Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-23T02:44:50.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis

Published online by Cambridge University Press:  06 April 2009

C. A. Sutton ,
M. W. Shirley  and
M. H. Wisher
C. A. Sutton
Affiliation:
AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA
M. W. Shirley
Affiliation:
AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA
M. H. Wisher
Affiliation:
AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA
Get access Rights & Permissions [Opens in a new window]

Summary

Two dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS–PAGE) has been used to produce ‘fingerprint’ maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. lodination of sporozoites, 2D SDS–PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a surface moiety and those which were antigenic.

Keywords

coccidial proteinsEimeria spp.fingerprint maps2D SDS–PAGE
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 175 - 187
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aquadro, C. F. & Avise, J. C. (1981). Genetic divergence between rodent species assessed by using two-dimensional electrophoresis. Proceedings of the National Academy of Sciences, USA 78, 3784–8.CrossRefGoogle ScholarPubMed
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram amounts of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–54.CrossRefGoogle Scholar
Chapman, H. D. (1978). Studies on the excystation of different species of Eimeria in vitro. Zeitschrift für Parasitenkunde 56, 115–21.CrossRefGoogle ScholarPubMed
Fenton, B., Walker, A. & Walliker, D. (1985). Protein variation in clones of Plasmodium falciparum detected by two dimensional electrophoresis. Molecular and Biochemical Parasitology 16, 173–83.CrossRefGoogle ScholarPubMed
Hancock, K. & Tsang, V. C. W. (1983). India ink staining of proteins on nitrocellulose paper. Analytical Biochemistry 133, 157–62.CrossRefGoogle ScholarPubMed
Horton-smith, C. & Long, P. L. (1959). The effects of different anticoccidial agents on the intestinal coccidioses of the fowl. Journal of Comparative Pathology and Therapeutics 69, 192–207.CrossRefGoogle ScholarPubMed
Jäckle, H. (1979). Visualisation of proteins after isoelectric focusing during two-dimensional gel electrophoresis. Analytical Biochemistry 98, 81–4.CrossRefGoogle ScholarPubMed
Jeffers, T. K. (1975). Attenuation of Eimeria tenella through selection for precociousness. Journal of Parasitology 61, 1083–90.CrossRefGoogle ScholarPubMed
Langley, R. C. Jr., Cali, A. & Somberg, E. W. (1987). Two-dimensional electrophoretic analysis of spore proteins of the Microsporida. Journal of Parasitology 73, 910–18.CrossRefGoogle ScholarPubMed
Lewontin, R. C. & Hubby, J. L. (1966). A molecular approach to the study of genetic heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54, 595–609.CrossRefGoogle Scholar
Long, P. L. (1959). A study of Eimeria maxima, Tyzzer 1929 a coccidium of the fowl (Gallus gallus). Annals of Tropical Medicine and Parasitology 53, 325–33.CrossRefGoogle ScholarPubMed
Long, P. L. (1967 a). Studies on Eimeria mivati in chickens and a comparison with Eimeria acervulina. Journal of Comparative Pathology and Therapeutics 77, 315–25.CrossRefGoogle Scholar
Long, P. L. (1967b). Studies on Eimeria praecox Johnson, 1930, in the chicken. Parasitology 57, 351–61.CrossRefGoogle Scholar
Long, P. L. (1972). Eimeria tenella: reproduction, pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. Journal of Comparative Pathology and Therapeutics 82, 429–37.CrossRefGoogle ScholarPubMed
Long, P. L., Millard, B. J., Joyner, L. P. & Norton, C. C.(1976). A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Veterinaria Latina 6, 201–17.Google Scholar
McDonald, V. & Ballingall, S. (1983). Attenuation of Eimeria mivati ( = mitis) by Selection for precocious development. Parasitology 86, 371–9.CrossRefGoogle ScholarPubMed
McDonald, V., Ballingall, S. & Shirley, M. W. (1982). A preliminary study of the nature of infection and immunity in chickens given an attenuated line of Eimeria acervulina. Parasitology 84, 21–30.CrossRefGoogle ScholarPubMed
McDonald, V. & Shirley, M. W. (1987). The endogenous development of virulent strains and attenuated precocious lines of Eimeria tenella and E. necatrix. Journal of Parasitology 73, 993–7.CrossRefGoogle ScholarPubMed
McDonald, V., Shirley, M. W. & Bellatti, M. A. (1986). Eimeria maxima: characteristics of attenuated lines obtained by selection for precocious development in the chicken. Experimental Pathology 61, 192–200.Google ScholarPubMed
McDonald, V., Shirley, M. W. & Chapman, H. D. C. (1985). Attenuation of Eimeria species: further characterisation of two lines of Eimeria mitis. Research in Veterinary Science 39, 328–32.CrossRefGoogle ScholarPubMed
Merrill, C. R., Dunau, M. L. & Goldman, D. (1981). A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Analytical Biochemistry 110, 201–7.CrossRefGoogle Scholar
Merrill, C. R., Goldman, D., Sedman, S. A. & Ebert, H. M. (1981). Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211, 1437–8.CrossRefGoogle Scholar
Morrisey, J. H. (1981). Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117, 307–10.CrossRefGoogle Scholar
Oakley, B. R., Kirsch, D. R. & Morris, N. R. (1980). A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105, 361–3.CrossRefGoogle ScholarPubMed
O'Farrell, P. H. (1975). High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–21.CrossRefGoogle ScholarPubMed
O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. (1977). High resolution two-dimensional electrophoresis of basic as well as acidic proteins.Cell 12, 1133–42.CrossRefGoogle ScholarPubMed
Poehling, H. M. & Neuhoff, V. (1981). Visualization of proteins with a silver ‘stain’: A critical analysis. Electrophoresis 2, 141–7.CrossRefGoogle Scholar
Rollinson, D. (1975). Electrophoretic variation of the enzymes in chicken coccidia. Transactions of the Royal Society of Tropical Medicine and Hygiene 69, 436–7.Google Scholar
Rose, M. E. & Mockett, A. P. A. (1983). Antibodies to coccidia: detection by the enzyme-linked immunosorbent assay (ELISA). Parasite Immunology 5, 479–89.CrossRefGoogle ScholarPubMed
Ryley, J. F., Millard, B. J. & Long, P. L. (1972). Further studies on the life cycle of Eimeria brunetti Levine 1942. Zeitschrift für Parasitenkunde 40, 35–48.CrossRefGoogle ScholarPubMed
Scheele, G. A., Pash, J. & Bieger, W. (1981). Identification of proteins according to biological activity following separation by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis. Analysis of human exocrine pancreatic proteins. Analytical Biochemistry 112, 304–13.CrossRefGoogle ScholarPubMed
Shirley, M. W. (1975). Enzyme variation in Eimeria species of the chicken. Parasitology 71, 369–76.CrossRefGoogle ScholarPubMed
Shirley, M. W. (1980). Eimeria necatrix: the development of an egg adapted (attenuated) line. Parasitology 81, 525–35.CrossRefGoogle ScholarPubMed
Shirley, M. W. & Bellatti, M. A. (1984). Eimeria necatrix: selection and characteristics of a precocious (and attenuated) line. Avian Pathology 13, 657–68.CrossRefGoogle ScholarPubMed
Shirley, M. W., Jeffers, T. K. & Long, P. L. (1983). Studies to determine the taxonomic status of Eimeria mitis, Tyzzer 1929 and E. mivati, Edgar and Seibold 1964. Parasitology 87, 185–98.CrossRefGoogle Scholar
Shirley, M. W., McDonald, V. & Bellatti, M. A. (1986). Eimeria brunetti: selection and characteristics of a precocious (and attenuated) line. Avian Pathology 15, 705–17.CrossRefGoogle ScholarPubMed
Shirley, M. W., McDonald, V., Chapman, H. D. C. & Millard, B. J. (1984). Eimeria praecox: Selection and characteristics of precocious lines. Avian Pathology 13, 669–82.CrossRefGoogle ScholarPubMed
Shirley, M. W. & Rollinson, D. (1979). Coccidia: The recognition and characterization of populations of Eimeria. In Problems in the Identification of Parasites and their Vectors. Symposia of the British Society for Parasitology, vol. 17, (ed. Taylor, A. E. R & Muller, R.), pp. 7–30. Oxford: Blackwell Scientific Publications.Google Scholar
Somerville, L. L. & Wang, K. (1981). The ultrasensitive silver ‘protein’ stain also detects nanograms of nucleic acids. Biochemical and Biophysical Research Communications 102, 53–8.CrossRefGoogle ScholarPubMed
Tait, A. (1981). Analysis of protein variation in Plasmodium falciparum by two dimensional gel electrophoresis. Molecular and Biochemical Parasitology 2, 205–18.CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Tracy, R. P., Katzmann, J. A., Kimlinger, T. K., Hurst, G. A. & Young, D. S. (1983). Development of monoclonal antibodies to proteins separated by two- dimensional gel electrophoresis. Journal of Immunological Methods 65, 97–107.CrossRefGoogle ScholarPubMed
Wang, C. C. (1978). The prokaryotic characteristics of Eimeria tenella ribosomes. Comparative Biochemistry and Physiology 61B, 571–9.Google Scholar
Wang, C. D. & Stotish, R. L. (1975). Changes in nucleic acids and proteins in the oocytes of Eimeria tenella during sporulation. Journal of Protozoology 22, 438–43.CrossRefGoogle Scholar
Wisher, M. H. (1986). Identification of the sporozoite antigens of Eimeria tenella. Molecular and Biochemical Parasitology 21, 7–15.CrossRefGoogle ScholarPubMed
Wisher, M. H. & Rose, M. E. (1984). The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation. Parasitology 88, 515–19.CrossRefGoogle ScholarPubMed