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Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Published online by Cambridge University Press:  06 April 2009

M. A. Ouaissi ,
A. Haque  and
A. Capron
M. A. Ouaissi
Affiliation:
Centre d'Immunologie et de Biologìe Parasitaire (INSERM U 167), Institut Pasteur, B.P. 3415, 59019 Lille Cédex, France
A. Haque
Affiliation:
Centre d'Immunologie et de Biologìe Parasitaire (INSERM U 167), Institut Pasteur, B.P. 3415, 59019 Lille Cédex, France
A. Capron
Affiliation:
Centre d'Immunologie et de Biologìe Parasitaire (INSERM U 167), Institut Pasteur, B.P. 3415, 59019 Lille Cédex, France
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Summary

The in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 1 , February 1981 , pp. 55 - 62
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Amstrong, J. A. & D'arcy Hart, P. (1971) Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. Journal of Experimental Medicine 134, 713–40.Google Scholar
Belcher, R. W., Carney, J. F. & Monahan, F. G. (1973) An electron microscopic study of phagocytosis of Candida albicans by polymorphonuclear leukocytes. Laboratory Investigation 29, 620–7.Google ScholarPubMed
Capron, A., Dessaint, J. P., Joseph, M., Rousseaux, R. & Bazin, H. (1977) Interaction between IgE complexes and macrophages in the rat: a new mechanism of macrophage activation. European Journal of Immunology 7, 315–22.Google Scholar
Capron, A., Dessaint, J. P., Capron, M. & Bazin, H. (1975) Specific IgE antibodies in immune adherence of normal macrophages to Schistosoma mansoni schistosomules. Nature, London 253, 474–5.CrossRefGoogle ScholarPubMed
Chandi, S. M. & Job, C. K. (1978) The early cellular response to Mycobacterium leprae. An ultrastructural study. Leprosy in India 50, 345–51.Google ScholarPubMed
Cohn, Z. A. (1968) The structure and function of monocytes and macrophages. Advances in Immunology 9, 163–214.CrossRefGoogle ScholarPubMed
de Harven, E. (1967) Methods in electron microscopic cytology. In Methods in Cancer Research (ed. Busch, H.), vol. 1, pp. 3–44. London and New York: Academic Press.Google Scholar
Dumont, A. & Robert, A. (1970) Electron microscope study of phagocytosis of Histoplasma capsulatum by hamster peritoneal macrophages. Laboratory Investigation 23, 278–86.Google ScholarPubMed
Dwight, A., Powell, M. D. & Kenneth, A. M. (1977) Scanning electron microscopy of guinea pig alveolar macrophages. In vitro phagocytosis of Mycoplasma pneumoniae. Laboratory Investigation 37 (6), 535–43.Google Scholar
Green, G.M. & Kass, E. H. (1964) The role of the alveolar macrophage in the clearance of bacteria from the lung. Journal of Experimental Medicine 119, 167–76.Google Scholar
Haque, A., Joseph, M., Ouaissi, M. A., Capron, M. & Capron, A. (1980) Macrophage cytotoxicity by IgE against microfilaria. Clinical Experimental Immunology 40, 487–95.Google ScholarPubMed
Haque, A., Lefebvre, M. N., Ogilvie, B. M. & Capron, A. (1978) Dipetalonema viteae in hamsters: effect of antiserum or immunization with parasite extract on production of microfilariae. Parasitology 76, 77–88.CrossRefGoogle ScholarPubMed
Horn, R. G., Koenig, M. G., Goodman, J. S. & Collins, R. D. (1969) Phagocytosis of Staphylococcus aureus by hepatic reticuloendothelial cells: an ultra-structural study. Laboratory Investigation 21, 406–14.Google Scholar
Jones, T. C. & Hirsch, J. G. (1971) The interaction in vitro of Mycoplasma pulmonis with mouse peritoneal macrophages and T-cells. Journal of Experimental Medicine 133, 231–59.Google Scholar
Jones, T. C., Yeh, S. & Hirsch, J. G. (1972 a). Studies on attachment and ingestion phases of phagocytosis of Mycoplasma pulmonis by mouse peritoneal macrophages. Proceedings of the Society for Experimental Biology and Medicine 139, 464–70.Google Scholar
Jones, T. C., Yeh, S. & Hirsch, J. G. (1972 b). The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite. Journal of Experimental Medicine 136, 1157–72.CrossRefGoogle ScholarPubMed
McLaren, D. J., Ramalho-Pinto, F. J. & Smithers, S. R. (1978) Ultrastructural evidence for complement and antibody-dependent damage to schistosomula of Schistosma mansoni by rat eosinophils in vitro. Parasitology 77, 313–24.Google Scholar
Millonig, G. (1961) Advantages of a phosphate buffer for osmium tetroxide solutions in fixation. Journal of Applied Physiology 32, 1637.Google Scholar
Novikoff, A. B. & Novikoff, P. M. (1977) Cytochemical contributions to differentiating GERL from the Golgi Apparatus. Histochemical Journal 9, 525–51.CrossRefGoogle ScholarPubMed
Pugh-Humphreys, R. G. P. & Thomson, A. W. (1979) An ultrastructural study of peritoneal mononuclear phagocytes from Corynebacterium parvum-injected mice. British Journal of Experimental Pathology 60, 259–68.Google Scholar
Subrahmanyam, D., Rao, Y. V. B. G., Mehta, K. & Nelson, D. S. (1976) Serum-dependent adheshion and cytotoxicity of cells to Litomosoides carinii microfilariae. Nature, London 260, 529–30.Google Scholar
Tanner, M. & Weiss, N. (1978) Studies on Dipetalonema viteae (Filarioidea). II. Antibody- dependent adhesion of peritoneal exudate cells to microfilaria in vitro. Acta Tropica 35, 151–60.Google Scholar
Thump, B. F. & Arstila, A. U. (1971) Cell injury and cell death. In Principles of Pathology (ed. Lavia, M. F. and Hill, R. B.), p. 9. New York, London and Toronto: Oxford University Press.Google Scholar
Worms, M. J., Terry, R. J. & Terry, A. (1961) Dipetalonema viteae filarial parasite of the jird, Merione libycus. I. Maintenance in the laboratory. Journal of Parasitology 47, 963–70.CrossRefGoogle Scholar