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Cross-fertilization as a reproductive strategy in a tissue flukes Didymosulcus katsuwonicola (Platyhelmintes: Didymozoidae) inferred by genetic analysis

Published online by Cambridge University Press:  03 July 2015

IVONA MLADINEO ,
MARINA TOMAŠ  and
RINO STANIĆ
IVONA MLADINEO*
Affiliation:
Institute of Oceanography & Fisheries, 21000 Split, Croatia
MARINA TOMAŠ
Affiliation:
Institute of Oceanography & Fisheries, 21000 Split, Croatia
RINO STANIĆ
Affiliation:
Sardina doo, Milna, Island of Brač, Croatia
*
*Corresponding author. Institute of Oceanography & Fisheries, 21000 Split, Croatia. E-mail: [email protected]
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Summary

Mitochondrial DNA locus cytochrome oxidase I was used to asses intraspecific genetic diversity of a didymozoid species Didymosulcus katsuwonicola. Adult forms of this species live encapsulated in pairs in the gills of the reared Atlantic bluefin tuna (Thunnus thynnus). The life cycle of this food-borne parasites and its migration in the host tissues after releasing from the digestive tract to the definitive site in the gills are unknown. Our goal was to assess whether two encysted didymozoids share the same haplotype, indicative of a common maternal origin, as well as the extent of cross- in respect to self-fertilization strategy. Intraspecific comparison showed high haplotype diversity, while the presence of two matching haplotypes within a single cyst encompassed only 17% of sampled individuals. This infers that cross-fertilization between paired individuals within the cyst is more common mechanism than self-fertilization. Such hermaphroditic parasite's trait suggests the existence of intricate infection and reproduction mechanisms, presumably as an adaptation for successful fulfillment of their indirect life cycle through dissemination of genetically more diverse and consequently more fit offspring.

Keywords

Bluefin tunaDidymosulcus katsuwonicolaDigeneaThunnus thynnus
Type
Research Article
Information
Parasitology , Volume 142 , Issue 11 , September 2015 , pp. 1422 - 1429
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Copyright
Copyright © Cambridge University Press 2015 

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