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Circulating filarial antigen detection in brugian filariasis

Published online by Cambridge University Press:  08 December 2015

PRAVEEN KUMAR TRIPATHI ,
RAMESH CHANDER MAHAJAN ,
NANCY MALLA ,
ABHISHEK MEWARA ,
SHAILJA MISRA BHATTACHARYA ,
RANGANATHA KRISHNA SHENOY  and
RAKESH SEHGAL
PRAVEEN KUMAR TRIPATHI
Affiliation:
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
RAMESH CHANDER MAHAJAN
Affiliation:
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
NANCY MALLA
Affiliation:
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
ABHISHEK MEWARA
Affiliation:
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
SHAILJA MISRA BHATTACHARYA
Affiliation:
Division of Parasitology, Central Drug Research Institute (CDRI), Lucknow, India
RANGANATHA KRISHNA SHENOY
Affiliation:
Department of Medicine, Filariasis Chemotherapy Unit, TD Medical College Hospital, Alleppey, Kerala, India
RAKESH SEHGAL*
Affiliation:
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
*
*Corresponding author: Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh – 160012, India. Tel: 911722755168. Fax: 911722744401. E-mail: [email protected]
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Summary

Human lymphatic filariasis (LF) is a major cause of disability globally. The success of global elimination programmes for LF depends upon effectiveness of tools for diagnosis and treatment. In this study on stage-specific antigen detection in brugian filariasis, L3, adult worm (AW) and microfilarial antigenaemia were detected in around 90–95% of microfilariae carriers (MF group), 50–70% of adenolymphangitis (ADL) patients, 10–25% of chronic pathology (CP) patients and 10–15% of endemic normal (EN) controls. The sensitivity of the circulating filarial antigen (CFA) detection in serum samples from MF group was up to 95%. In sera from ADL patients, unexpectedly, less antigen reactivity was observed. In CP group all the CFA positive individuals were from CP grade I and II only and none from grade III or IV, suggesting that with chronicity the AWs lose fecundity and start to disintegrate and die. Amongst EN subject, 10–15% had CFA indicating that few of them harbour filarial AWs, thus they might not be truly immune as has been conventionally believed. The specificity for antigen detection was 100% when tested with sera from various other protozoan and non-filarial helminthic infections.

Keywords

Brugian filariasiscirculating filarial antigen (CFA)stage-specific antigenaemiapre- and post-treatment kinetics
Type
Research Article
Information
Parasitology , Volume 143 , Issue 3 , March 2016 , pp. 350 - 357
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Copyright
Copyright © Cambridge University Press 2015 

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