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Comparison of glycolysis and glutaminolysis in Onchocerca volvulus and Brugia pahangi by 13C nuclear magnetic resonance spectroscopy

Published online by Cambridge University Press:  06 April 2009

N. E. MacKenzie ,
E. A. Van De Waa ,
P. R. Gooley ,
J. F. Williams ,
J. L. Bennett ,
S. M. Bjorge ,
T. A. Baille  and
T. G. Geary
N. E. MacKenzie
Affiliation:
Department of Pharmaceutical Sciences, University of Arizona, Tucson, AZ 85721
E. A. Van De Waa
Affiliation:
Department of Microbiology and Public Health and Department of Medicinal Chemistry, University of Washington, Seattle, WA 98185
P. R. Gooley
Affiliation:
Department of Pharmaceutical Sciences, University of Arizona, Tucson, AZ 85721
J. F. Williams
Affiliation:
Department of Microbiology and Public Health and
J. L. Bennett
Affiliation:
Department of Pharmacology and Toxicolgy, Michigan State University, East Lansing, MI 48823
S. M. Bjorge
Affiliation:
Department of Medicinal Chemistry, University of Washington, Seattle, WA 98185
T. A. Baille
Affiliation:
Department of Medicinal Chemistry, University of Washington, Seattle, WA 98185
T. G. Geary
Affiliation:
The Upjohn Company, Parasitology Research, Kalamazoo, MI 49001
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Summary

Comparison of glycolysis in Brugia pahangi and Onchocerca volvulus by C nuclear magnetic resonance (NMR) spectroscopy showed that the former organism is predominantly a lactate fermenter and the latter resembles more closely the metabolism of a mixed acid fermenter producing lactate, succinate, acetate, ethanol, formate and carbon dioxide. Both organisms synthesize glycogen as a storage carbohydrate. Glutaminolysis in both organisms proceeds by the δ-aminobutyrate shunt to produce succinate which is then further metabolized to acetate and carbon dioxide as end-products.

Keywords

Onchocerca volvulusBrugia pahangiglycolysisglutaminolysisnuclear magnetic resonance spectroscopy.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 427 - 435
Copyright
Copyright © Cambridge University Press 1989

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