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A comparison of membrane-bound enzymes of the isolated brush border plasma membranes of the cestodes Hymenolepis diminuta and H. microstoma

Published online by Cambridge University Press:  06 April 2009

P. W. Pappas  and
Elizabeth M. Narcisi
P. W. Pappas
Affiliation:
Department of Zoology, The Ohio State University, Columbus, Ohio 43210
Elizabeth M. Narcisi
Affiliation:
Department of Zoology, The Ohio State University, Columbus, Ohio 43210
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Summary

Preparations of isolated brush border plasma membrane of Hymenolepis diminuta and H. microstoma possess the following enzymatic activities: alkaline phosphohydrolase (E.C. 3.1.3.1); Type I phosphodiesterase (E.C. 3.1.4.1); ribonuclease (E.C. 3.1.4.22); adenosine triphosphatase (E.C. 3.6.1.3); and 5′-nucleotidase (E.C. 3.1.3.5). The following enzymatic activities could not be demonstrated in either membrane preparation: Type II phosphodiesterase (E.C. 3.1.4.18); cyclic adenosine-3′, 5′-monophosphate phosphodiesterase (E.C. 3.1.4.17); leucine aminopeptidase (E.C. 3.4.11.1); maltase (α-glucosidase; E.C. 3.2.1.20); and lactase (β-galactosidase; E.C. 3.2.1.23). These data generally agree with those of previous studies in which similar membrane-bound enzymes were demonstrated in intact (living) worms.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 2 , April 1982 , pp. 391 - 396
Copyright
Copyright © Cambridge University Press 1982

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References

von Brand, T.. (1979). Biochemistry and Physiology of Endoparasites, 447 pp. Amsterdam, New York, Oxford: Elsevier/North Holland Biomedical Press.Google Scholar
Case, G. S., Sinnott, M. L. & Tenu, J. P. (1973). The role of magnesium ions in β-galactosidase catalyzed hydrolyses. Studies on charge and shape of β-galactopyranosy 1-binding site. The Biochemical Journal 133, 99–104.CrossRefGoogle Scholar
Conway-Jones, P. B. & Rothman, A. H. (1978). Hymenolepis microstoma: ultrastructural localization of adenyl cyclase in the tegument. Experimental Parasitology 46, 152–6.CrossRefGoogle ScholarPubMed
Douch, P. G. C. (1968). L-leucyl-β-naphthylamidases of the cestode, Moniezia expansa, and the nematode, Ascaris suum. Comparative Biochemistry and Physiology 60B, 63–6.Google Scholar
Gamble, H. R. & Pappas, P. W. (1980). Solubilization of membrane-bound ribonuclease (RNase) and alkaline phosphatase from the isolated brush border of Hymenolepis diminuta (Cestoda). Journal of Parasitology 66, 434–8.CrossRefGoogle ScholarPubMed
Gamble, H. R. & Pappas, P. W. (1981 a). Partial characterization of ribonuclease (RNase) activity from the isolated and solubilized brush border of Hymenolepis diminuta. Journal of Parasitology 67, 372–7.CrossRefGoogle ScholarPubMed
Gamble, H. R. & Pappas, P. W. (1981 b). Type I phosphodiesterase in the isolated brush border plasma membrane of Hymenolepis diminuta. Journal of Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Jørgensen, B. B. & Jørgensen, O. B. (1967). Inhibition of barley malt α-glucosidase by tris(hydroxymethyl)aminomethane and erythritol. Biochimica et biophysica acta 146, 167–72.CrossRefGoogle ScholarPubMed
Knowles, W. J. & Oaks, J. A. (1979). Isolation and partial biochemical characterization of the brush border plasma membrane of the cestode Hymenolepis diminuta. Journal of Parasitology 65, 715–31.CrossRefGoogle ScholarPubMed
Lumsden, R. D., Gonzalez, G., Mills, R. R. & Viles, J. M. (1968). Cytological studies on the absorptive surfaces of cestodes, III. Hydrolysis of phosphate esters. Journal of Parasitology 54, 524–35.CrossRefGoogle Scholar
Markwell, M. A. K., Haas, S. M., Bieber, L. L. & Tolbert, N. E. (1978). A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87, 206–10.CrossRefGoogle ScholarPubMed
Moczon, T. (1974). Histochemical studies on the enzymes of Hymenolepis diminuta (Rud., 1819) (Cestoda). IV. Non-specific and specific phosphatases in a mature parasite. Acta Parasitologica Polonica 22, 323–9.Google Scholar
Pappas, P. W. (1980 a). Enzyme interactions at the host–parasite interface. In Cellular Interactions in Symbiosis and Parasitism (ed. Cook, C. B., Pappas, P. W. and Rudolph, E. D.), pp. 145–172. Columbus: The Ohio State University Press.Google Scholar
Pappas, P. W. (1980 b). Phosphohydrolase activity of the isolated brush border membrane of Hymenolepis diminuta (Cestoda) following sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Journal of Parasitology 66, 914–19.CrossRefGoogle ScholarPubMed
Pappas, P. W. (1980 c). Structure, function and biochemistry of the cestode tegumentary membrane and associated glycocalyx. In Endocytobiology (ed. Schwemmler, W. and Schenk, H. E. A.), pp. 587–603. Berlin and New York: Walter de Gruyter & Co.Google Scholar
Pappas, P. W. (1981 a). Hymenolepis diminuta: partial characterization of membrane-bound nucleotidase activities (ATPase and 5′-nucleotidase) in the isolated brush border membrane. Experimental Parasitology 51, 209–19.CrossRefGoogle ScholarPubMed
Pappas, P. W. (1981 b). Hymenolepis diminuta: partial characterization of the membrane-bound and solubilized alkaline phosphohydrolase activities of the isolated brush border plasma membrane. Experimental Parasitology 51, 209–19.CrossRefGoogle Scholar
Pappas, P. W. (1981 c). Solubilization of the membrane-bound enzymes of the brush border plasma membrane of Hymenolepis diminuta using non-ionic detergents. Journal of Parasitology (in the Press).Google Scholar
Pappas, P. W., Uglem, G. L. & Read, C. P. (1973). Ribonuclease activity associated with intact Hymenolepis diminuta. Journal of Parasitology 59, 824–8.CrossRefGoogle ScholarPubMed
Read, C. P. (1959). The role of carbohydrates in the biology of cestodes. VIII. Some conclusions and hypotheses. Experimental Parasitology 8, 365–82.CrossRefGoogle ScholarPubMed
Thompson, W. J., Terasaki, W. L., Epstein, P. M. & Strada, S. J. (1979). Assay of cyclic nucleotide phosphodiesterase and resolution of multiple molecular forms of the enzyme. In Advances in Cyclic Nucleotide Research, vol. 10 (ed. Brooker, G., Greengard, P. and Robinson, G. A.), pp. 69–92. New York: Raven Press.Google Scholar
Uglem, G. L., Pappas, P. W. & Read, C. P. (1973). Surface aminopeptidase in Moniliformis dubius and its relation to amino acid uptake. Parasitology 67, 185–95.CrossRefGoogle ScholarPubMed