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Capture and penetration processes of the free-living juveniles of Trichostrongylus colubriformis (Nematoda) by the nematophagous fungus, Arthrobotrys oligospora

Published online by Cambridge University Press:  06 April 2009

D. S. Murray  and
D. A. Wharton
D. S. Murray
Affiliation:
Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand
D. A. Wharton
Affiliation:
Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand
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Summary

The nematode-trapping fungus Arthrobotrys oligospora traps and invades all the free-living juvenile stages of the tri-chostrongyle nematode Trichostrongylus colubriformis. The processes of capture and invasion of the 1st-stage juveniles are described using differential interference contrast optics and 3-D reconstruction techniques. The adhesive responsible for capture is well preserved using a freeze-substitution technique for scanning electron microscopy. The invasion process of the ensheathed 3rd-stage juvenile of T. colubriformis takes much longer than in the 1st- or 2nd-stage juvenile and involves the formation of secondary infection pegs between the sheath and the cuticle which appear to penetrate the cuticle by physical pressure.

Keywords

Arthrobotrys oligosporaTrichostrongylus colubriformisnematophagous fungustrichostrongyle nematodebiological control3-D reconstructionscanning electron microscopy
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 93 - 100
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Dowsett, J. A. & Reid, J. (1979). Observations on the trapping of nematodes by Dactylaria scaphoides using optical, transmission and scanning electron microscope techniques. Mycologia 71, 379–91.CrossRefGoogle Scholar
Dowsett, J. A., Reid, J. & Hopkin, A. A. (1984). Microscopic observations on the trapping of nematodes by the predacious fungus Dactylaria cionopaga. Canadian Journal of Botany 62, 674–9.CrossRefGoogle Scholar
Dowsett, J. A., Reid, J. & Van Caeseele, L. (1977). Transmission and scanning electron microscope observations on the trapping of nematodes by Dactylaria brochopaga. Canadian Journal of Botany 55, 2945–55.CrossRefGoogle Scholar
Gray, N. F. (1987). Nematophagous fungi with particular reference to their ecology. Biological Reviews 62, 245–304.CrossRefGoogle Scholar
Gronvold, J., Korsholm, H., Wolstrup, J., Nansen, P. & Henriksen, S. A. (1985). Laboratory experiment to evaluate the ability of Arthrobotrys oligospora to destroy infective larvae of Cooperia species, and to investigate the effect of physical factors on the growth of the fungus. Journal of Helminthology 59, 119–25.CrossRefGoogle ScholarPubMed
Hashmi, H. A. & Connan, R. M. (1989). Biological control of ruminant trichostrongylids by Arthrobotrys oligospora, a predacious fungus. Parasitology Today 5, 28–30.CrossRefGoogle ScholarPubMed
Jansson, H. B., Jeyaprakash, A. & Zuckerman, B. M. (1985). Differential adhesion and infection of nematodes by the endoparasitic fungus Meria coniospora (Deuteromycetes). Applied and Environmental Microbiology 49, 552–5.CrossRefGoogle ScholarPubMed
Nansen, P., Gronvold, J., Henriksen, A. & Wolstrup, J. (1986). Predacious activity of the nematode-destroying fungus, Arthrobotrys oligospora, on preparasitic larvae of Cooperia oncophora and on soil nematodes. Proceedings of the Helminthological Society of Washington 53, 237–43.Google Scholar
Nordbring-Hertz, B. (1972). Scanning electron microscopy of the nematode-trapping organs of Arthrobotrys oligospora. Physiologia Plantarum 26, 279–84.Google Scholar
Nordbring-Hertz, B. (1977). X-ray microanalysis of the nematode-trapping organs in Arthrobotrys oligospora. Transactions of the British Mycological Society 68, 53–7.CrossRefGoogle Scholar
Nordbring-Hertz, B. & Stålhammar-Carlemalm, M. (1978). Capture of nematodes by Arthrobotrys oligospora, an electron microscope study. Canadian Journal of Botany 56, 1297–307.CrossRefGoogle Scholar
Shepherd, A. M. (1955). Formation of the infection bulb in Arthrobotrys oligospora Fresenius. Nature, London 175, 475.CrossRefGoogle Scholar
Veenhuis, M., Nordbring-Hertz, B. & Harder, W. (1985). An electron-microscopical analysis of capture and initial stages of penetration of nematodes by Arthrobotrys oligospora. Antonie van Leeuwenhoek 51, 385–98.CrossRefGoogle ScholarPubMed
Wharton, D. A. (1982). Observations on the coiled posture of trichostrongyle infective larvae using a freeze-substitution method and scanning electron microscopy. International Journal for Parasitology 12, 335–43.CrossRefGoogle ScholarPubMed
Wharton, D. A. (1986). A Functional Biology of Nematodes. London and Sydney: Croom Helm.CrossRefGoogle Scholar
Wharton, D. A. & Murray, D. S. (1989). 3-D reconstruction from optical sections of the nematode-trapping fungus, Arthrobotrys oligospora. Journal of Microscopy 154, 157–63.CrossRefGoogle Scholar
Wharton, D. A. & Murray, D. S. (1990 a). Preparation of the nematode-trapping fungus, Arthrobotrys oligospora, for scanning electron microscopy by freeze substitution. Journal of Microscopy.CrossRefGoogle ScholarPubMed
Wharton, D. A. & Murray, D. S. (1990 b). Carbohydrate/lectin interactions between the nematophagous fungus, Arthrobotrys oligospora, and the infective juveniles of Trichostrongylus colubriformis (Nematoda). Parasitology 101, 101–6.CrossRefGoogle ScholarPubMed