Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-23T23:38:52.140Z Has data issue: false hasContentIssue false
  • English
  • Français

Echinococcus granulosus strain typing in North Africa: comparison of eight nuclear and mitochondrial DNA fragments

Published online by Cambridge University Press:  01 March 2004

J.-M. BART ,
K. BARDONNET ,
M. C. B. ELFEGOUN ,
H. DUMON ,
L. DIA ,
D. A. VUITTON  and
R. PIARROUX
J.-M. BART
Affiliation:
Santé Environnement Rural Franche-Comté and WHO Centre Collaborating for Prevention and Treatment of Human Echinococcosis, University of Franche-Comté, 25000 Besançon, France
K. BARDONNET
Affiliation:
Santé Environnement Rural Franche-Comté and WHO Centre Collaborating for Prevention and Treatment of Human Echinococcosis, University of Franche-Comté, 25000 Besançon, France
M. C. B. ELFEGOUN
Affiliation:
Laboratory of Parasitology, Veterinary Department, University of Mentouri, Constantine, Algeria
H. DUMON
Affiliation:
Parasitology and Mycology Department, ‘La Timone’ Hospital, 13 385 Marseille
L. DIA
Affiliation:
National Center for Veterinary Studies and Research (CNERV), Nouakchott, Mauritania
D. A. VUITTON
Affiliation:
Santé Environnement Rural Franche-Comté and WHO Centre Collaborating for Prevention and Treatment of Human Echinococcosis, University of Franche-Comté, 25000 Besançon, France
R. PIARROUX
Affiliation:
Santé Environnement Rural Franche-Comté and WHO Centre Collaborating for Prevention and Treatment of Human Echinococcosis, University of Franche-Comté, 25000 Besançon, France
Get access Rights & Permissions [Opens in a new window]

Abstract

Recent studies of Echinococcus granulosus molecular strain typing have enabled a better understanding of the transmission cycle of cystic echinococcosis. There have been many publications in this area but there is a need for the evaluation of these tools. We have attempted to respond to this need in our study, which assessed 8 DNA fragments of 40 E. granulosus cysts from North Africa. Parasitological material was collected from 5 types of intermediate hosts, in 5 different countries. The primers chosen to amplify DNA targets were defined either in nuclear DNA, or in mitochondrial DNA. After amplification, PCR products were sequenced. The sequences obtained were aligned and comparisons were made within the group and with GenBank sequences. Whether the target was nuclear or mitochondrial, the same 2 main groups of genotypes were found. The first one, the ‘sheep’ strain, was found in the human, sheep and cattle samples collected in North Africa. The second one, the ‘camel’ strain, was found in the camel cysts and cattle and human cysts from Mauritania. These findings further confirm the congruence of the data given by the nuclear and the mitochondrial genome.

Keywords

Echinococcus granulosusmolecular strain typingnuclear targetmitochondrial target
Type
Research Article
Information
Parasitology , Volume 128 , Issue 2 , February 2004 , pp. 229 - 234
Copyright
2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

BOWLES, J. & McMANUS, D. P. (1993). Molecular variation in Echinococcus. Acta Tropica 53, 291–305.CrossRefGoogle Scholar
BOWLES, J., BLAIR, D. & McMANUS, D. P. (1992). Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–173.CrossRefGoogle Scholar
BOWLES, J., BLAIR, D. & McMANUS, D. P. (1994). Molecular genetic characterization of the cervid strain (‘northern form’) of Echinococcus granulosus. Parasitology 109, 215–221.CrossRefGoogle Scholar
BRETAGNE, S., ASSOULINE, B., VIDAUD, D., HOUIN, R. & VIDAUD, M. (1996). Echinococcus multilocularis: microsatellite polymorphism in U1 snRNA genes. Experimental Parasitology 82, 324–328.CrossRefGoogle Scholar
BRYANT, C. & FLOCKHART, H. A. (1986). Biochemical strain variation in parasitic helminths. Advances in Parasitology 25, 275–319.CrossRefGoogle Scholar
DA SILVA, C. M., FERREIRA, B., PICON, M., GORFINKIEL, N., EHRLICH, R. & ZAHA, A. (1993). Molecular cloning and characterization of actin genes from Echinococcus granulosus. Molecular and Biochemical Parasitology 60, 209–219.CrossRefGoogle Scholar
FROSCH, P. M., MUHLSCHLEGEL, F., SYGULLA, L., HARTMANN, M. & FROSCH, M. (1994). Identification of a cDNA clone from the larval stage of Echinococcus granulosus with homologies to the E. multilocularis antigen EM10-expressing cDNA clone. Parasitology Research 80, 703–705.Google Scholar
GOTTSTEIN, B. & MOWATT, M. R. (1991). Sequencing and characterization of an Echinococcus multilocularis DNA probe and its use in the polymerase chain reaction. Molecular and Biochemical Parasitology 44, 183–193.CrossRefGoogle Scholar
HAAG, K. L., ZAHA, A., ARAUJO, A. M. & GOTTSTEIN, B. (1997). Reduced genetic variability within coding and non-coding regions of the Echinococcus multilocularis genome. Parasitology 115, 521–529.CrossRefGoogle Scholar
HAAG, K. L., ARAUJO, A. M., GOTTSTEIN, B. & ZAHA, A. (1998). Selection, recombination and history in a parasitic flatworm (Echinococcus) inferred from nucleotide sequences. Memorias Institudo Oswaldo Cruz 93, 695–702.CrossRefGoogle Scholar
HAAG, K. L., ARAUJO, A. M., GOTTSTEIN, B., SILES-LUCAS, M., THOMPSON, R. C. & ZAHA, A. (1999). Breeding systems in Echinococcus granulosus (Cestoda; Taeniidae): selfing or outcrossing? Parasitology 118, 63–71.Google Scholar
KAMENETZKY, L., GUTIERREZ, A. M., CANOVA, S. G., HAAG, K. L., GUARNERA, E. A., PARRA, A., GARCIA, G. E. & ROSENZVIT, M. C. (2002). Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infection, Genetics and Evolution 2, 129–136.CrossRefGoogle Scholar
LE, T. H., BLAIR, D. & McMANUS, D. P. (2000). Mitochondrial genomes of human helminths and their use as markers in population genetics and phylogeny. Acta Tropica 77, 243–256.CrossRefGoogle Scholar
LYMBERY, A. J., THOMPSON, R. C. & HOBBS, R. P. (1990). Genetic diversity and genetic differentiation in Echinococcus granulosus (Batsch, 1786) from domestic and sylvatic hosts on the mainland of Australia. Parasitology 101, 283–289.CrossRefGoogle Scholar
MACPHERSON, C. N. & McMANUS, D. P. (1982). A comparative study of Echinococcus granulosus from human and animal hosts in Kenya using isoelectric focusing and isoenzyme analysis. International Journal for Parasitology 12, 515–521.CrossRefGoogle Scholar
MACPHERSON, C. N. L. & WACHIRA, T. W. M. (1997). Cystic echinococcosis in Africa south of the Sahara. In Compendium on Cystic Echinococcosis in Africa and in Middle Eastern Countries with Special Reference to Morocco (ed. Andersen, F. L., Ouhelli, H. & Kachani, M.), pp. 245–277. Brighman Young University Print Services, Provo, UT, USA.
McMANUS, D. P. & BOWLES, J. (1996). Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687–704.CrossRefGoogle Scholar
OKAMOTO, M., BESSHO, Y., KAMIYA, M., KUROSAWA, T. & HORII, T. (1995). Phylogenetic relationships within Taenia taeniaeformis variants and other taeniid cestodes inferred from the nucleotide sequence of the cytochrome c oxidase subunit I gene. Parasitology Research 81, 451–458.CrossRefGoogle Scholar
SNABEL, V., D'AMELIO, S., MATHIOPOULOS, K., TURCEKOVA, L. & DUBINSKY, P. (2000). Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia. Journal of Helminthology 74, 177–181.Google Scholar
THOMPSON, R. C. & LYMBERY, A. J. (1990). Echinococcus: biology and strain variation. International Journal for Parasitology 20, 457–470.CrossRefGoogle Scholar
THOMPSON, R. C. & McMANUS, D. P. (2002). Towards a taxonomic revision of the genus Echinococcus. Trends in Parasitology 18, 452–457.CrossRefGoogle Scholar
TIBAYRENC, M. (1998). Genetic epidemiology of parasitic protozoa and other infectious agents: the need for an integrated approach. International Journal for Parasitology 28, 85–104.CrossRefGoogle Scholar
VAN HERWERDEN, L., GASSER, R. B. & BLAIR, D. (2000). ITS-1 ribosomal DNA sequence variants are maintained in different species and strains of Echinococcus. International Journal for Parasitology 30, 157–169.CrossRefGoogle Scholar
VAWTER, L. & BROWN, W. M. (1986). Nuclear and mitochondrial DNA comparisons reveal extreme rate variation in the molecular clock. Science 234, 194–196.CrossRefGoogle Scholar
ZHANG, L. H., JOSHI, D. D. & McMANUS, D. P. (2000). Three genotypes of Echinococcus granulosus identified in Nepal using mitochondrial DNA markers. Transactions of the Royal Society of Tropical Medicine and Hygiene 94, 258–260.CrossRefGoogle Scholar