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Echinococcus multilocularis in a Eurasian lynx (Lynx lynx) in Turkey

Published online by Cambridge University Press:  07 February 2018

Hamza Avcioglu ,
Esin Guven ,
Ibrahim Balkaya  and
Ridvan Kirman
Hamza Avcioglu
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Esin Guven*
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Ibrahim Balkaya
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Ridvan Kirman
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
*
Author for correspondence: Esin Guven, E-mail: [email protected]
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Abstract

Echinococcus multilocularis is the causative agent of alveolar echinococcosis (AE), one of the most threatening zoonoses in Eurasia. Human AE is widespread in the Erzurum region of Turkey, but the situation of the disease in intermediate and definitive hosts is unknown. A Eurasian lynx (Lynx lynx) was killed in a traffic accident in the north of Erzurum, and was taken to our laboratory. Sedimentation and counting technique (SCT), DNA isolation and polymerase chain reaction (PCR) analysis were performed. The SCT results showed that the lynx was infected with E. multilocularis with a medium (745 worms) worm burden. The DNA of adult worms obtained from the lynx was analyzed with a species-specific PCR, and the worms were confirmed to be E. multilocularis by 12S rRNA gene sequence analysis. This is the first report of E. multilocularis from Eurasian lynx in Turkey.

Keywords

Echinococcus multilocularisEurasian lynxPCRTurkey
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1147 - 1150
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Copyright
Copyright © Cambridge University Press 2018 

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References

Altintas, N (2003) Past to present: Echinococcosis in Turkey. Acta Tropica 85, 105–112.Google Scholar
Avcioglu, H, Guven, E, Balkaya, I, Kirman, R, Bia, MM and Gulbeyen, H (2016) First molecular characterization of Echinococcus multilocularis in Turkey. Vector Borne and Zoonotic Diseases 16, 627–629.Google Scholar
Avcioglu, H, Guven, E, Balkaya, I, Kirman, R, Bia, MM, Gulbeyen, H, Kurt, A, Yaya, S and Demirtas, S (2017) First detection of Echinococcus multilocularis in rodent intermediate hosts in Turkey. Parasitology 144(13), 1821–1827.Google Scholar
Boufana, B, Umhang, G, Qiu, J, Chen, X, Lahmar, S, Boué, F, Jenkins, DJ and Craig, PS (2013) Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China. American Journal of Tropical Medicine and Hygiene 88, 795–802.Google Scholar
Carmena, D and Cardona, GA (2014) Echinococcosis in wild carnivorous species: Epidemiology, genotypic diversity, and implications for veterinary public health. Veterinary Parasitology 202, 69–94.Google Scholar
Chynoweth, MW, Çoban, E and Şekercioğlu, ÇH (2015) Conservation of a new breeding population of Caucasian lynx (Lynx lynx dinniki) in eastern Turkey. Turkish Journal of Zoology 39, 541–543.Google Scholar
Conraths, FJ and Deplazes, P (2015) Echinococcus multilocularis: Epidemiology, surveillance and state-of-the-art diagnostics from a veterinary public health perspective. Veterinary Parasitology 213, 149–161.Google Scholar
Deplazes, P, Dinkel, A and Mathis, A (2003) Molecular tools for studies on the transmission biology of Echinococcus multilocularis. Parasitology 127, 53–61.Google Scholar
Deplazes, P, Rinaldi, L, Alvarez Rojas, CA, Torgerson, PR, Harandi, MF, Romig, T, Antolova, D, Schurer, JM, Lahmar, S, Cringoli, G, Magambo, J, Thompson, RC and Jenkins, EJ (2017) Global distribution of alveolar and cystic echinococcosis. Advances in Parasitology 95, 315–493.Google Scholar
Duscher, G, Prosl, H and Joachim, A (2005) Scraping or shaking a comparison of methods for the quantitative determination of Echinococcus multilocularis in fox intestines. Parasitology Research 95, 40–42.Google Scholar
Dyachenko, V, Beck, E, Pantchev, N and Bauer, C (2008) Cost-effective method of DNA extraction from taeniid eggs. Parasitology Research 102, 811–813.Google Scholar
Eckert, J., Deplazes, P., Craig, P. S., Gemmell, M. A., Gottstein, B., Heath, D., Jenkins, D. J., Kamiya, M. and Lightowlers, M. (2001). Echinococcosis in animals: clinical aspects, diagnosis and treatment. In Eckert, J, Gemmell, MA, Meslin, FX and Pawlowski, ZS (eds). WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. Paris, France: WHO/OIE, pp. 72–99.Google Scholar
Eckert, J., Deplazes, P. and Kern, P. (2011). Alveolar echinococcosis (Echinococcus multilocularis) and neotropical forms of echinococcosis (Echinococcus vogeli and Echinococcus oligarthrus). In Brown, D, Palmer, S, Torgerson, PR and Soulsby, EJL (eds). Zoonoses, 2nd edn, Oxford: Oxford University Press, pp. 671–701.Google Scholar
Gottstein, B, Saucy, F, Deplazes, P, Reichen, J, Demierre, G, Busato, A, Zuercher, C and Pugin, P (2001) Is high prevalence of Echinococcus multilocularis in wild and domestic animals associated with disease incidence in humans? Emerging Infectious Diseases 7, 408–412.Google Scholar
Hegglin, D and Deplazes, P (2013) Control of Echinococcus multilocularis: strategies, feasibility and cost-benefit analyses. International Journal of Parasitology 43(5), 327–337.Google Scholar
Hegglin, D, Bontadina, F and Deplazes, P (2015) Human-wildlife interactions and zoonotic transmission of Echinococcus multilocularis. Trends in Parasitology 31, 167–173.Google Scholar
Hofer, S, Gloor, S, Müller, U, Mathis, A, Hegglin, D and Deplazes, P (2000) High prevalence of Echinococcus multilocularis in urban red foxes (Vulpes vulpes) and voles (Arvicola terrestris) in the city of Zurich, Switzerland. Parasitology 120, 135–142.Google Scholar
Jenkins, DJ and Romig, T (2000) Efficacy of Droncit® spot-on (praziquantel) 4% w/v against immature and mature Echinococcus multilocularis in cats. International Journal of Parasitology 30, 959–962.Google Scholar
Kapel, CMO, Torgerson, PR, Thompson, RCA and Deplazes, P (2006) Reproductive potential of Echinococcus multilocularis in experimentally infected foxes, dogs, raccoon dogs and cats. International Journal of Parasitology 36, 79–86.Google Scholar
Knapp, J, Umhang, G, Poulle, ML and Millona, L (2016a) Development of a real time PCR for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of Toxocara spp. and Echinococcus multilocularis. Applied and Environmental Microbiology 82(10), 2950–2958.Google Scholar
Knapp, J, Combes, B, Umhang, G, Aknouche, S and Millon, L (2016b) Could the domestic cat play a significant role in the transmission of Echinococcus multilocularis? A study based on qPCR analysis of cat feces in a rural area in France. Parasite 23, 42.Google Scholar
Merdivenci, A (1963) Türkiye'de tilki (Vulpes vulpes) lerde ilk helmintolojik araştırma ve ilk Echinococcus multilocularis (Leuckart, 1884) Vogel, 1935 olayı. Veteriner Hekimler Derneği Dergisi 3, 290.Google Scholar
Nonaka, N, Hirokawa, H, Inoue, T, Nakao, R, Ganzorig, S, Kobayashi, F, Inagaki, M, Egoshi, K, Kamiya, M and Oku, Y (2008) The first instance of a cat excreting Echinococcus multilocularis eggs in Japan. Parasitology International 57, 519–520.Google Scholar
Otranto, D, Cantacessi, C, Dantas-Torres, F, Brianti, E, Pfeffer, M, Genchi, C, Guberti, V, Capelli, G and Deplazes, P (2015) The role of wild canids and felids in spreading parasites to dogs and cats in Europe. Part II: Helminths and arthropods. Veterinary Parasitology 213, 24–37.Google Scholar
Pomamarev, N, Tikhaya, N, Kostykov, M and Nekrasov, V (2011) Helminth fauna of wild carnivores in different ecological zones of the Altay District. Bulletin of the Altay State Agrarian University 5, 64–67.Google Scholar
Romig, T, Deplazes, P, Jenkins, D, Giraudoux, P, Massolo, A, Craig, PS, Wassermann, M, Takahashi, K and de la Rue, M (2017) Ecology and life cycle patterns of Echinococcus species. Advances in Parasitology 95, 213–314.Google Scholar
Salinas-López, N, Jiménez-Guzmán, F and Cruz-Reyes, A (1996) Presence of Echinococcus oligarthrus (Diesing, 1863) Lühe, 1910 in Lynx rufus texensis Allen, 1895 from San Fernando, Tamaulipas state, in north-east Mexico. International Journal of Parasitology 26, 793–796.Google Scholar
Thompson, RCA (2013) Parasite zoonoses and wildlife: one health, spillover and human activity. International Journal of Parasitology 43, 1079–1088.Google Scholar
Thompson, RCA, Deplazes, P and Eckert, J (2003) Observations on the development of Echinococcus multilocularis in cats. Journal of Parasitology 89, 1086–1088.Google Scholar
Thompson, RC, Kapel, CM, Hobbs, RP and Deplazes, P (2006) Comparative development of Echinococcus multilocularis in its definitive hosts. Parasitology 132, 709–716.Google Scholar
Torgerson, PR, Keller, K, Magnotta, M and Ragland, N (2010) The global burden of alveolar Echinococcosis. PLoS Neglected Tropical Diseases 4, e722.Google Scholar
Trachsel, D, Deplazes, P and Mathis, A (2007) Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA. Parasitology 134, 911–920.Google Scholar
Umhang, G, Forin-Wiart, M, Hormaz, V, Caillot, C, Boucher, JM, Poulle, ML and Boué, F (2015) Echinococcus multilocularis detection in the intestines and feces of free-ranging domestic cats (Felis s. catus) and European wildcats (Felis s. silvestris) from northeastern France. Veterinary Parasitology 214, 75–79.Google Scholar
Umhang, G, Lahoreau, J, Hormaz, V, Boucher, JM, Guenon, A, Montange, D, Grenouillet, F and Boue, F (2016) Surveillance and management of Echinococcus multilocularis in a wildlife park. Parasitology International 65, 245–250.Google Scholar