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Cross-stress tolerance and expression of stress-related proteins in osmotically desiccated entomopathogenic Steinernema feltiae IS-6

Published online by Cambridge University Press:  06 July 2005

S. CHEN ,
N. GOLLOP  and
I. GLAZER
S. CHEN
Affiliation:
Department of Nematology, ARO, The Volcani Center, Bet Dagan 50250, Israel
N. GOLLOP
Affiliation:
Department of Food Science, ARO, The Volcani Center, Bet Dagan 50250, Israel
I. GLAZER
Affiliation:
Department of Nematology, ARO, The Volcani Center, Bet Dagan 50250, Israel
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Abstract

Infective juveniles (IJs) of the entomopathogenic nematode (EPN) Steinernema feltiae IS-6 can survive exposure to 24% glycerol solution by entering an osmotically desiccated state. Exposure of osmotically desiccated nematodes to extreme temperature assays (40 °C for 10 h and −20 °C for 360 h) resulted in gradual reduction in survival, whereas non-desiccated IJs died within a short exposure to the assay conditions. Through SDS-PAGE, a stress-related protein UNC-87 was found in osmotically desiccated IJs exposed to 40 °C for 3, 6, and 8 h, whose survival rates were 98·9±1·43, 78·5±5·87 and 20·9±4·93%, respectively. The protein was not found in IJs following exposure of osmotically desiccated individuals to 40 °C for 10 h, in which none of the IJs survived. After exposure to −20 °C for 360 h, the survival of osmotically desiccated EPNs with a weak band of UNC-87 was 13·0±3·32%. To identify other responsive proteins that are required for osmotic stress, we used 2-dimensional electrophoresis to analyse the proteins in osmotically desiccated EPNs. The results revealed that 10 novel protein spots and 10 up-regulated protein spots in osmotically desiccated IJs were detected by digital image analysis. Mass spectrometry analysis of 7 significant spots indicated that osmotic stress in desiccated IJs was associated with the induction of actin, Proteasome regulatory particle (ATPase-like), GroEL chaperonin, GroES co-chaperonin and transposase family member. It seems to show actin, UNC-87 and Proteasome regulatory particle may play distinct roles in specific aspects of organization of macromolecular structures under desiccation stress. GroEL and GroES are members of the Hsp60 family of chaperons.

Keywords

Sternernema feltiae IS-6osmotic desiccationstress tolerance2-dimensional electrophoresisstress-related proteins
Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 695 - 703
Copyright
© 2005 Cambridge University Press

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