Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-19T22:59:01.420Z Has data issue: false hasContentIssue false
  • English
  • Français

Carbohydrate reserves and infectivity in Trichinella spiralis isolated from carrion

Published online by Cambridge University Press:  06 April 2009

Karen M. Raines  and
G. L. Stewart
Karen M. Raines
Affiliation:
Center for Parasitology, Department of Biology, The University of Texas at Arlington, Arlington, Texas 76019
G. L. Stewart
Affiliation:
Center for Parasitology, Department of Biology, The University of Texas at Arlington, Arlington, Texas 76019
Get access Rights & Permissions [Opens in a new window]

Summary

Trichinous mice were killed and their carcasses maintained at room temperature or subzero temperatures for varying lengths of time. Some worm parameters were measured after direct isolation from carcasses while others were measured following passage through a second round of hosts. Glycogen and trehalose levels in infective 1st-stage larvae (L1) isolated directly from carcasses maintained at room temperature were significantly less than controls (day 0) after day 4 post-kill (p.k.). By day 21 p.k. among L1 isolated directly from mouse carcasses those observed coiled or moving had decreased by around 20% compared to day 0 p.k. The percentage of L1 isolated from carcasses on several days p.k., used to infect mice and recovered as pre-adult worms declined significantly after they had remained in carcasses for 14 days. Only 2·6% of muscle larvae isolated from carcasses on day 21 p.k. and used to infect mice were recoverable as pre-adults. Pre-adult worms raised in mice infected with larvae from day 7 carcasses had about 50% less glycogen than worms raised in mice infected with larvae isolated from fresh carcasses (day 0 p.k.). The fecundity in vitro of adult worms isolated from mice on day 7 following infection with infective L1 larvae maintained in carcasses held at room temperature for 0–16 days declined only when adult worms developed from larvae recovered from carcasses at 3 days following host death. Following 24 h at temperatures below freezing, infectivity of L1 larvae isolated from frozen carcasses was reduced by 97%. Carbohydrate levels remained high in larvae from carcasses frozen for up to 4 days.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 3 , June 1988 , pp. 533 - 541
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCE

Barrientos, J., & Torres, P., (1982). Sobrevidado y capacidad infectante de Trichinella spiralis en el microambiente. Revues en Medicine de Chile 110, 1059–62.Google Scholar
Bendall, J. R., (1973). Postmortem changes in muscle. In The Structure and Function of Muscle (ed. Bourne, G. H.), pp. 243–309. New York: Academic Press.CrossRefGoogle Scholar
Boyd, E. M., & Huston, E. J., (1954). The distribution, longevity and sex ratio of Trichinella spiralis in hamsters following an initial infection. Journal of Parasitology 40, 686–90.CrossRefGoogle ScholarPubMed
Cameron, T. W. M., (1970). Trichinosis in Canada. In Trichinosis in Man and Animals (ed. Gould, S. E.), pp. 374–77. Springfield, Illinois: Charles C. Thomas.Google Scholar
Campbell, W. C., (1983). Epidemiology. I. Modes of Transmission. In Trichinella and Trichinosis (ed. Campbell, W. C.), pp. 425–44. New York: Plenum Press.CrossRefGoogle Scholar
Castro, G. A., & Fairbairn, D., (1969). Carbohydrates and lipids in Trichinella spiralis larvae and their utilization in vitro. Journal of Parasitology 55, 51–8.CrossRefGoogle ScholarPubMed
Denham, D. A., (1968). Immunity to Trichinella spiralis. III. The longevity of the intestinal phase of the infection in mice. Journal of Helminthology 62, 974–7.Google Scholar
Dennis, D., Despommier, D. D., & Davis, N., (1970). The infectivity of the newborn larva of Trichinella spiralis in the rat. Journal of Parasitology 56, 974–7.CrossRefGoogle ScholarPubMed
Despommier, D. D., (1983). Biology. In Trichinella and Trichinosis (ed. Campbell, W. C.), pp. 75–152. New York: Plenum Press.CrossRefGoogle Scholar
Despommier, D. D., Sukhedo, M., & Meerovitch, E., (1978). Trichinella spiralis: site selection by the larva during the enteral phase of the infection in mice. Experimental Parasitology 44, 209–15.CrossRefGoogle ScholarPubMed
Ferguson, J. D., & Castro, G. A., (1973). Metabolism of intestinal stages of Trichinella spiralis. American Journal of Physiology 225, 85–9.CrossRefGoogle ScholarPubMed
Kilgore, M. W., Stewart, G. L., & Lou, M., (1986). Chemical composition of newborn larvae, muscle larvae and adult Trichinella spiralis. International Journal for Parasitology 16, 455–60.CrossRefGoogle ScholarPubMed
Kim, C., (1983). Geographic distribution and prevalence. In Trichinella and Trichinosis (ed. Campbell, W. C.) pp. 445–500. New York: Plenum Press.CrossRefGoogle Scholar
Kozar, A., (1962). Incidence of Trichinella spiralis in the world and actual problems connected with trichinellosis. In Trichinellosis (ed. Kozar, Z.), pp. 15–67. Warsaw: Polish Scientific Publishers.Google ScholarPubMed
Kurylo-Borowski, Z., & Kozar, Z., (1960). The general chemical composition of muscle Trichinella spiralis larvae (abstract). Wiad Parazytologica 6, 357–9.Google Scholar
Larsh, J. E., & Kent, D. E., (1949). The effect of alcohol on natural and acquired immunity of mice to infection with Trichinella spiralis. Journal of Parasitology 35, 45–53.CrossRefGoogle ScholarPubMed
Madsen, H., (1976). The life cycle of Trichinella spiralis, with remarks on epidemiology and a new diagram. Acta Parasitologica Polonica 24, 143–58.Google Scholar
Montgomery, R., (1959). Determination of glycogen. Archives of Biochemistry and Biophysics 67, 378–86.CrossRefGoogle Scholar
Pawlowski, Z. S., (1981). Control of trichinellosis. In Trichinellosis (ed. Kim, C. W., Ruitenberg, E. J. and Teppema, J. S.), pp. 7–20. Chertsey, England: Reedbooks.Google Scholar
Podhajecky, K., (1963). Male—female ratio of Trichinella spiralis in various phases of infection in white mice. Biologica 18, 75–8.Google Scholar
Rausch, R. L., (1970). Trichinosis in the Arctic. In Trichinosis in Man and Animals (ed. Gould, S. E.), pp. 348–73. Springfield, Illinois: Charles C. Thomas.Google Scholar
Sokal, R. R., & Rohlf, F. J., (1980). Biometry, 2nd ed.New York: W. H. Freeman.Google Scholar
Steele, J. H., & Schultz, M. G., (1978). Trichinellosis: A review of the current problem. In Trichinellosis (ed. Kim, C. W. and Pawlowski, Z. S.), pp. 45–75. Hanover, New Hampshire: University Press of New England.Google Scholar
Stewart, G. L., Despommier, D. D., Burnham, J., & Raines, K. M., (1987). Trichinella spiralis: Behaviour, structure, and biochemistry of larvae following exposure to components of the host enteric environment. Experimental Parasitology 63, 195–204.CrossRefGoogle ScholarPubMed
Stewart, G. L., Raines, K. M., & Kilgore, M. W., (1986). Glucose absorption in vitro by the enteric stages of Trichinella spiralis. Parasitology 93, 581–6.CrossRefGoogle ScholarPubMed
Stewart, G. L., & Read, C. P., (1972). Ribonucleic acid metabolism in mouse trichinosis. Journal of Parasitology 58, 252–6.CrossRefGoogle ScholarPubMed
Stoinenov, K., & Gradinarski, I., (1981). On the epizootiology of Trichinella spiralis. In Trichinellosis, (ed. Kim, C. W., Ruitenberg, E. J. and Teppema, J. S.), pp. 373–6. Chertsey, England: Reedbooks.Google Scholar
Zimmerman, W. J., (1971). Trichinosis. In Parasitic Diseases of Wild Animals, (ed. Davis, J. W. and Anderson, R. C.), pp. 121–29. Ames, Iowa: Iowa State University Press.Google Scholar