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Development of the microsporidian parasite, Loma salmonae, in a rainbow trout gill epithelial cell line (RTG-1): evidence of xenoma development in vitro

Published online by Cambridge University Press:  01 December 2014

S. H. McCONNACHIE ,
J. SHEPPARD ,
G. M. WRIGHT  and
D. J. SPEARE
S. H. McCONNACHIE*
Affiliation:
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada
J. SHEPPARD
Affiliation:
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada
G. M. WRIGHT
Affiliation:
Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3 Canada
D. J. SPEARE
Affiliation:
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada
*
* Corresponding author. Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. E-mail: [email protected]
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Summary

Growth and propagation of fish-infecting microsporidians within cell culture has been more difficult to achieve than for insect- and human-infecting microsporidians. Fish microsporidia tend to elicit xenoma development rather than diffuse growth in vivo, and this process likely increases host specificity. We present evidence that the fish microsporidian, Loma salmonae, has the capacity to develop xenomas within a rainbow trout gill epithelial cell line (RTG-1). Spore numbers increased over a 4 weeks period within cell culture flasks. Xenoma-like structures were observed using phase contrast microscopy, and then confirmed using transmission electron microscopy. Optimization of the L. salmonae-RTG-1 cell model has important implications in elucidating the process of xenoma development induced by microsporidian parasites.

Keywords

microsporidiaLoma salmonaexenomacell culturefish parasite
Type
Research Article
Information
Parasitology , Volume 142 , Issue 2 , February 2015 , pp. 326 - 331
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Copyright
Copyright © Cambridge University Press 2014 

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References

REFERENCES

Becker, J. A. and Speare, D. J. (2007). Transmission of the microsporidian gill parasite Loma salmonae . Animal Health Research Reviews 8, 59–68.Google Scholar
Canning, E. U. and Vavra, J. (2000). Phylum microsporidia. In The Illustrated Guide to the Protozoa, 2nd Edn. (ed. Lee, J. J., Leedale, G. F. and Bradbury, P.), pp. 39–126. Society of Protozoologists, Lawrence, KS, USA.Google Scholar
James, T. Y., Kauff, F., Schoch, C. L., Matheny, P. B., Hofstetter, V., Cox, C. J., Celio, G., Fraker, E., Miadlikowska, J., Lumbsch, H. T., Rauhut, A., Reeb, V., Arnold, A. E., Amtoft, A., Stajich, J. E., Hosaka, K., Sung, G., Johnson, D. and O'Rourke, B. (2006). Reconstructing the early evolution of fungi using a six-gene phylogeny. Nature 443, 818–822.Google Scholar
Leitch, G. J., Shaw, A. P., Colden-Stanfield, M., Scanlon, M. and Visvesvara, G. S. (2005). Multinucleate host cell induced by Vittaforma corneae (Microsporidia). Folia Parasitologica 52, 103–110.CrossRefGoogle ScholarPubMed
Lom, J. and Dyková, I. (2005). Microsporidian xenomas in fish seen in wider perspective. Folia Parasitologica 52, 69–81.Google Scholar
Lores, B., Rosales, M. J., Mascaró, C. and Osuna, A. (2003 ). In vitro culture of Glugea sp. Veterinary Parasitology 112, 185–196.Google Scholar
McConnachie, S. H., Guselle, N. J. and Speare, D. J. (2013). Retention of viable microsporidial (Loma salmonae) spores within the blue mussel (Mytilus edulis): use of an experimental laboratory model probing pathogen transfer within a multi-trophic aquaculture setting. Aquaculture 376, 1–5.CrossRefGoogle Scholar
McLeod, M. J. (2012). In vitro study of the microsporidian parasite Loma morhua, using cod-derived cells and novel culture techniques. M.Sc. thesis. University of Waterloo, Waterloo Ontario, Canada.Google Scholar
Monaghan, S. R. (2011). Use of fish cell cultures for the study and cultivation of microsporidia. Ph.D. thesis. University of Waterloo, Waterloo Ontario, Canada.Google Scholar
Monaghan, S. R., Kent, M. L., Watral, V. G., Kaufman, R. J., Lee, L. E. J. and Bols, N. C. (2009). Animal cell cultures in microsporidial research: their general roles and their specific use for fish microsporidia. In Vitro Cellular Developmental Biology – Animal 45, 135–147.Google Scholar
Shaw, R. W. and Kent, M. L. (1999) Fish microsporidia. In The Microsporidia and Microsporidiosis (ed. Wittner, M. and Weiss, L. M.), ASM Press, Washington, DC.Google Scholar
Sokolova, Y. Y., Dolgikh, V. V., Morzhina, E. V., Nassonova, E. S., Issi, I. V., Terry, R. S., Ironside, J. E., Smith, J. E. and Vossbrinck, C. R. (2003). Establishment of the genus Paranosema based on the ultrastructure and molecular phylogeny of the type species Paranosema grylli gen. nov. comb. nov. (Sokolova, Selezniov, Dolgikh, Issi 1994), from the cricket Gryllus bimaculatus Deg. Journal of Invertebrate Pathology 84, 159–172.Google Scholar
Speare, D. J. and Lovy, J. (2012). Loma salmonae and related species. In Fish Parasites: Pathobiology and Protection (ed. Woo, P. T. K. and Buchmann, K.), pp. 109–130. CAB International Oxfordshire, UK.CrossRefGoogle Scholar
Speare, D. J., Daley, J., Markham, R. J. F., Sheppard, J., Beaman, H. J. and Sanchez, J. G. (1998). Loma salmonae-associated growth rate suppression in rainbow trout Oncorhynchus mykiss (Walbaum) occurs during early onset xenoma dissolution as determined by in situ hybridization and immunohistochemistry. Journal of Fish Diseases 21, 345–354.Google Scholar
Williams, G. R., Shafer, A. B. A., Rogers, R. E. L., Shutler, D. and Stewart, D. T. (2008). First detection of Nosema ceranae, a microsporidian parasite of European honey bees (Apis mellifera), in Canada and central USA. Journal of Invertebrate Pathology 97, 189–192.CrossRefGoogle ScholarPubMed