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Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

Published online by Cambridge University Press:  24 November 2021

Cecilia Gomes Barbosa ,
César Gómez-Hernández Open the ORCID record for César Gómez-Hernández [Opens in a new window] ,
Marcos Vinícius da Silva ,
Karine Rezende-Oliveira ,
Paula Tatiana Mutão Ferreira ,
Ana Carolina Morais de Oliveira ,
Chamberttan Souza Desidério ,
Fernanda Rodrigues Helmo ,
Tamires Marielem de Carvalho-Costa  and
Ingrid Ketlen Pereira Dos Santos
...Show all authors
Cecilia Gomes Barbosa
Affiliation:
Laboratory of Parasitology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
César Gómez-Hernández*
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Marcos Vinícius da Silva
Affiliation:
Laboratory of Parasitology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Karine Rezende-Oliveira
Affiliation:
Laboratory of Biomedical Sciences, Federal University of Uberlandia – Pontal Institute of Exact and Natural Sciences, Ituiutaba, MG, Brazil
Paula Tatiana Mutão Ferreira
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Ana Carolina Morais de Oliveira
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Chamberttan Souza Desidério
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Fernanda Rodrigues Helmo
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Tamires Marielem de Carvalho-Costa
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Ingrid Ketlen Pereira Dos Santos
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Lorena Kelly Alves Saraiva
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Carlo José Freire de Oliveira
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Juliana Reis Machado
Affiliation:
Department of General Pathology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Eloisa Amália Vieira Ferro
Affiliation:
Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Federal University of Uberlândia, Campus Santa Mônica, Uberlândia, MG, Brazil
Virmondes Rodrigues Jr.
Affiliation:
Laboratory of Immunology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
Luís Eduardo Ramirez
Affiliation:
Laboratory of Parasitology, Department of Immunology, Microbiology and Parasitology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil
*
Author for correspondence: César Gómez-Hernández, E-mail: [email protected]
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Abstract

Congenital transmission of Chagas disease plays an important role in endemic countries because it is not a diagnosis that is encountered frequently in prenatal care. Due to limited information regarding congenital transmission of Trypanosoma cruzi in Mexico, the present study aimed to investigate protozoan infectivity and modulation of immune responses in human placental explants infected with T. cruzi Ia Mexican strains. The Inc-5 strain showed increased infectivity and modulated IL-1β, IL-10 and TLR-4, decreasing their expression after 24 h of infection. Both strains (Inc-5 and Ninoa) stimulated the production of TNF-α and decreased IL-6 levels 96 h after infection. An important detachment of the syncytiotrophoblast caused by infection with T. cruzi was observed after 24 h of infection. In this study, ex vivo infection of human placental villi was performed to better understand interactions involving parasitic T. cruzi and human placental tissue. It was concluded that the strains of TcIa present parasitism in placental tissue, modulation of the innate immune system of the placenta, and cause intense detachment of the syncytiotrophoblast, a fact that may be more associated with abortion and premature birth events than the congenital transmission itself, justifying the low rate of this transmission mechanism by this genotype.

Keywords

Chagas diseasecongenital transmissionMéxicoT. cruzi Ia
Type
Research Article
Information
Parasitology , Volume 149 , Issue 3 , March 2022 , pp. 418 - 426
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally.

†

These senior authors contributed equally to this article.

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