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Detection and molecular identification of Hepatozoon canis and Babesia vogeli from domestic dogs in Palestine

Published online by Cambridge University Press:  12 December 2016

KIFAYA AZMI ,
AMER AL-JAWABREH ,
ABEDELMAJEED NASEREDDIN ,
AHMAD ABDELKADER ,
TAHER ZAID ,
SUHEIR EREQAT ,
SAMER S. SAWALHA ,
GAD BANETH  and
ZIAD ABDEEN
KIFAYA AZMI*
Affiliation:
Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, The West Bank, Palestine Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
AMER AL-JAWABREH
Affiliation:
Al-Quds Public Health Society, Jerusalem, Palestine
ABEDELMAJEED NASEREDDIN
Affiliation:
Al-Quds Public Health Society, Jerusalem, Palestine
AHMAD ABDELKADER
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
TAHER ZAID
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
SUHEIR EREQAT
Affiliation:
Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, Abu Deis, The West Bank, Palestine Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
SAMER S. SAWALHA
Affiliation:
Department of Primary Health Care, Palestinian Ministry of Health, Ramallah, Palestine
GAD BANETH
Affiliation:
Koret School of Veterinary Medicine, Hebrew University, Rehovot, Israel
ZIAD ABDEEN
Affiliation:
Faculty of Medicine, Al-Quds Nutrition and Health Research Institute, Al-Quds University, Abu-Deis, P.O. Box 20760, The West Bank, Palestine Al-Quds Public Health Society, Jerusalem, Palestine
*
*Corresponding author. Biochemistry and Molecular Biology Department, Faculty of Medicine, Al-Quds University, P.O. Box: 20002, The West Bank, Palestine. E-mail: [email protected], [email protected]
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Summary

Dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. This study aimed to observe tick borne pathogens in dogs from Palestine including Hepatozoon canis and Babesia species. The prevalence of both H. canis and Babesia species infections in apparently healthy dogs, from ten districts of the West Bank was surveyed. DNA was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). A primer set that amplifies a partial sequence of the Babesia and Hepatozoon 18S rRNA gene was used for PCR and the DNA sequences of the PCR products of all samples were determined. Twenty-nine (8·0%) of the dogs were found infected including 20 with H. canis (5·5%), seven with Babesia vogeli (1·9%) and two with undefined Babesia spp. (0·6%). Twelve Rhipicephalus sanguineus s.l ticks were pathogen-positive, including ten with H. canis (4·7%), one with B. vogeli (0·5%), and one with Hepatozoon felis (0·5%). The results indicated that a wide range of tick borne pathogens is circulating in the canine population in the surveyed region. This study is the first report on the prevalence of H. canis, B. vogeli and Babesia spp. in dogs in Palestine and its results will assist in the management of diseases associated with these blood parasites.

Keywords

canine babesiosisBabesia vogeliHepatozoan canis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 5 , April 2017 , pp. 613 - 621
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Copyright
Copyright © Cambridge University Press 2016 

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References

REFERENCES

Andersson, M. O., Bergvall, U. A., Chirico, J., Christensson, M., Lindgren, P. E., Nordstrom, J. and Kjellander, P. (2016). Molecular detection of Babesia capreoli and Babesia venatorum in wild Swedish roe deer, Capreolus capreolus . Parasites & Vectors 9, 221.Google Scholar
Azmi, K., Ereqat, S., Nasereddin, A., Al-Jawabreh, A., Baneth, G. and Abdeen, Z. (2016). Molecular detection of Theileria, Babesia, and Hepatozoon spp. in ixodid ticks from Palestine. Ticks and Tick-borne Diseases 7, 734–741.Google Scholar
Baneth, G. (2011). Perspectives on canine and feline hepatozoonosis. Veterinary Parasitology 181, 3–11.Google Scholar
Baneth, G., Samish, M. and Shkap, V. (2007). Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). Journal of Parasitology 93, 283–299.Google Scholar
Baneth, G., Sheiner, A., Eyal, O., Hahn, S., Beaufils, J. P., Anug, Y. and Talmi-Frank, D. (2013). Redescription of Hepatozoon felis (Apicomplexa: Hepatozoidae) based on phylogenetic analysis, tissue and blood form morphology, and possible transplacental transmission. Parasites & Vectors 6, 102.CrossRefGoogle ScholarPubMed
Boozer, A. L. and Macintire, D. K. (2003). Canine babesiosis. Veterinary Clinics of North America. Small Animal Practice 33, 885–904.Google Scholar
Casati, S., Sager, H., Gern, L. and Piffaretti, J. C. (2006). Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland. Annals of Agricultural and Environmental Medicine 13, 65–70.Google Scholar
Criado-Fornelio, A., Martinez-Marcos, A., Buling-Sarana, A. and Barba-Carretero, J. C. (2003). Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Part II. Phylogenetic analysis and evolutionary history. Veterinary Parasitology 114, 173–194.CrossRefGoogle ScholarPubMed
Criado-Fornelio, A., Rey-Valeiron, C., Buling, A., Barba-Carretero, J. C., Jefferies, R. and Irwin, P. (2007). New advances in molecular epizootiology of canine hematic protozoa from Venezuela, Thailand and Spain. Veterinary Parasitology 144, 261–269.Google Scholar
East, M. L., Wibbelt, G., Lieckfeldt, D., Ludwig, A., Goller, K., Wilhelm, K., Schares, G., Thierer, D. and Hofer, H. (2008). A Hepatozoon species genetically distinct from H. canis infecting spotted hyenas in the Serengeti ecosystem, Tanzania. Journal of Wildlife Diseases 44, 45–52.Google Scholar
Ereqat, S., Nasereddin, A., Al-Jawabreh, A., Azmi, K., Harrus, S., Mumcuoglu, K., Apanaskevich, D. and Abdeen, Z. (2016). Molecular detection and identification of spotted fever group Rickettsiae in ticks collected from the West Bank, Palestinian territories. PLoS Neglected Tropical Diseases 10, e0004348.CrossRefGoogle ScholarPubMed
Feldman-Muhsam, B. (1951). A note on East Mediterranean species of the Haemaphysalis . Bulletin of the Research Council of Israel 1, 96–107.Google Scholar
Feldman-Muhsam, B. (1954). Revision of the genus Hyalomma . Bulletin of the Research Council of Israel 64, 70–150.Google Scholar
Giannitti, F., Diab, S. S., Uzal, F. A., Fresneda, K., Rossi, D., Talmi-Frank, D. and Baneth, G. (2012). Infection with a Hepatozoon sp. closely related to Hepatozoon felis in a wild Pampas gray fox (Lycalopex -Pseudalopex -gymnocercus) co-infected with canine distemper virus. Veterinary Parasitology 186, 497–502.Google Scholar
Ionita, M., Mitrea, I. L., Pfister, K., Hamel, D., Buzatu, C. M. and Silaghi, C. (2012). Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach. Parasitology Research 110, 1659–1664.Google Scholar
Karagenc, T. I., Pasa, S., Kirli, G., Hosgor, M., Bilgic, H. B., Ozon, Y. H., Atasoy, A. and Eren, H. (2006). A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey. Veterinary Parasitology 135, 113–119.CrossRefGoogle ScholarPubMed
Karlsson, M. E. and Andersson, M. O. (2016). Babesia species in questing Ixodes ricinus, Sweden. Ticks and Tick-borne Diseases 7, 10–12.CrossRefGoogle ScholarPubMed
Kjemtrup, A. M. and Conrad, P. A. (2006). A review of the small canine piroplasms from California: Babesia conradae in the literature. Veterinary Parasitology 138, 112–117.Google Scholar
Kjemtrup, A. M., Wainwright, K., Miller, M., Penzhorn, B. L. and Carreno, R. A. (2006). Babesia conradae, sp. Nov., a small canine Babesia identified in California. Veterinary Parasitology 138, 103–111.CrossRefGoogle Scholar
Kubo, M., Miyoshi, N. and Yasuda, N. (2006). Hepatozoonosis in two species of Japanese wild cat. Journal of Veterinary Medical Science 68, 833–837.CrossRefGoogle ScholarPubMed
Kubo, M., Jeong, A., Kim, S. I., Kim, Y. J., Lee, H., Kimura, J., Agatsuma, T., Sakai, H. and Yanai, T. (2010). The first report of Hepatozoon species infection in leopard cats (Prionailurus bengalensis) in Korea. Journal of Parasitology 96, 437–439.CrossRefGoogle ScholarPubMed
Latrofa, M. S., Dantas-Torres, F., Giannelli, A. and Otranto, D. (2014). Molecular detection of tick-borne pathogens in Rhipicephalus sanguineus group ticks. Ticks and Tick-borne Diseases 5, 943–946.Google Scholar
Lobetti, R. G. (1998). Hyperreninaemic hypoaldosteronism in a dog. Journal of the South African Veterinary Association 69, 33–35.Google Scholar
Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M. S., Stanneck, D., Decaprariis, D., Capelli, G. and Baneth, G. (2011). Diagnosis of Hepatozoon canis in young dogs by cytology and PCR. Parasites & Vectors 4, 55.Google Scholar
Passos, L. M., Geiger, S. M., Ribeiro, M. F., Pfister, K. and Zahler-Rinder, M. (2005). First molecular detection of Babesia vogeli in dogs from Brazil. Veterinary Parasitology 127, 81–85.Google Scholar
Schetters, T. (2005 a). Diagnosis of babesiosis in the dog. Tijdschrift voor diergeneeskunde 130, 421.Google ScholarPubMed
Schetters, T. (2005 b). Vaccination against canine babesiosis. Trends in Parasitology 21, 179–184.CrossRefGoogle ScholarPubMed
Schnittger, L., Rodriguez, A. E., Florin-Christensen, M. and Morrison, D. A. (2012). Babesia: a world emerging. Infection, Genetics and Evolution: Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases 12, 1788–1809.Google Scholar
Shaw, S. E., Day, M. J., Birtles, R. J. and Breitschwerdt, E. B. (2001). Tick-borne infectious diseases of dogs. Trends in Parasitology 17, 74–80.Google Scholar
Smith, T. G. (1996). The genus Hepatozoon (Apicomplexa: Adeleina). Journal of Parasitology 82, 565–585.CrossRefGoogle ScholarPubMed
Solano-Gallego, L., Sainz, A., Roura, X., Estrada-Pena, A. and Miro, G. (2016). A review of canine babesiosis: the European perspective. Parasites & Vectors 9, 336.Google Scholar
Uilenberg, G. (2006). Babesia – a historical overview. Veterinary Parasitology 138, 3–10.Google Scholar