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Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1

Published online by Cambridge University Press:  06 May 2004

A. OBWALLER ,
R. SCHNEIDER ,
J. WALOCHNIK ,
B. GOLLACKNER ,
A. DEUTZ ,
K. JANITSCHKE ,
H. ASPÖCK  and
H. AUER
A. OBWALLER
Affiliation:
Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria
R. SCHNEIDER
Affiliation:
Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria
J. WALOCHNIK
Affiliation:
Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria
B. GOLLACKNER
Affiliation:
Department of Surgery, University of Vienna, Vienna, Austria
A. DEUTZ
Affiliation:
Department of Veterinary Affairs, Graz, Austria
K. JANITSCHKE
Affiliation:
Robert Koch Institut, Berlin, Germany
H. ASPÖCK
Affiliation:
Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria
H. AUER
Affiliation:
Department of Medical Parasitology, Clinical Institute of Hygiene and Medical Microbiology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria
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Abstract

Genetic analyses of Echinococcus granulosus isolates from different intermediate host species have demonstrated substantial levels of variation for some genotype (strain) clusters. To determine the range of genetic variability within and between genotypes we amplified and cloned partial cox1 and nadh1 genes from 16 isolates of E. granulosus from 4 continents. Furthermore, we sequenced different clones from a PCR product to analyse the intra-individual genetic variance. The findings showed a moderate degree of variance within single isolates and a significant degree of variance between the cluster of genotypes G1–G3 (sheep, Tasmanian sheep and buffalo strain), genotypes G4 (horse strain) and G5 (cattle strain) and the cluster of the genotypes G6 (camel strain) and G7 (pig strain). The variance of up to 2·2% within genotypes was relatively low compared with that of 4·3–15·7% among genotypes. The present results indicate that a re-examination of the classification of 5 genotypes of Echinococcus is warranted. Hence, our data highly support a re-evaluation of the taxonomy of the clades G1–G3, G4, G5, G6/7 and G8 (cervid strain) within the genus Echinococcus.

Keywords

Echinococcus granulosuscytochrome c oxidase-1 (cox-1)NADH dehydrogenase-1 (nadh1)molecular strain differentiationsequence heterogeneity
Type
Research Article
Information
Parasitology , Volume 128 , Issue 5 , May 2004 , pp. 569 - 575
Copyright
2004 Cambridge University Press

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