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Conserved peptide sequences bind to actin and enolase on the surface of Plasmodium berghei ookinetes

Published online by Cambridge University Press:  05 August 2011

J. HERNÁNDEZ-ROMANO ,
M. H. RODRÍGUEZ ,
V. PANDO ,
J. A. TORRES-MONZÓN ,
A. ALVARADO-DELGADO ,
A. N. LECONA VALERA ,
R. ARGOTTE RAMOS ,
J. MARTÍNEZ-BARNETCHE  and
M. C. RODRÍGUEZ
J. HERNÁNDEZ-ROMANO
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México Universidad Politécnica del Estado de Morelos, Boulevard Cuauhnáhuac No. 566, Col. Lomas del Texcal, Jiutepec, Morelos CP62550México
M. H. RODRÍGUEZ
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
V. PANDO
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
J. A. TORRES-MONZÓN
Affiliation:
Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, 4a. Av. Norte y 19 calle Poniente, Tapachula, Chiapas CP30700México
A. ALVARADO-DELGADO
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
A. N. LECONA VALERA
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
R. ARGOTTE RAMOS
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
J. MARTÍNEZ-BARNETCHE
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
M. C. RODRÍGUEZ*
Affiliation:
Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México
*
*Corresponding author: Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508México. Tel: +52 777 3293075. Fax: +52 777 3175485. E-mail: [email protected]; [email protected]
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Summary

The description of Plasmodium ookinete surface proteins and their participation in the complex process of mosquito midgut invasion is still incomplete. In this study, using phage display, a consensus peptide sequence (PWWP) was identified in phages that bound to the Plasmodium berghei ookinete surface and, in selected phages, bound to actin and enolase in overlay assays with ookinete protein extracts. Actin was localized on the surface of fresh live ookinetes by immunofluorescence and electron microscopy using specific antibodies. The overall results indicated that enolase and actin can be located on the surface of ookinetes, and suggest that they could participate in Plasmodium invasion of the mosquito midgut.

Keywords

actinookinetesmidgut invasion
Type
Research Article
Information
Parasitology , Volume 138 , Issue 11 , September 2011 , pp. 1341 - 1353
Copyright
Copyright © Cambridge University Press 2011

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