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Echinococcus granulosus equinus: variation in the germinal layer of murine hydatids and evidence of autophagy

Published online by Cambridge University Press:  06 April 2009

K. Sylvia Richards ,
C. Arme  and
Janine F. Bridges
K. Sylvia Richards
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
C. Arme
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
Janine F. Bridges
Affiliation:
Parasitology Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffs. ST5 5BG
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Summary

The germinal layer of sterile 9-month-old murine peritoneal cysts of Echinococcus granulosus equinus shows interrelated variation in depth, tissue integrity, metabolic reserves and the number of autophagic lamellar bodies present. These features are similar in large and medium-sized cysts from the same host, whether occurring singly or within cyst masses. Deep germinal layers (> 16 μm) are lipid- and glycogen-rich and possess numerous autophagic vacuoles with 6 nm period lamellar stacks asymmetrically disposed peripherally; shallow layers (< 12 μm), with indications of degeneration, have depleted metabolic reserves and fewer lamellar bodies. These bodies are formed by smooth endoplasmic reticulum encirclement of small glycogen masses followed by further sequestration, and eventually definition of glycogen particles may be lost. Autophagy of mitochondria and cytoplasmic vesicles also occurs. The presence of lysosomal enzymes within the layer suggests autolysosomal compartmentalization of excess substrate and effete material. Mucopolysaccharide bodies, containing material similar to that exocytosed to form the laminated layer matrix, occur and are formed from fusion and autophagy of Golgi-derived vesicles. These bodies may also develop peripheral 6 nm period lamellar stacks, but of limited depth. Mucopolysaccharide bodies are the dominant feature of the germinal layer of very small cyst-mass cysts in which laminated layer production is considered to be arrested. They thus represent a repository for the unreleased mucopolysaccharide material.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 1 , August 1984 , pp. 35 - 48
Copyright
Copyright © Cambridge University Press 1984

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References

Bayne, B. L., Bubel, A., Gabbott, P. A., Livingstone, D. R., Lowe, D. M. & Moore, M. N. (1982). Glycogen utilisation and gametogenesis in Mytilus edulis L. Marine Biology Letters 3, 89–105.Google Scholar
Bortoletti, G. & Diaz, G. (1978). Sterological investigation on the increase in surface area due to the microtriches of the hydatid cyst in different organs and in different hosts. International Journal for Parasitology 8, 433–6.CrossRefGoogle Scholar
Bortoletti, G. & Ferretti, G. (1973). Investigations on the larval forms of Echinococcus granulosus with the electron microscope. Rivista di Parassitologia 34, 89–110.Google Scholar
Bortoletti, G. & Ferretti, G. (1978). Ultrastructural aspects of fertile and sterile cysts of Echinococcus granulosus developed in hosts of different species. International Journal for Parasitology 8, 421–31.CrossRefGoogle ScholarPubMed
Bowen, I. D. (1973). Glycosidases, β-glucosidases, β-glucuronidase. In Electron Microscopy of Enzymes, vol. 1 (ed. Hayat, M. A.), pp. 77–103. New York: Van Nostrand Reinhold.Google Scholar
Doty, S. B., Smith, C. E., Hand, A. R. & Oliver, C. (1977). Inorganic trimetaphosphatase as a histochemical marker for lysosomes in light and electron microscopy. Journal of Histochemistry and Cytochemistry 25, 1381–4.CrossRefGoogle ScholarPubMed
Essner, E. (1973). Phosphatases. In Electron Microscopy of Enzymes, vol. 1 (ed. Hayat, M. A.), pp. 44–76. New York: Van Nostrand Reinhold.Google Scholar
Geddes, R. & Stratton, G. C. (1977). The influence of lysosomes on glycogen metabolism. The Biochemical Journal 163, 193–200.CrossRefGoogle ScholarPubMed
Hers, H. G. & de Barsy, T. (1973). Type II glycogenosis (acid maltase deficiency). In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 197–216. New York and London: Academic Press.Google Scholar
Hopsu-Havu, V. K. & Helminen, H. (1973). Sulfatases. In Electron Microscopy of Enzymes, vol. 2 (ed. Hayat, M. A.), pp. 90–109. New York: Van Nostrand Reinhold.Google Scholar
Kilejian, A., Schinazi, L. A. & Schwabe, C. W. (1961). Host–parasite relationships in echinococcosis. V. Histochemical observations on Echinococcus granulosus. Journal of Parasitology 47, 181–8.CrossRefGoogle ScholarPubMed
Kilejian, A. & Schwabe, C. W. (1971). Studies on the polysaccharides of the Echinococcus granulosus cyst, with observations on a possible mechanism for laminated membrane formation. Comparative Biochemistry and Physiology 40 B, 25–36.Google Scholar
Kint, J. A. & Carton, D. (1973). Fabry's disease. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 357–80. New York and London: Academic Press.Google Scholar
Lascano, E. F., Coltorti, E. A. & Varela-Diaz, V. M. (1975). Fine structure of the germinal membrane of Echinococcus granulosus cysts. Journal of Parasitology 61, 853–60.CrossRefGoogle ScholarPubMed
Leaback, D. H. & Walker, P. G. (1961). Studies on glucosaminidase. 4. The fluorimetric assay of N-acetyl-β-glucosaminidase. The Biochemical Journal 78, 151–6.CrossRefGoogle ScholarPubMed
Lewis, R. P. & Knight, D. P. (1977). Staining methods for sectioned material. Practical Methods in Electron Microscopy, vol. 5 (ed. Glauert, A. M.), Part 1. Amsterdam: North-Holland.Google Scholar
Morseth, D. J. (1967). Fine structure of the hydatid cyst and protoscolex of Echinococcus granulosus. Journal of Parasitology 53, 312–25.CrossRefGoogle ScholarPubMed
Novikoff, A. B. (1973). Lysosomes: a personal account. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 1–41. New York and London: Academic Press.Google Scholar
Richards, K. S. (1984). Echinococcus granulosus equinus: the histochemistry of the laminated layer of the hydatid cyst. Folia Histochemica et Cytochemica (in the Press).Google Scholar
Richards, K. S., Arme, C. & Bridges, J. F. (1983 a). Echinococcus granulosus equinus: an ultrastructural study of murine tissue response to hydatid cysts. Parasitology 86, 407–17.CrossRefGoogle ScholarPubMed
Richards, K. S., Arme, C. & Bridges, J. F. (1983 b). Echinococcus granulosus equinus: an ultrastructural study of the laminated layer, including changes on incubating cysts in various media. Parasitology 86, 399–405.CrossRefGoogle ScholarPubMed
Rowley, A. F. (1979). An ultrastructural study of membranous inclusions found in the haemocytes of the stick insect Clitumnus extradentatus. Acta Zoologica (Stockholm) 60, 217–22.Google Scholar
Schwabe, C. W., Kilejian, A. & Lainas, G. (1970). The propagation of secondary cysts of Echinococcus granulosus in the Mongolian jird, Meriones unguiculatus. Journal of Parasitology 56, 80–3.CrossRefGoogle ScholarPubMed
Singh, G. & Lee, R. E. (1979). Ultrastructure of hydatid cyst. Archives of Pathology and Laboratory Medicine 103, 459–62.Google ScholarPubMed
Thomas, J. A. & Kothare, S. N. (1975). Tissue response in hydatidosis. Indian Journal of Medical Research 63, 1761–6.Google ScholarPubMed
Threadgold, L. T. & Arme, C. (1974). Electron microscope studies of Fasciola hepatica. XI. Autophagy and parenchymal cell function. Experimental Parasitology 35, 389–405.CrossRefGoogle ScholarPubMed
Vaes, G. (1973). Digestive capacity of lysosomes. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 43–77. New York and London: Academic Press.Google Scholar
Van Hoof, F. (1973). Mucopolysaccharidoses. In Lysosomes and Storage Diseases (ed. Hers, H. G. and Van Hoof, F.), pp. 217–59. New York and London: Academic Press.Google Scholar
Vatter, A. E., Zambernard, J. & Priest, J. (1966). Cell structure. In Ultrastructural Aspects of Disease (ed. King, D. W.), pp. 16–35. New York, Evanston and London: Hoeber Medical Division, Harper and Row.Google Scholar
Verheyen, A. (1982). Echinococcus granulosus: the influence of mebendazole therapy on the ultrastructural morphology of the germinal layer of hydatid cysts in humans and mice. Zeitschrift für Parasitenkunde 67, 55–65.CrossRefGoogle Scholar