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Developmental arrest in Caenorhabditis elegans dauer larvae causes high expression of enzymes involved in thymidylate biosynthesis, similar to that found in Trichinella muscle larvae

Published online by Cambridge University Press:  23 May 2005

P. WIŃSKA ,
B. GOŁOS ,
J. CIEŚLA ,
Z. ZIELIŃSKI ,
T. FRĄCZYK ,
E. WAŁAJTYS-RODE  and
W. RODE
P. WIŃSKA
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
B. GOŁOS
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
J. CIEŚLA
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
Z. ZIELIŃSKI
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
T. FRĄCZYK
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
E. WAŁAJTYS-RODE
Affiliation:
Rzeszów University of Technology Faculty of Chemistry, 3 Powstańców Warszawy Street, 35-959 Rzeszów, Poland
W. RODE
Affiliation:
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
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Abstract

Crude extract specific activities of thymidylate synthase, dUTPase, thymidine kinase and dihydrofolate reductase were high during the development of Caenorhabditis elegans, the dauer larva activities being similar to those previously determined in Trichinella spiralis and T. pseudospiralis muscle larvae (with the exception of thymidine kinase, not detected in Trichinella). High thymidylate synthase expression in developmentally arrested larvae, demonstrated also at the mRNA and protein levels, is in agreement with a global cell cycle arrest of dauer larvae and indicates this unusual cell cycle regulation pattern can be shared by developmentally arrested larvae of C. elegans and the two Trichnella species. Hence, the phenomenon may be characteristic for developmentally arrested larvae of different nematodes, rather than specific for the parasitic Trichinella muscle larvae. Endogenous C. elegans thymidylate synthase was purified and its molecular properties compared with those of the recombinant protein, expression of the latter in E. coli cells confirming the NCBI database sequence identity.

Keywords

thymidylate synthasedUTPasethymidine kinasedihydrofolate reductaseCaenorhabditis elegansTrichinella sppdevelopmental arrestdauer larvamuscle larvacell cycle
Type
Research Article
Information
Parasitology , Volume 131 , Issue 2 , August 2005 , pp. 247 - 254
Copyright
2005 Cambridge University Press

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