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Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
O. K. Ole-Moiyoi ,
C. L. Baldwin ,
T. T. Dolan ,
C. J. O'Callaghan ,
R. E. G. Njamunggehr ,
J. G. Grootenhuis ,
D. A. Stagg ,
B. L. Leitch  and
A. S. Young
P. A. Conrad
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
O. K. Ole-Moiyoi
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
C. L. Baldwin
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
T. T. Dolan
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
C. J. O'Callaghan
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
R. E. G. Njamunggehr
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
J. G. Grootenhuis
Affiliation:
2Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya
D. A. Stagg
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
B. L. Leitch
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
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Summary

The characteristics of intra-lymphocytic Theileria isolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen with Theileria-infected lymphoblastoid cell isolates taken from the lymph nodes and blood of the same animals. Two DNA probes, selected from a genomic library of T. parva piroplasm DNA cloned in λgt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and uncloned Theileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typical T. p. lawrencei reactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them from T. p. parva and T. p. bovis parasites. The validity of these subspecies distinctions is discussed.

Keywords

Theileria parvaDNA probesbuffalo characterizationmonoclonal antibodies
Type
Research Article
Information
Parasitology , Volume 98 , Issue 2 , April 1989 , pp. 179 - 188
Copyright
Copyright © Cambridge University Press 1989

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