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Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding

Published online by Cambridge University Press:  06 April 2009

A. J. G. Simpson  and
S. R. Smithers
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

The surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificities. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetyl-glucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 1 , August 1980 , pp. 1 - 15
Copyright
Copyright © Cambridge University Press 1980

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