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Culture characteristics of Trypanosoma catostomi and Trypanosoma phaleri from North American freshwater fishes

Published online by Cambridge University Press:  06 April 2009

S. R. M. Jones  and
P. T. K. Woo
S. R. M. Jones
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
P. T. K. Woo
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Abstract

Culture characteristics of two species of piscine trypanosomes were compared. Trypanosoma catostomi from Catostomus commersoni was isolated in hypo-osmotic blood agar media but not in hyper-osmotic media. However, T. phaleri from Amia calva was isolated in both media. Only T. catostomi was stimulated to divide by diluting infected blood with water. The maximum number of T. phaleri and percentage of trypomastigotes were not affected by NaCl concentration. The maximum number of T. catostomi was inversely related to osmotic pressure and the percentage of trypomastigotes was directly related to osmotic pressure. This was confirmed by using equi-osmotic concentrations of NaCl, NaBr, Na acetate, KCl, choline-Cl or sucrose. Supplementation with urea had no effect on the number or morphology of trypanosomes. The survival of T. catostomi following cryopreservation was enhanced by prior incubation in hyper-osmotic media. Differential culture characteristics of these species may reflect adaptation to different host–vector systems.

Keywords

Trypanosoma catostomiTrypanosoma phalericulturemorphologyosmotic pressure
Type
Research Article
Information
Parasitology , Volume 103 , Issue 2 , October 1991 , pp. 237 - 243
Copyright
Copyright © Cambridge University Press 1991

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References

Andrade, C. R. & Andrade, P. P. (1988). Cell volume regulation in the trypanosomatid Herpetomonas samuelpessoai. Brazilian Journal of Medical and Biological Research 21, 379–84.Google ScholarPubMed
Barrow, J. H. (1953). The biology of Trypanosoma diemyctyli (Tobey). I. Trypanosoma diemyctyli in the leech, Batrachobdella picta (Verrill). Transactions of the American Microscopical Society 72, 197–216.CrossRefGoogle Scholar
Brooker, B. E. (1971). Flagellar attachment and detachment of Crithidia fasciculata to the gut wall of Anopheles gambiae. Protoplasma 73, 191–202.CrossRefGoogle Scholar
Castellani, A. & Willey, A. (1905). Observations on haematozoa in Ceylon. Quarterly Journal of Microscopic Science 49, 383–402.Google Scholar
Castellani, O., Ribeiro, L. V. & Fernandes, J. F. (1967). Differentiation of Trypanosoma cruzi in culture. Journal of Protozoology 14, 447–51.CrossRefGoogle ScholarPubMed
Clark, T. B. (1959). Comparative morphology of four genera of trypanosomatidae. Journal of Protozoology 6, 227–32.CrossRefGoogle Scholar
Cunningham, I. (1986). Infectivity of Trypanosoma rhodesiense cultivated at 28°C with various tsetse fly tissues. Journal of Protozoology 33, 226–31.CrossRefGoogle Scholar
Daly, J. J. & Degiusti, D. L. (1971). Trypanosoma catostomi n. sp. from the white sucker Catostomus c. commersoni (Lacépède). Journal of Protozoology 18, 414–17.CrossRefGoogle Scholar
Da Silva, J. B. T. & Roitman, I. (1982). Effect of temperature and osmolarity on growth of Crithidia fasciculata, C. hutneri, C. luciliae thermophila and Herpetomonas samuelpessoai. Journal of Protozoology 29, 269–72.CrossRefGoogle Scholar
De Almeida, D. F. & De Souza, W. (1978). Morphological changes of Herpetomonas samuelpessoai. Journal of Parasitology 64, 17–22.CrossRefGoogle ScholarPubMed
Deane, M. P. & Kirchner, E. (1963). Life-cycle of Trypanosoma conorhini. Influence of temperature and other factors on growth and morphogenesis. Journal of Protozoology 10, 391–400.CrossRefGoogle ScholarPubMed
Diamond, L. S. (1965). Studies on the morphology, biology and taxonomy of the trypanosomes of anura. Wildlife Diseases 44, 1–77.Google ScholarPubMed
Gradoni, L., Grammicia, M., Maroli, M., Pozio, E. & Bettini, S. (1983). A contribution to the technique of cloning Leishmania. Annales de Parasitologie Humaine et Comparée 58, 301–3.CrossRefGoogle Scholar
Hoare, C. A. (1931). Studies on Trypanosoma grayi III. Life-cycle in the tsetse-fly and in the crocodile. Parasitology 23, 449–84.CrossRefGoogle Scholar
Jones, S. R. M. & Woo, P. T. K. (1990). The biology of Trypanosoma phaleri n. sp. from bowfin, Amia calva L. in Canada and the United States. Canadian Journal of Zoology 68, 1956–61.CrossRefGoogle Scholar
Khan, R. A. (1976). The life cycle of Trypanosoma murmanensis Nikitin. Canadian Journal of Zoology 54, 1840–9.CrossRefGoogle ScholarPubMed
Letch, C. A. (1980). The life-cycle of Trypanosoma cobitis Mitrophanow 1883. Parasitology 80, 163–9.CrossRefGoogle Scholar
Ponselle, A. (1913). Researches sur la culture du trypanosomes de l'Anguille. Une nouvelle modification au milieu de Novy et MacNeal. Comptes Rendu des Séances de la Société de Biologie 74, 522–4.Google Scholar
Ponselle, A. (1923). La culture de Trypanosoma inopinatum, trypanosome pathogène de la grenouille. Annales de Parasitologie Humaine et Comparée 1, 155–8.CrossRefGoogle Scholar
Robertson, M. (1911). Transmission of flagellates living in the blood of certain freshwater fishes. Philosophical Transactions of the Royal Society of London, B 202, 29–50.Google Scholar
Steinert, M. (1958). Action morphogenetique de l'urée sur le trypanosome. Experimental Cell Research 15, 431–3.CrossRefGoogle Scholar
Tanabe, M. (1924). Studies on the haemoflagellata of the loach, Misgurnus anguillicaudatus. Kitasato Archives of Experimental Medicine 6, 121–38.Google Scholar
Taylor, A. E. R. & Baker, J. R. (1987). In Vitro Methods for Parasite Cultivation. London: Academic Press.Google Scholar
Wedemeyer, G. A. & Yasutake, W. T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health. Technical Paper of the U.S. Fish and Wildlife Service.Google Scholar
Woo, P. T. K. (1969 a). The haematocrit centrifuge for the detection of trypanosomes. Canadian Journal of Zoology 47, 921–3.CrossRefGoogle ScholarPubMed
Woo, P. T. K. (1969 b). The life cycle of Trypanosoma chrysemydis. Canadian Journal of Zoology 47, 1139–51.CrossRefGoogle Scholar
Woo, P. T. K. (1983). Sensitivity of diagnostic techniques in determining the prevalence of anuran trypanosomes. Journal of Wildlife Diseases 19, 24–6.CrossRefGoogle ScholarPubMed
Woo, P. T. K. & Black, G. A. (1984). Trypanosoma danilewskyi, host specificity and host's effect on morphometrics. Journal of Parasitology 70, 788–93.CrossRefGoogle ScholarPubMed