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DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp.

Published online by Cambridge University Press:  06 April 2009

W. T. Artama ,
M. W. Agey  and
J. E. Donelson
W. T. Artama
Affiliation:
IUC Biotechnology, University of Gadjah Mada, Jl. Teknika Utara, BarekYogyakarta, Indonesia Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
M. W. Agey
Affiliation:
Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
J. E. Donelson
Affiliation:
Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
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Summary

Two clones from separate isolates of Trypanosoma evansi in Indonesia were found by polymerase chain reaction (PCR) analyses to contain 3 different repeated nuclear DNA sequences of Trypanosoma brucei spp: the consensus sequence for a highly repetitive 177 base pairs and the gene repeats encoding procyclin and the spliced leader. In addition, the 994 bp minicircle sequence of one of the clones was determined, and PCR amplification primers specific for minicircles of T. evansi were identified that do not amplify minicircle sequences in the T. brucei spp. clones tested.

Keywords

Trypanosoma evansiminicircle DNApolymerase chain reactiongene repeats
Type
Research Article
Information
Parasitology , Volume 104 , Issue 1 , February 1992 , pp. 67 - 74
Copyright
Copyright © Cambridge University Press 1992

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