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Dissection of the hierarchy and synergism of the bile derived signal on Cryptosporidium parvum excystation and infectivity

Published online by Cambridge University Press:  16 August 2012

B. J. KING ,
A. R. KEEGAN ,
R. PHILLIPS ,
S. FANOK  and
P. T. MONIS
B. J. KING*
Affiliation:
Australian Water Quality Centre, SA Water Corporation, Adelaide, South Australia, Australia, 5000
A. R. KEEGAN
Affiliation:
Australian Water Quality Centre, SA Water Corporation, Adelaide, South Australia, Australia, 5000
R. PHILLIPS
Affiliation:
Australian Water Quality Centre, SA Water Corporation, Adelaide, South Australia, Australia, 5000
S. FANOK
Affiliation:
Australian Water Quality Centre, SA Water Corporation, Adelaide, South Australia, Australia, 5000
P. T. MONIS
Affiliation:
Australian Water Quality Centre, SA Water Corporation, Adelaide, South Australia, Australia, 5000
*
*Corresponding author: Tel:61 8 742 42114. Fax: 61 8 700 32114. E-mail: [email protected]
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Summary

Bile salts have been identified as an important trigger for excystation of Cryptosporidium oocysts but the hierarchy or synergism of this signal in relation to other triggers involved in excystation is poorly understood. In addition to excystation, bile salts have also been reported to increase the invasiveness of sporozoites within in vitro culture, possibly by affecting the secretory pathway via modification of intracellular calcium signalling. Nevertheless, incorporation of bile or bile salts into in vitro assays is not universal, with recent reports of negative effects on parasite growth. Here we report that bile and sodium taurocholate significantly affect both excystation rate and parasite in vitro growth. We demonstrate that their effect on excystation is dose, time and pre-treatment temperature dependent, while increases in parasite replication appear to be associated with modulation of parasite intracellular calcium and increased host cell susceptibility to infection. Notably, we illustrate that bile has a significant effect on host cells and can be cytotoxic at concentrations not much higher than those currently used for in vitro assays. This work should assist with more rational design of in vitro culture systems, with significant considerations for assay format when incorporating bile or bile salts as an excystation trigger.

Keywords

Cryptosporidiumbile acidsexcystationin vitro infectivityintracellular calcium
Type
Research Article
Information
Parasitology , Volume 139 , Issue 12 , October 2012 , pp. 1533 - 1546
Copyright
Copyright © Cambridge University Press 2012

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