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Comparison of coprological, immunological and molecular methods for the detection of dogs infected with Angiostrongylus vasorum before and after anthelmintic treatment

Published online by Cambridge University Press:  03 June 2015

M. SCHNYDER ,
R. JEFFERIES ,
A. SCHUCAN ,
E. R. MORGAN  and
P. DEPLAZES
M. SCHNYDER*
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland
R. JEFFERIES
Affiliation:
School of Veterinary Science, University of Bristol, Langford, BS40 5DU, UK School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK
A. SCHUCAN
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland
E. R. MORGAN
Affiliation:
School of Veterinary Science, University of Bristol, Langford, BS40 5DU, UK School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK
P. DEPLAZES
Affiliation:
Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, 8057 Zurich, Switzerland
*
* Corresponding author. Vetsuisse Faculty, Institute of Parasitology, University of Zurich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland. E-mail: [email protected]
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Summary

Timely diagnosis of the nematode Angiostrongylus vasorum in dogs is important in view of severe and permanent lung and cardiovascular lesions that may occur. The performance of the classical Baermann coprological method was compared with ELISAs for the serological detection of circulating antigen and specific antibodies and with Polymerase chain reaction (PCR) performed on EDTA blood, feces and tracheal swabs of serial samples from experimentally inoculated dogs over 13 weeks post inoculation (wpi) (n = 16) and following anthelmintic treatment (n = 6). Patency was observed from 6·7 to 7·6 wpi in all dogs, Baermann results were then mostly positive (116/119, 97%) during the patent period, with wide variations in the numbers of first stage larvae numbers. Blood PCR was tested positive on 1–2 occasions in 11/16 dogs in the pre-patent period, while all tested positive by antibody-detection ELISA by 6 wpi. The proportion of dogs testing positive by fecal PCR and antigen-detection ELISA rose early in the patent period. Tracheal swabs were occasionally DNA-positive in 3/16 dogs starting from 10 wpi. Following treatment, larval excretion stopped within 3 weeks and blood PCR results became negative within 1 week (5/6 dogs), while 4/6 dogs were positive for parasite DNA in tracheal swabs. Parasite antigen and specific antibodies both persisted in the blood for 3–9 weeks after treatment, with average optical densities and the proportion of positive dogs falling gradually, while results using other tests were much more variable. Results indicate that the earliest and most consistent results are obtained by the ELISAs, which can also be used for monitoring dogs after anthelmintic treatment.

Keywords

Angiostrongylus vasorumdogcopromicroscopyserologyantigen detectionantibody detectionPCRtracheal swabsfecal samplesblood
Type
Research Article
Information
Parasitology , Volume 142 , Issue 10 , September 2015 , pp. 1270 - 1277
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Copyright
Copyright © Cambridge University Press 2015 

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References

REFERENCES

Al-Sabi, M. N., Deplazes, P., Webster, P., Willesen, J. L., Davidson, R. K. and Kapel, C. M. (2010). PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes. Parasitology Research 107, 135–140.Google Scholar
Barutzki, D. and Schaper, R. (2009). Natural infections of Angiostrongylus vasorum and Crenosoma vulpis in dogs in Germany (2007–2009). Parasitology Research 105(Suppl. 1), 39–48.Google Scholar
Conboy, G. (2011). Canine angiostrongylosis: the French heartworm: an emerging threat in North America. Veterinary Parasitology 176, 382–389.Google Scholar
Cury, M. C. and Lima, W. S. (1996). Rupture of femoral artery in a dog infected with Angiostrongylus vasorum . Veterinary Parasitology 65, 313–315.CrossRefGoogle Scholar
Deplazes, P., Eckert, J., v. Samson-Himmelstjerna, G. and Zahner, H. (2013). Lehrbuch der Parasitologie für die Tiermedizin. Enke Verlag, Stuttgart.Google Scholar
Ferdushy, T., Kapel, C. M., Webster, P., Al-Sabi, M. N. and Gronvold, J. (2009). The occurrence of Angiostrongylus vasorum in terrestrial slugs from forests and parks in the Copenhagen area, Denmark. Journal of Helminthology 83, 379–383.Google Scholar
Guardone, L., Schnyder, M., Macchioni, F., Deplazes, P. and Magi, M. (2013). Serological detection of circulating Angiostrongylus vasorum antigen and specific antibodies in dogs from central and northern Italy. Veterinary Parasitology 192, 192–198.CrossRefGoogle ScholarPubMed
Guilhon, J. (1963). Recherches sur le cycle évolutif du Strongle des vaisseaux du chien. Bulletin de l’ Académie Vétérinaire 36, 431–442.CrossRefGoogle Scholar
Humm, K. and Adamantos, S. (2010). Is evaluation of a faecal smear a useful technique in the diagnosis of canine pulmonary angiostrongylosis? Journal of Small Animal Practice 51, 200–203.Google Scholar
Jefferies, R., Morgan, E. R. and Shaw, S. E. (2009). A SYBR green real-time PCR assay for the detection of the nematode Angiostrongylus vasorum in definitive and intermediate hosts. Veterinary Parasitology 166, 112–118.CrossRefGoogle ScholarPubMed
Jefferies, R., Morgan, E. R., Helm, J., Robinson, M. and Shaw, S. E. (2011). Improved detection of canine Angiostrongylus vasorum infection using real-time PCR and indirect ELISA. Parasitology Research 109, 1577–1583.CrossRefGoogle ScholarPubMed
King, M. C. A., Grose, R. M. R. and Startup, G. (1994). Angiostrongylus vasorum in the anterior chamber of a dog's eye. Journal of Small Animal Practice 35, 326–328.CrossRefGoogle Scholar
Kistler, W. M., Brown, J. D., Allison, A. B., Nemeth, N. M. and Yabsley, M. J. (2014). First report of Angiostrongylus vasorum and Hepatozoon from a red fox (Vulpes vulpes) from West Virginia, USA. Veterinay Parasitology 200, 216–220.CrossRefGoogle ScholarPubMed
Koch, J. and Willesen, J. L. (2009). Canine pulmonary angiostrongylosis: an update. Veterinary Journal 179, 348–359.Google Scholar
Matos, J. M., Schnyder, M., Bektas, R., Makara, M., Kutter, A., Jenni, S., Deplazes, P. and Glaus, T. (2012). Recruitment of arteriovenous pulmonary shunts may attenuate the development of pulmonary hypertension in dogs experimentally infected with Angiostrongylus vasorum . Journal of Veterinary Cardiology 14, 313–322.Google Scholar
McGarry, J. W. and Morgan, E. R. (2009). Identification of first-stage larvae of metastrongyles from dogs. Veterinary Record 165, 258–261.CrossRefGoogle ScholarPubMed
Morgan, E. and Shaw, S. (2010). Angiostrongylus vasorum infection in dogs: continuing spread and developments in diagnosis and treatment. Journal of Small Animal Practice 51, 616–621.Google Scholar
Oliveira-Junior, S. D., Barcante, J. M., Barcante, T. A., Ribeiro, V. M. and Lima, W. S. (2004). Ectopic location of adult worms and first-stage larvae of Angiostrongylus vasorum in an infected dog. Veterinary Parasitology 121, 293–296.Google Scholar
Oliveira-Junior, S. D., Barcante, J. M., Barcante, T. A., Dias, S. R. and Lima, W. S. (2006). Larval output of infected and re-infected dogs with Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905. Veterinary Parasitology 141, 101–106.CrossRefGoogle ScholarPubMed
Paradies, P., Schnyder, M., Capogna, A., Lia, R. P. and Sasanelli, M. (2013). Canine angiostrongylosis in naturally infected dogs: clinical approach and monitoring of infection after treatment. Scientific World Journal 2013, 702056.CrossRefGoogle ScholarPubMed
Schnyder, M., Fahrion, A., Ossent, P., Kohler, L., Webster, P., Heine, J. and Deplazes, P. (2009). Larvicidal effect of imidacloprid/moxidectin spot-on solution in dogs experimentally inoculated with Angiostrongylus vasorum . Veterinary Parasitolology 166, 326–332.Google Scholar
Schnyder, M., Fahrion, A., Riond, B., Ossent, P., Webster, P., Kranjc, A., Glaus, T. and Deplazes, P. (2010 a). Clinical, laboratory and pathological findings in dogs experimentally infected with Angiostrongylus vasorum . Parasitology Research 107, 1471–1480.Google Scholar
Schnyder, M., Maurelli, M. P., Morgoglione, M. E., Kohler, L., Deplazes, P., Torgerson, P., Cringoli, G. and Rinaldi, L. (2010 b). Comparison of faecal techniques including FLOTAC for copromicroscopic detection of first stage larvae of Angiostrongylus vasorum . Parasitology Research 109, 63–69.CrossRefGoogle ScholarPubMed
Schnyder, M., Tanner, I., Webster, P., Barutzki, D. and Deplazes, P. (2011). An ELISA for sensitive and specific detection of circulating antigen of Angiostrongylus vasorum in serum samples of naturally and experimentally infected dogs. Veterinary Parasitology 179, 152–158.CrossRefGoogle ScholarPubMed
Schnyder, M., Schaper, R., Bilbrough, G., Morgan, E. R. and Deplazes, P. (2013 a). Seroepidemiological survey for canine angiostrongylosis in dogs from Germany and the UK using combined detection of Angiostrongylus vasorum antigen and specific antibodies. Parasitology 140, 1442–1450.Google Scholar
Schnyder, M., Schaper, R., Pantchev, N., Kowalska, D., Szwedko, A. and Deplazes, P. (2013 b). Serological detection of circulating Angiostrongylus vasorum antigen- and parasite-specific antibodies in dogs from Poland. Parasitology Research 112(Suppl. 1), 109–117.Google Scholar
Schnyder, M., Stebler, K., Naucke, T. J., Lorentz, S. and Deplazes, P. (2014). Evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis. Parasites and Vectors 7, 72.CrossRefGoogle ScholarPubMed
Schucan, A., Schnyder, M., Tanner, I., Barutzki, D., Traversa, D. and Deplazes, P. (2012). Detection of specific antibodies in dogs infected with Angiostrongylus vasorum . Veterinary Parasitology 185, 216–224.Google Scholar
Staebler, S., Ochs, H., Steffen, F., Naegeli, F., Borel, N., Sieber-Ruckstuhl, N. and Deplazes, P. (2005). Autochthonous infections with Angiostrongylus vasorum in dogs in Switzerland and Germany (in German). Schweizer Archiv fur Tierheilkunde 147, 121–127.Google Scholar
Traversa, D., Iorio, R. and Otranto, D. (2008). Diagnostic and clinical implications of a nested PCR specific for ribosomal DNA of the feline lungworm Aelurostrongylus abstrusus (Nematoda, Strongylida). Journal of Clinical Microbiology 46, 1811–1817.Google Scholar