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Curupira-1 and Curupira-2, two novel Mutator-like DNA transposons from the genomes of human parasites Schistosoma mansoni and Schistosoma japonicum

Published online by Cambridge University Press:  15 July 2011

DANIELE S. JACINTO ,
HELOISA DOS SANTOS MUNIZ ,
THIAGO M. VENANCIO ,
R. ALAN WILSON ,
SERGIO VERJOVSKI-ALMEIDA  and
RICARDO DEMARCO
DANIELE S. JACINTO
Affiliation:
Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, 13566-590 São Carlos, SP, Brazil
HELOISA DOS SANTOS MUNIZ
Affiliation:
Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, 13566-590 São Carlos, SP, Brazil
THIAGO M. VENANCIO
Affiliation:
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil
R. ALAN WILSON
Affiliation:
Centre for Immunology and Infection, Department of Biology, University of York, York YO10 5DD, UK
SERGIO VERJOVSKI-ALMEIDA
Affiliation:
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil
RICARDO DEMARCO*
Affiliation:
Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, 13566-590 São Carlos, SP, Brazil
*
*Corresponding author: Ricardo DeMarco. Departamento de Física e Informática, Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador São Carlense, 400, 13566-590 São Carlos, SP, Brazil. Tel: +55 16 3373 8789. Fax: +55 16 3371 5381. E-mail: [email protected]
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Summary

Transposons of the Mutator superfamily have been widely described in plants, but only recently have metazoan organisms been shown to harbour them. In this work we describe novel Mutator superfamily transposons from the genomes of the human parasites Schistosoma mansoni and S. japonicum, which we name Curupira-1 and Curupira-2. Curupira elements do not have Terminal Inverted Repeats (TIRs) at their extremities and generate Target Site Duplications (TSDs) of 9 base pairs. Curupira-2 transposons code for a conserved transposase and SWIM zinc finger domains, while Curupira-1 elements comprise these same domains plus a WRKY zinc finger. Alignment of transcript sequences from both elements back to the genomes indicates that they are subject to splicing to produce mature transcripts. Phylogenetic analyses indicate that these transposons represent a new lineage of metazoan Mutator-like elements with characteristics that are distinct from the recently described Phantom elements. Description of these novel schistosome transposons provides new insights in the evolution of transposable elements in schistosomes.

Keywords

DNA transposonsplatyhelminthsrepetitive elementsgenome
Type
Research Article
Information
Parasitology , Volume 138 , Issue 9 , August 2011 , pp. 1124 - 1133
Copyright
Copyright © Cambridge University Press 2011

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