Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-30T23:57:13.386Z Has data issue: false hasContentIssue false
  • English
  • Français

Echinococcus granulosus: development in vitro from oncosphere to immature hydatid cyst

Published online by Cambridge University Press:  06 April 2009

D. D. Heath  and
S. B. Lawrence
D. D. Heath
Affiliation:
Wallaceville Animal Research Centre, Research Division, Ministry of Agriculture and Fisheries, Private Bag, Upper Hutt, New Zealand
S. B. Lawrence
Affiliation:
Wallaceville Animal Research Centre, Research Division, Ministry of Agriculture and Fisheries, Private Bag, Upper Hutt, New Zealand
Get access Rights & Permissions [Opens in a new window]

Extract

Echinococcus granulosus cysts were cultured in vitro from the oncosphere, and cyst morphogenesis is described. Cysts grew to a mean diameter of 16 mm in 120 days, with a maximum of 20 mm. An amorphous layer was apparent around the larvae during early post-oncospheral re-organization. Organisms became vesicular at 6 days and laminations appeared in the amorphous layer at 10 days. Confluence of proliferating cells to form the syncytium of the germinal membrane was observed.

Type
Research Article
Information
Parasitology , Volume 73 , Issue 3 , December 1976 , pp. 417 - 423
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bénex, J. (1968). Considérations expérimentales nouvelles sur l'évolution in vitro en milieu liquide des larves d'Echinococcus granulosus. Annales de Parasitologie (Paris) 43, 561–72.Google ScholarPubMed
Brudnjak, J., Cvetnic, S. & Wikerhauser, T. (1970). Cystic development of the protoscoleces and brood capsules of Echinococcus granulosus in cell cultures and cell-free media. Veterinarski Arhiv (Zagreb) 40, 292–6.Google Scholar
Heath, D. D. (1973 a). An improved technique for the in vitro culture of taeniid larvae. International Journal for Parasitology 3, 481–4.CrossRefGoogle ScholarPubMed
Heath, D. D. (1973 b). The life cycle of Echinococcus granulosus - a review. In Recent Advances in Hydatid Disease. Symposium Proceedings. Hamilton Medical Veterinary Association 7–18.Google Scholar
Heath, D. D. & Osborn, P. J. (1976). Formation of Echinococcus granulosus laminated membrane in a denned medium. International Journal for Parasitology (in the Press).CrossRefGoogle Scholar
Heath, D. D. & Pavloff, P. (1975). The fate of Taenia taeniaeformis oncospheres in normal and passively protected rats. International Journal for Parasitology 5, 82–8.CrossRefGoogle ScholarPubMed
Heath, D. D. & Smyth, J. D. (1970). In vitro cultivation of Echinococcus granulosus, Taenia hydatigena, T. ovis, T. pisiformis and T. serialis from oncosphere to cystic larva. Parasitology 61, 329–43.CrossRefGoogle Scholar
Lumsden, R. D. (1975). Surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology 37, 267–339.CrossRefGoogle ScholarPubMed
Smyth, J. D. (1962). Studies on tapeworm physiology. X. Axenic cultivation of the hydatid organism, Echinococcus granulosus: establishment of a basic technique. Parasitology 52, 331–457.CrossRefGoogle Scholar
Varela-Diaz, V. M. & Coltorti, E. A. (1973). The presence of host immunoglobulins in hydatid cyst membranes. Journal of Parasitology 59, 484–8.CrossRefGoogle ScholarPubMed