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Cloning of the rDNA repeat unit from a British entomopathogenic nematode (Steinernematidae) and its potential for species identification

Published online by Cambridge University Press:  06 April 2009

A. P. Reid  and
W. M. Hominick
A. P. Reid
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks SL5 7PY
W. M. Hominick
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks SL5 7PY
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Summary

The entire ribosomal DNA repeat unit of a steinernematid species (Nashes isolate) was cloned as three separate EcoR I fragments in the plasmid pUC18. An equimolar cocktail of these three clones was used to identify Steinernema species on Southern blots as each species displays its own unique restriction fragment length polymorphisms. The clones also identified two new species isolated in a soil survey of coastal regions of Britain. One of the clones (pSn4.0) can detect length heterogeneities in the rDNA repeat unit of various isolates of some of the species, particularly the most common in the United Kingdom, S. feltiae. These differences in the rDNA repeat unit length remained constant over several years for one isolate of S. feltiae, but were different for each of the geographical isolates studied to date.

Keywords

ribosomal DNARFLPSteinernema sppentomopathogenic nematodesspecies identification
Type
Research Article
Information
Parasitology , Volume 107 , Issue 5 , December 1993 , pp. 529 - 536
Copyright
Copyright © Cambridge University Press 1993

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