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Effect of bioactive compounds from Sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides

Published online by Cambridge University Press:  16 June 2005

E. BARRAU ,
N. FABRE ,
I. FOURASTE  and
H. HOSTE
E. BARRAU
Affiliation:
Unité Mixte de Recherches 152, IRD/UPS “Pharmacochimie des substances naturelles et pharmacophores redox” Faculté des Sciences Pharmaceutiques, Université Toulouse III, 35 Chemin des Maraîchers, F31062 Toulouse Cedex 4, France
N. FABRE
Affiliation:
Unité Mixte de Recherches 152, IRD/UPS “Pharmacochimie des substances naturelles et pharmacophores redox” Faculté des Sciences Pharmaceutiques, Université Toulouse III, 35 Chemin des Maraîchers, F31062 Toulouse Cedex 4, France
I. FOURASTE
Affiliation:
Unité Mixte de Recherches 152, IRD/UPS “Pharmacochimie des substances naturelles et pharmacophores redox” Faculté des Sciences Pharmaceutiques, Université Toulouse III, 35 Chemin des Maraîchers, F31062 Toulouse Cedex 4, France
H. HOSTE
Affiliation:
Unité Mixte de Recherches 1225 INRA/DGER, “Interactions Hôte Agents Pathogènes”, École Nationale Vétérinaire de Toulouse, 23 chemin des Capelles, F31076 Toulouse, France
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Abstract

Anthelmintic bioactivity against gastrointestinal nematodes has been associated with leguminous forages supporting the hypothesis of a role of condensed tannins. However, the possibility that other compounds might also been involved has received less consideration. Using bio-guided fractionation, the current study aimed at characterizing the biochemical nature of the active compounds present in sainfoin (Onobrychis viciifolia), previously identified as an anthelmintic leguminous forage. The effects of sainfoin extracts were evaluated on 3rd-stage larvae (L3) of Haemonchus contortus by using a larval migration inhibition (LMI) assay. Comparison of extracts obtained with several solvent systems showed that the bioactivity was associated with the 70[ratio ]30 acetone/water extract. Further fractionation of the later allowed the separation of phenolic compounds. By use of a dialysis method, compounds were separated with a molecular weight cut-off of 2000 Da. The in vitro anthelmintic effect of the fraction with condensed tannins was confirmed. In the fraction containing molecules of MW <2000 Da, 3 flavonol glycosides were identified as rutin, nicotiflorin and narcissin. At 1200 μg/ml, each inhibited significantly the migration of larvae. Addition of polyvinyl pyrrolidone (PVPP) to both fractions before incubation restored larval migration. These results confirmed the role of both tannins and flavonol glycosides in the anthelmintic properties of sainfoin.

Keywords

sainfoin (Onobrychis viciifolia Scop.)bio-guided fractionationHaemonchus contortustanninsflavonol glycosides
Type
Research Article
Information
Parasitology , Volume 131 , Issue 4 , October 2005 , pp. 531 - 538
Copyright
© 2005 Cambridge University Press

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