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Differential expression of AP-1 transcription factor genes c-fos and c-jun in the helminth parasites Taenia crassiceps and Taenia solium

Published online by Cambridge University Press:  06 August 2004

J. MORALES-MONTOR ,
G. ESCOBEDO ,
M. RODRIGUEZ-DORANTES ,
N. TÉLLEZ-ASCENCIO ,
M. A. CERBÓN  and
C. LARRALDE
J. MORALES-MONTOR
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
G. ESCOBEDO
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
M. RODRIGUEZ-DORANTES
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
N. TÉLLEZ-ASCENCIO
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
M. A. CERBÓN
Affiliation:
Departamento de Biología, Facultad de Química, U.N.A.M. AP 70228, México D.F. 04510, México
C. LARRALDE
Affiliation:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, U.N.A.M. AP 70228, México D.F. 04510, México
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Abstract

Homologues of c-fos and c-jun from total DNA of Taenia crassiceps and Taenia solium were cloned and sequenced. The amino acid alignment analysis revealed that c-fos DNAs from T. crassiceps and T. solium were highly homologous (96%), and both have high homology compared to several mammalian c-fos proteins (93% to mouse, 96% to rat and 86% to human). The c-jun protein alignment showed higher homology (T. crassiceps and T. solium have 98%), when compared with mouse, rat and human, being 92%, 98% and 93% respectively. RT–PCR amplification of the parasite's total RNA, showed that T. crassiceps expressed both AP-1 complex genes, while T. solium only expressed c-fos. Southern blot hybridization analysis confirmed the true origin of each amplified gene. AP-1 transcription gene expression is regulated by oestradiol in the same fashion as their mammalian counterparts only in T. crassiceps. To study if AP-1 genes are involved in a physiological function of the cyst, reproduction was studied in vitro. Oestradiol treatment stimulated reproduction in T. crassiceps but not in T. solium cysticerci. This is the first report of the detection and functionality of AP-1 transcription factor genes in any species of helminth parasite.

Keywords

AP-1 transcription factorTaenia crassicepsTaenia soliumc-fos genec-jun gene
Type
Research Article
Information
Parasitology , Volume 129 , Issue 2 , August 2004 , pp. 233 - 243
Copyright
2004 Cambridge University Press

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