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Echinococcus granulosus: in vitro effect of monensin on the tegument of the protoscolex

Published online by Cambridge University Press:  06 April 2009

M. T. Rogan  and
K. Sylvia Richards
M. T. Rogan
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
K. Sylvia Richards
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
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Summary

Protoscoleces of Echinococcus granulosus were incubated in a 10 μm solution of monensin and their viability and the ultrastructure of their tegument were monitored over a 36 h period. The earliest effects of the lonophore, apparent within 15 min, involved a degree of cisternal swelling of the tegumentary Golgi complexes. Swelling became more pronounced with time and cytons eventually contained numerous large electron-lucent vesicles. Residual bodies indicative of autophagy were observed in the cytons and distal cytoplasm from 1 h onwards. All movement of protoscoleces ceased after 1 h and the surface of the soma subsequently became folded, possibly suggesting spastic paralysis. Later, vacuoles and large cytoplasmic blebs were observed in the distal cytoplasm and viability dropped rapidly after 12 h. All protoscoleces were dead at 36 h. The potential of monensin as an in vivo protoscolicidal agent is discussed.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 347 - 355
Copyright
Copyright © Cambridge University Press 1986

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References

Ali-Khan, Z. (1978). Echinococcus multilocularis: cell mediated immune response in early and chronic alveolar murine hydatidosis. Experimental Parasitology 46, 156–65.CrossRefGoogle ScholarPubMed
Annen, J., Köhler, P., Eckert, J. & Speck, S. (1980). Cytotoxic properties of Echinococcus granulosus cyst fluid. In The Host Invader Interplay (ed. H., Van den Bossche), pp. 339–42. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Bortoletti, G. & Ferretti, G. (1973). Investigations on the larval forms of Echinococcus granulosus with the electron microscope. Rivista di Parassitologia 34, 89–110.Google Scholar
Chinnery, J. & Morris, D. L. (1986). Effect of albendazole sulphoxide on viability of hydatid protoscoleces in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene. (in the Press).CrossRefGoogle Scholar
Clarkson, J. & Erasmus, D. A. (1984). Schistosoma mansoni: an in vivo study of drug-indicated autophagy in the gastrodermis. Journal of Helminthology 58, 59–68.CrossRefGoogle Scholar
Dew, H. R. (1922). Observations on the mode of development of brood capsules and scolices in the encysted stage of Taenia echinococcus. Medical Journal of Australia 2, 381–4.CrossRefGoogle Scholar
Ellinger, A. & Povelka, M. (1984). Effect of monensin on the Golgi apparatus of absorptive cells in the small intestine of the rat. Morphological and cytochemical studies. Cell and Tissue Research 235, 187–94.CrossRefGoogle ScholarPubMed
Fairweather, I. & Skuce, P. J. (1985). An evaluation of the fasciolicidal potential of monensin. J. The effect of monensin on the neuromuscular and osmoregulatory systems of Fasciola hepatica. Proceedings of the British Society for Parasitology, Abstract No. 138. Parasitology 91, 420.Google Scholar
Griffiths, G., Quinn, P. & Warren, G. (1983). Dissection of the Golgi complex. I. Monensin inhibits the transport of viral membrane proteins from medial to trans Golgi cisternae in baby hamster kidney cells infected with Semliki Forest virus. Journal of Cell Biology 96, 835–50.CrossRefGoogle ScholarPubMed
Kassis, A. I., Goh, S. L. & Tanner, C. E. (1976). Lesions induced by complement in vitro on the protoscoleces of Echinococcus multilocularis: a study by electron microscopy. International Journal for Parasitology 6, 199–211.CrossRefGoogle ScholarPubMed
Lopez-Campos, J. L., Linares, J., Alonso, J., Aneiros, J. & Diaz-Flores, L. (1978). Estudio de la capa laminada, membranas germinativas y tegumento del protoscolex del Echinococcus granulosus en su localizacion humana. Morfologia normal y patologia 2, 99–109.Google Scholar
Marchiondo, A. A. & Andersen, F. L. (1983). Fine structure and freeze-etch study of the protoscolex tegument of Echinococcus multilocularis (Cestoda). Journal of Parasitology 69, 709–18.CrossRefGoogle ScholarPubMed
Marchiondo, A. A. & Andersen, F. L. (1985). In vivo efficacy and ultrastructural effects of mitomycin C against experimental alveolar hydatid disease. Journal of Helminthology 59, 29–38.CrossRefGoogle ScholarPubMed
Marshall, I. & Edwards, G. T. (1982). The effects of sustained release praziquantel on the survival of protoscolices of Echinococcus granulosus equinus in laboratory mice. Annals of Tropical Medicine and Parasitology 76, 649–51.CrossRefGoogle ScholarPubMed
Morris, D. L. (1983). Chemotherapy of hydatid disease. Journal of Antimicrobial Chemotherapy 11, 494–6.CrossRefGoogle ScholarPubMed
Morseth, D. J. (1967). Fine structure of the hydatid cyst and protoscolex of Echinococcus granulosus. Journal of Parasitology 53, 312–25.CrossRefGoogle ScholarPubMed
Pennoit-De Cooman, E. & De Rycke, P. H. (1972). Experimental secondary echinococcosis of Echinococcus granulosus. III. Observations on the morphogenesis of protoscoleces. Biologishe Jaarboek Dodonaea 40, 276–87.Google Scholar
Richards, K. S. (1984). Echinococcus granulosus equinus: the histochemistry of the laminated layer of the hydatid cyst. Folia Histochemica et Cytochemica 22, 21–32.Google ScholarPubMed
Richards, K. S., Arme, C. & Bridges, J. F. (1983). Echinococcus granulosus equinus: an ultra-structural study of the laminated layer, including changes on incubating cysts in various media. Parasitology 86, 399–405.CrossRefGoogle Scholar
Richards, K. S., Arme, C. & Bridges, J. F. (1984). Echinococcus granulosus equinus: variation in the germinal layer of murine hydatids and evidence of autophagy. Parasitology 89, 35–47.CrossRefGoogle ScholarPubMed
Robinson, R. D. & Arme, C. (1985). Echinococcus granulosus: studies on the interaction of protoscoleces with murine peritoneal macrophages. Proceedings of the British Society for Parasitology, Abstract No. 98. Parasitology 91, 417.Google Scholar
Schantz, P. M., Van den Bossche, H. & Eckert, J. (1982). Chemotherapy for larval echinococcosis in animals and humans: report of a workshop. Zeitschrift für Parasitenkunde 67, 5–26.CrossRefGoogle ScholarPubMed
Shumard, R. F., Callender, M. E. & Reid, W. M. (1970). Monensin a new anticoccidial agent. Fourteenth World Poultry Congress, Madrid, 6–1209 1970. Pathology 3, 421–7.Google Scholar
Skuce, P. J. & Fairweather, I. (1985). The effect of monensin on vitelline cell ultrastructure and egg production in Fasciola hepatica, and the results of efficacy studies in the laboratory rat. Proceedings of the British Society for Parasitology, Abstract no. 139. Parasitology 91, 420.Google Scholar
Smith, C. K. II., Galloway, R. B. & White, S. L. (1981). Effect of ionophores on survival, penetration, and development of Eimeria tenella sporozoites in vitro. Journal of Parasitology 67, 511–16.CrossRefGoogle ScholarPubMed
Strous, G. S., Van Kerkhof, P., Willemsen, R., Slot, J. W. & Geuze, H. J. (1985). Effect of monensin on the metabolism, localization and biosynthesis of N- and O- linked oligosaccharides of galactosyltransferase. European Journal of Cell Biology 36, 256–62.Google Scholar
Tartakoff, A. M. (1983). Perturbation of vesicular traffic with the carboxylic ionophore monensin. Cell 32, 1026–8.CrossRefGoogle ScholarPubMed
Thomas, H. & Gönnert, R. (1978). The efficacy of praziquantel against cestodes in cats, dogs and sheep. Research in Veterinary Science 24, 20–5.CrossRefGoogle ScholarPubMed
Thompson, R. C. A. (1976). The development of brood capsules and protoscoleces in secondary hydatid cysts of Echinococcus granulosus. Zeitschrift für Parasitenkunde 51, 31–6.CrossRefGoogle Scholar
Threadgold, L. T. (1976). The Ultrastructure of the Animal Cell. Oxford: Pergamon Press.Google Scholar
Threadgold, L. T. & Dunn, J. (1983). Taenia crassiceps: regional variations in ultrastructure and evidence of endocytosis in the cysticercus tegument. Experimental Parasitology 55, 121–31.CrossRefGoogle ScholarPubMed
Threadgold, L. T. & Hopkins, C. A. (1981). Schistocephalus solidus and Ligula intestinalis: pinocytosis by the tegument. Experimental Parasitology 51, 444–56.CrossRefGoogle ScholarPubMed
Todd, G. C., Novilla, M. N. & Howard, L. C. (1984). Comparative toxicology of monensin sodium in laboratory animals. Journal of Animal Science 58, 1512–17.CrossRefGoogle ScholarPubMed
Verheyen, A. (1982). Echinococcus granulosus: the influence of mebendazole therapy on the ultrastructural morphology of the germinal layer of hydatid cysts in humans and mice. Zeitschrift für Parasitenkunde 67, 55–65.CrossRefGoogle Scholar
Yao Ping, L., Jun, L. & Xun, D. S. (1985). Praziquantel in treatment and investigation with abdominal hydatidosis. Preliminary report of animal experiments and evaluation of 101 clinical cases. XIII Congreso International de Hidatidologia, Madrid, abstact 234.Google Scholar