteincarbohydratesimmunoglobulinwestern blot
Type
Research Article
Information
Parasitology , Volume 141 , Issue 10 , September 2014 , pp. 1333 - 1340
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Copyright
Copyright © Cambridge University Press 2014 

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