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Changes in cuticular permeability associated with recovery from anhydrobiosis in the plant parasitic nematode, Ditylenchus dipsaci

Published online by Cambridge University Press:  06 April 2009

D. A. Wharton ,
C. M. Preston ,
J. Barrett  and
R. N. Perry
D. A. Wharton
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
C. M. Preston
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
J. Barrett
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
R. N. Perry*
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed SY23 3DA, Wales, UK
*
3Department of Nematology and Entomology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ UK.
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Summary

The cuticular permeability of anhydrobiotic 4th-stage juveniles of Ditylenchus dipsaci decreases during rehydration, as indicated by staining with osmium tetroxide, the measurement of permeability coefficients by the rate of uptake of tritiated water from a HOH/C-inulin bathing solution and by an increased ability to slow down the rate of water loss and to survive subsequent desiccation. The initial decrease in permeability coefficient is rapid, suggesting a physical effect due to the rehydration of the cuticle, followed by a slower reduction over the subsequent 1–4 h which is dependent upon metabolic activity. The reestablishment of the permeability barrier is sensitive to metabolic inhibitors which affect enzyme activity and post-transcriptional protein synthesis. Sodium iodoacetamide was the only inhibitor which affected maintenance of the permeability barrier. An Arrhenius plot of changes in cuticular permeability with temperature indicated a sharp increase in permeability at 40–50°C and brief exposure to diethyl ether resulted in loss of the permeability barrier, suggesting that a superficial layer, probably the epicuticle, is responsible for controlling cuticular permeability.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 317 - 330
Copyright
Copyright © Cambridge University Press 1988

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Footnotes

1

Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand.

2

Department of Microbiology, University College, Newport Rd, Cardiff CF2 1TA, UK.

References

REFERENCES

Barrett, J. (1981). Biochemistry of Parasitic Helminths. London: Macmillans.CrossRefGoogle Scholar
Barrett, J. (1982). Metabolic responses to anabiosis in the fourth stage juveniles of Ditylenchus dipsaci (Nematoda). Proceedings of the Royal Society of London B 216, 159–77.Google Scholar
Bird, A. F. (1980). The nematode cuticle and its surface. In Nematodes as Biological Models, vol. 2 (ed. Zuckerman, B. M.). New York and London: Academic Press.Google Scholar
Bird, A. F. (1987). Moulting of parasitic nematodes. International Journal for Parasitology 17, 233–9.Google Scholar
Bird, A. F. & Buttrose, M. S. (1974). Ultrastructural changes in the nematode Anguina tritici associated with anhydrobiosis. Journal of Ultrastructural Research 48, 177–89.Google Scholar
Bird, A. F. & McClure, M. A. (1976). The tylenchid (Nematoda) egg shell: structure, composition and permeability. Parasitology 72, 19–28.Google Scholar
Christie, W. W. (1982). Lipid Analysis, 2nd edn. Oxford: Pergamon Press.Google Scholar
Crowe, J. H., O'Dell, S. J. & Armstrong, D. A. (1979). Anhydrobiosis in nematodes: permeability during rehydration. Journal of Experimental Zoology 207, 431–8.Google Scholar
Davey, K. G. & Rogers, W. P. (1982). Changes in water content and volume accompanying exsheathment in Haemonchus contortus. International Journal for Parasitology 12, 93–6.Google Scholar
Edney, E. B. (1977). Water Balance in Land Arthropods. Berlin: Springer-Verlag.Google Scholar
Ellenby, C. (1968). Determination of the water content of nematode worms by interference microscopy. Experientia 24, 84–5.Google Scholar
Ellenby, C. & Perry, R. N. (1976). The influence of the hatching factor in the water uptake of the second stage larva of the potato cyst nematode Heterodera rostochiensis. Journal of Experimental Biology 64, 141–7.Google Scholar
Fetterer, R. H. (1986). Transcuticular solute movement in parasitic nematodes: relationship between non-polar solute transport and partition coefficient. Comparative Biochemistry and Physiology 83A, 461–6.Google Scholar
Gilby, A. R. (1980). Transpiration, temperature and lipids in insect cuticle. Advances in Insect Physiology 15, 1–33.Google Scholar
Greenaway, P. (1970). Sodium regulation in the freshwater mollusc Limnea stagnalis (L) (Gastropoda, Pulmonata). Journal of Experimental Biology 53, 147–63.Google Scholar
Grover, D. W. & Nicol, J. M. (1940). The vapour pressure of glycerin solutions at 20°. Journal of the Society of Chemical Industry 59, 175–7.Google Scholar
Kennedy, M. W., Foley, M., Kuo, Y. -M., Kusel, J. R. & Garland, P. B. (1987). Biophysical properties of the surface lipid of parasitic nematodes. Molecular and Biochemical Parasitology 22, 233–40.Google Scholar
Marks, C. F., Thomason, I. J. & Castro, C. E. (1968). Dynamics of the penetration of nematodes by water, nematocides and other substances. Experimental Parasitology 22, 321–37.CrossRefGoogle Scholar
Marshall, E. & Howells, R. E. (1986). Turnover of the surface proteins of adult and third and fourth stage larval Brugia pahangi. Molecular and Biochemical Parasitology 18, 17–24.CrossRefGoogle ScholarPubMed
Pasternak, J. & Leushner, J. R. A. (1975). Programmed collagen synthesis during postembryonic development of the nematode Panagrellus silusiae: effect of transcription and translation inhibitors. Journal of Experimental Zoology 194, 519–28.Google Scholar
Perry, R. N. (1977 a). The water dynamics of stages of Ditylenchus dipsaci and D. myceliophagus during desiccation and rehydration. Parasitology 75, 45–70.Google Scholar
Perry, R. N. (1977 b). The effect of previous desiccation on the ability of 4th-stage larvae of Ditylenchus dipsaci to control rate of water loss and to survive desiccation. Parasitology 75, 215–31.CrossRefGoogle Scholar
Preston, C. M. & Bird, A. F. (1987). Physiological and morphological changes associated with recovery from anabiosis in the dauer larva of the nematode Anguina agrostis. Parasitology 95, 125–33.Google Scholar
Reisin, I. C. & de Falla, P. (1984). Membrane permeability of secondary hydatid cysts of Echinococcus granulosus. Determination of the water diffusional and osmotic permeability coefficients through a syncytial membrane. Molecular and Biochemical Parasitology 12, 101–16.CrossRefGoogle ScholarPubMed
Searcy, D. G., Kisiel, M. J. & Zuckerman, B. M. (1976). Age-related increase of cuticle permeability in the nematode Caenorhabditis briggsae. Experimental Aging Research 2, 293–301.CrossRefGoogle ScholarPubMed
Webb, J. L. (1963). Enzyme and Metabolic Inhibitors. New York and London: Academic Press.Google Scholar
Wharton, D. A. (1986). A Functional Biology of Nematodes. London and Sydney: Croom Helm.Google Scholar
Wharton, D. A. & Barrett, J. (1985). Ultrastructural changes during recovery from anabiosis in the plant nematode, Ditylenchus. Tissue and Cell 17, 79–96.Google Scholar
Wharton, D. A., Barrett, J. & Perry, R. N. (1985). Water uptake and morphological changes during recovery from anabiosis in the plant parasitic nematode, Ditylenchus dipsaci. Journal of Zoology (London) 206, 391–402.Google Scholar
Womersley, C. (1981). The effect of dehydration and rehydration on salt loss in the second-stage larvae of Anguina tritici. Parasitology 82, 411–19.CrossRefGoogle Scholar
Wright, D. J. & Newall, D. R. (1980). Osmotic and ionic regulation in nematodes. In Nematodes as Biological Models, vol. 2 (ed. Zuckerman, B. M.). New York and London: Academic Press.Google Scholar