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DNA synthesis in gametocytes of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

C. J. Janse ,
T. Ponnudurai ,
A. H. W. Lensen ,
J. H. E. Th. Meuwissen ,
J. Ramesar ,
M. Van Der Ploeg  and
J. P. Overdulve
C. J. Janse
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht
T. Ponnudurai
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
A. H. W. Lensen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
J. Ramesar
Affiliation:
Laboratory of Parasitology (Medical Faculty), University of Leiden, Wassenaarseweg 62, P.O. Box 9605, 2300 RC Leiden, The Netherlands
M. Van Der Ploeg
Affiliation:
Department of Histochemistry and Cytochemistry, University of Leiden, Wassenaarseweg 72, P.O. Box 9503, 2300 RA Leiden, The Netherlands
J. P. Overdulve
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht
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Summary

The DNA content of Plasmodium falciparum gametocytes during intra-erythrocytic development and during gametogenesis was established by cytophotometric methods. Intra-erythrocytic micro- and macrogametocytes (Stage I-Stage VB) contain about twice the amount of DNA of haploid sporozoites and ringstages, indicating that DNA is synthesized during transformation of ringforms into Stage I gametocytes. Microgametocytes, after activation at pH 8, rapidly duplicate their genome several times, while the DNA content of macrogametocytes remains constant during gametogenesis.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1988

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References

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