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DNA base composition of filarial nematodes

Published online by Cambridge University Press:  06 April 2009

N. Rothstein ,
T. J. Stoller  and
T. V. Rajan
N. Rothstein
Affiliation:
Department of PathologyAlbert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461
T. J. Stoller
Affiliation:
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461
T. V. Rajan
Affiliation:
Department of PathologyAlbert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461
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Summary

We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal denaturation and direct analysis of base content by HPLC following enzymatic hydrolysis, reveal that the GC content of filarial nematodes is 26–28%. We have been unable to find methylcytosine in the DNA of B. malayi.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 1 , August 1988 , pp. 75 - 79
Copyright
Copyright © Cambridge University Press 1988

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References

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