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A cloned kinetoplast DNA mini-circle fragment from a Leishmania spp. specific for post-kala-azar dermal leishmaniasis strains

Published online by Cambridge University Press:  06 April 2009

S. Das Gupta ,
D. K. Ghosh  and
H. K. Majumder
S. Das Gupta
Affiliation:
Leishmania Group, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India
D. K. Ghosh
Affiliation:
Leishmania Group, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India
H. K. Majumder
Affiliation:
Leishmania Group, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India
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Summary

DNA–DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.

Keywords

Leishmania spp.mini-circlehybridizationDNA probes
Type
Research Article
Information
Parasitology , Volume 102 , Issue 2 , April 1991 , pp. 187 - 191
Copyright
Copyright © Cambridge University Press 1991

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