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Circulating antigens, antibodies and immune complexes in experimental Taenia pisiformis infections of rabbits

Published online by Cambridge University Press:  06 April 2009

P. S. Craig
P. S. Craig
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA
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Summary

Circulating antigen has been detected in the serum of rabbits experimentally infected with Taenia pisiformis. A rabbit antiserum against an antigen preparation derived from the in vitro maintenance of living metacestodes of T. pisiformis was used in a double-antibody enzyme-linked immunosorbent assay (ELISA). Antigen was detected in infected rabbit serum 48 h after infection and peak levels were recorded at 2 weeks post-infection or post-challenge. The pattern of circulating immune complex formation followed closely that of circulating antigen, and infected rabbit serum contained both anti-metacestode and anti-oncospheral antibodies. Serum antigen levels were just detectable (0·4–0·8 μg protein/ml) over the period (> 2 weeks post-infection) of peritoneal cyst development. The circulating antigen probably has antigenic determinants in common with the oncosphere, juvenile and mature metacestode stages. Possible reasons for the temporal variation in serum antigen levels are discussed.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 1 , August 1984 , pp. 121 - 132
Copyright
Copyright © Cambridge University Press 1984

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References

Ali-Khan, Z., Jothy, S. & Siboo, R. (1982). Amyloidosis in experimental murine alveolar hydatidosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 169–71.CrossRefGoogle ScholarPubMed
Au, A. C. S., Denham, D. A., Steward, M. W., Draper, C. C., Ismail, M. M., Rao, C. K. & Mak, J. W. (1981). Detection of circulating antigens and immune complexes in feline and human lymphatic filariasis. Southeast Asian Journal of Tropical Medicine and Public Health 12, 492–8.Google ScholarPubMed
Bekhti, A., Schaaps, J. P., Capron, M., Dessaint, J. P., Santoro, F. & Capron, A. (1977). Treatment of hepatic hydatid disease with mebendazole. Preliminary results in four cases. British Medical Journal 2, 1047–51.CrossRefGoogle ScholarPubMed
Berggren, W. L. & Weller, T. H. (1967). Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 16, 606–12.CrossRefGoogle ScholarPubMed
Bout, D., Santoro, F., Carlier, Y., Bina, J. C. & Capron, A. (1977). Circulating immune complexes in schistosomiasis. Immunology 33, 17–22.Google ScholarPubMed
Chemtai, A. K., Bowry, T. R. & Ahmad, Z. (1981). Evaluation of five immunodiagnostic techniques in echinococcosis patients. Bulletin of the World Health Organization 59, 767–72.Google ScholarPubMed
Coltorti, E. A. & Varela-Diaz, V. M. (1974). Echinococcus granulosus: penetration of macromolecules and their localisation on the parasite membrane of cysts. Experimental Parasitology 35, 225–31.CrossRefGoogle Scholar
Coman, B. J. & Rickard, M. D. (1975). The location of Taenia pisiformis, Taenia ovis and Taenia hydatigena in the gut of the dog and its effect on the net environmental contamination with ova. Zeitschrift für Parasitenkunde 47, 237–48.CrossRefGoogle ScholarPubMed
Craig, P. S. & Rickard, M. D. (1981 a). Studies on the specific immunodiagnosis of larval cestode infections of cattle and sheep using antigens purified by affinity chromatography in an enzyme-linked immunosorbent assay (ELISA). International Journal for Parasitology 11, 441–9.CrossRefGoogle Scholar
Craig, P. S. & Rickard, M. D. (1981 b). Anti-oncospheral antibodies in the serum of lambs experimentally infected with either Taenia ovis or Taenia hydatigena. Zeitschrift für Parasitenkunde 64, 169–77.CrossRefGoogle ScholarPubMed
Craig, P. S. & Rickard, M. D. (1982). Antibody responses of experimentally infected lambs to antigens collected during the in vitro maintenance of adult, metacestode or oncosphere stages of Taenia hydatigena and Taenia ovis with further observations on anti-oncospheral antibodies. Zeitschrift für Parasitenkunde 67, 197–209.CrossRefGoogle ScholarPubMed
Des Moutis, I., Ouaissi, A., Grzych, J. M., Yarzabal, L., Haque, A. & Capron, A. (1983). Onchocerca volvulus: detection of circulating antigen by monoclonal antibodies in human onchocerciasis. American Journal of Tropical Medicine and Hygiene 32, 533–42.CrossRefGoogle ScholarPubMed
Ellens, D. J. & Gielkins, A. L. J. (1980). A simple method for the purification of 5-amino-salicylic acid. Application of the product as substrate in enzyme-linked immunosorbent assay (ELISA). Journal of Immunological Methods 37, 325–32.CrossRefGoogle Scholar
Flisser, A., Woodhouse, E. & Larralde, C. (1980). Human cysticercosis: antigens, antibodies and non-responders. Clinical and Experimental Immunology 39, 27–37.Google ScholarPubMed
Franks, M. B. (1946). Specific soluble antigen in the blood of filarial patients. Journal of Parasitology 32, 400–6.CrossRefGoogle ScholarPubMed
Hartree, F. E. (1972). Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48, 422–7.CrossRefGoogle ScholarPubMed
Hustead, S. T. & Williams, J. F. (1977). Permeability studies of taeniid metacestodes. I. Uptake of proteins by larval stages of Taenia taeniaeformis, Taenia crassiceps and Echinococcus multilocularis. Journal of Parasitology 63, 314–21.CrossRefGoogle Scholar
Johnstone, A. & Thorpe, R. (1982). Immunochemistry in Practice. London: Blackwell Scientific Publications.Google Scholar
Karavodin, L. M. & Ash, L. R. (1980). Circulating immune complexes in experimental filariasis. Clinical and Experimental Immunology 40, 312–17.Google ScholarPubMed
Leikina, E. S., Kovrova, E. A. & Krasovskaya, N. N. (1982). Detection of circulating antigens in the bloodstream of patients with hydatid disease, alveococcosis and trichinosis. Meditsinskaya Parazitologiya in Parazitarnye Bolozni 60, 7–15.Google Scholar
Madwar, M. A. & Voller, A. (1977). Serological investigations on patients with schistosomiasis with special reference to circulating antigen. Tropenmedizin und Parasitologie 28, 57–62.Google ScholarPubMed
Musiani, P., Piantelli, M., Lauriola, L., Arru, E. & Pozzuoli, R. (1974). A solid-phase radioimmunoassay for the diagnosis of human hydatidosis. Journal of Immunology 112, 1674–9.CrossRefGoogle ScholarPubMed
Németh, I. (1971). Immunological study of rabbit cysticercosis. III. The precipitin response to experimental infection with Cysticercus pisiformis (Bloch, 1780). Acta Veterinaria Academiae Scientiarum Hungaricae 21, 319–31.Google Scholar
Pini, C., Pastore, R. & Valesini, G. (1983). Circulating immune complexes in sera of patients infected with Echinococcus granulosus. Clinical and Experimental Immunology 51, 572–8.Google ScholarPubMed
Rajasekariah, G. R., Rickard, M. D. & Mitchell, G. F. (1980). Density gradient separation of Taenia pisiformis oncospheres. Journal of Parasitology 66, 355–6.CrossRefGoogle ScholarPubMed
Richard-Lenoble, D., Smith, M. D., Loisy, M. &Verroust, P. J. (1978). Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and the detection of circulating immune complexes. Annals of Tropical Medicine and Parasitology 72, 533–60.CrossRefGoogle ScholarPubMed
Richards, S. K., Arme, C. & Bridges, J. F. (1983). Echinococcus granulosus equinus: an ultrastructural study of murine tissue response to hydatid cysts. Parasitology 86, 407–17.CrossRefGoogle ScholarPubMed
Riha, I., Haskova, J., Kalik, J., Maierova, M. & Stransky, J. (1979). The use of polyethylene glycol for immune complex detection in human sera. Molecular Immunology 16, 489–93.CrossRefGoogle ScholarPubMed
Santoro, F., Vandemeulebrouke, B. & Capron, A. (1979). Schistosoma mansoni: circulating antigens and immune complexes in infected mice. Experimental Parasitology 47, 392–402.CrossRefGoogle ScholarPubMed
Sogandares-Bernal, F., Race, M. C., Dennis, M. V. & Voge, M. (1981). Circulating antigens in infections of mice by tetrathyridia of Mesocestoides corti Hoeppli, 1925. Zeitschrift für Parasitenkunde 64, 157–67.CrossRefGoogle ScholarPubMed
Tanaka, K., Kawamura, H., Tohgi, N., Tsuji, M., Miyachi, Y. & Miyoshi, A. (1983). The measurement of Ascaris suum protein by radioimmunoassay in sera from patients with helminthiasis and with gastrointestinal diseases. Parasitology 86, 291–300.CrossRefGoogle ScholarPubMed
Voge, M., Sogandares-Bernal, F. & Martin, J. H. (1979). Fine structure of the tegument of Mesocestoides corti, tetrathyridia by scanning and transmission electron microscopy. Journal of Parasitology 65, 562–7.CrossRefGoogle ScholarPubMed
Voller, A., Bidwell, D. E. & Bartlett, A. (1976). Enzyme immunoassays in diagnostic medicine. Theory and practice. Bulletin of the World Health Organization 53, 55–65.Google ScholarPubMed
Wilson, B. M. & Nakane, P. K. (1978). Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescent and Related Staining Techniques (ed. Knapp, W., Holubar, K. and Wicks, G.), pp. 215–24. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Yong, W. K., Heath, D. D. & Parmeter, S. N. (1978). Echinococcus granulosus, Taenia hydatigena, T. ovis: evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep. New Zealand Veterinary Journal 26, 231–4.CrossRefGoogle ScholarPubMed
Zvolinskene, V. (1981). Determination of circulating hydatid antigens in the sera of patients with hydatidosis and their diagnostic significance. Acta Parasitologica Lituanica 19, 56–62.Google Scholar