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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential

Published online by Cambridge University Press:  19 October 2006

U. PARKAR ,
R. J. TRAUB ,
S. KUMAR ,
M. MUNGTHIN ,
S. VITALI ,
S. LEELAYOOVA ,
K. MORRIS  and
R. C. A. THOMPSON
U. PARKAR
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the State Agricultural Biotechnology Centre, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Western Australia 6150, Australia
R. J. TRAUB
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the State Agricultural Biotechnology Centre, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Western Australia 6150, Australia
S. KUMAR
Affiliation:
Faculty of Medicine, University of Malaya, Kuala Lumpur, Selangor 50603, Malaysia
M. MUNGTHIN
Affiliation:
Department of Parasitology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand
S. VITALI
Affiliation:
Perth Zoo, South Perth, Western Australia 6151, Australia
S. LEELAYOOVA
Affiliation:
Department of Parasitology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand
K. MORRIS
Affiliation:
Science Division, Department of Conservation and Land Management, Wildlife Place, Woodvale WA 6026, Australia
R. C. A. THOMPSON
Affiliation:
WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and the State Agricultural Biotechnology Centre, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Western Australia 6150, Australia
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Abstract

In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.

Keywords

Blastocystisin vitro amplificationzoonosesmolecular characterization
Type
Research Article
Information
Parasitology , Volume 134 , Issue 3 , March 2007 , pp. 359 - 367
Copyright
© 2006 Cambridge University Press

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