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Characterization of Trichomonas vaginalis haemolysis

Published online by Cambridge University Press:  06 April 2009

D. C. Dailey ,
C. Te-Hung  and
J. F. Alderete
D. C. Dailey
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
C. Te-Hung
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
J. F. Alderete
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas, USA.
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Extract

The haemolytic activity of live Trichomonas vaginalis organisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 μm filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by > 50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis. N-μ-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.

Keywords

Trichomonas vaginalishaemolytic activitymetronidazolecysteine proteinases
Type
Research Article
Information
Parasitology , Volume 101 , Issue 2 , October 1990 , pp. 171 - 175
Copyright
Copyright © Cambridge University Press 1990

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