Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-07-07T20:19:27.416Z Has data issue: false hasContentIssue false
  • English
  • Français

Bloodstream Trypanosoma cruzi parasites from mice simultaneously express antigens that are markers of acute and chronic human Chagas disease

Published online by Cambridge University Press:  06 April 2009

M. S. Leguizamon ,
O. E. Campetella ,
M. B. Reyes ,
C. F. Ibañez ,
M. A. Basombrio ,
J. Rincon ,
A. Örn  and
A. C. C. Frasch
M. S. Leguizamon
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
O. E. Campetella
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
M. B. Reyes
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
C. F. Ibañez
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
M. A. Basombrio
Affiliation:
Laboratorio de Patología Experimental, Departamento de Ciencia de la Salud, Universidad Nacional de Salta, Calle Buenos Aires 177, 4400 Salta, Argentina
J. Rincon
Affiliation:
Department of Immunology, Karolinska Institute, Box 60.400, S-10401 Stockholm, Sweden
A. Örn
Affiliation:
Department of Immunology, Karolinska Institute, Box 60.400, S-10401 Stockholm, Sweden
A. C. C. Frasch
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
Get access Rights & Permissions [Opens in a new window]

Extract

Several recombinant Trypanosoma cruzi proteins previously isolated were used as antigens to analyse antibody specificities present in sera from human infections. Some parasite proteins such as SAPA (Shed Acute Phase Antigen) are antigenic early after infection. Others, like antigens 1 and 30, are antigenic mainly during the chronic phase of the infection. To understand why different proteins are antigenic at different periods of infection, specificities of antibodies present in the sera of infected mice were compared with the antigens expressed by parasites collected directly from blood. Parasites collected during the acute parasitaemia peak expressed not only antigen SAPA, but also antigens 1 and 30. However, only antibodies against SAPA were frequently observed during the early period and also in the chronic phase of murine infection. Long-lasting antibodies against SAPA were detected regardless of the mouse and parasite strains used. Furthermore, all 8 recombinant clones detected in a T. cruzi expression library with pooled sera from acutely infected mice were homologous to the SAPA gene. These results show that even though parasites from the acute parasitaemia peak in mice may express simultaneously several proteins known to be antigenic, only antibodies against SAPA were consistently detected.

Keywords

Trypanosoma cruziantigen markershumoral responserecombinant antigensantibodies
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 379 - 385
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Affranchino, J. L., Ibañez, C. F., Luquetti, A. O., Rassi, A., Reyes, M. B., Macina, R. A., Åslund, L., Pettersson, U. & Frasch, A. C. C. (1989). Identification of a Trypanosoma cruzi antigen that is shed during the acute phase of Chagas disease. Molecular and Biochemical Parasitology 34, 221–8.CrossRefGoogle ScholarPubMed
Araujo, F. G., Heilman, B. & Tighe, L. (1984). Antigens of Trypanosoma cruzi detected by different classes and subclasses of antibodies. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 672–7.CrossRefGoogle ScholarPubMed
Basombrio, M. A., Besuschio, S. & Cossio, P. M. (1982). Side effects of immunization with live attenuated Trypanosoma cruzi in mice and rabbits. Infection and Immunity 36, 342–50.CrossRefGoogle Scholar
Camargo, M. & Funayama, T. (1979). Diagnostico de labotario In Trypanosoma cruzi e doença de Chagas. (ed. Brener, Z. & Andrade, Z.) pp. 175–198. Brazil: Guanabara Koogan.Google Scholar
Dragon, E. A., Sias, S. R., Kato, E. A. & Gabe, J. D. (1987). The genome of Trypanosoma cruzi contains a constitutively expressed, tandemly arranged multicopy gene homologous to a major heat shock protein. Molecular and Cellular Biology 7, 1271–5.Google ScholarPubMed
Frasch, A. C. C. & Reyes, M. B. (1990). Diagnosis of Chagas disease. New approaches using recombinant DNA technology. Parasitology Today 6, 137–40.CrossRefGoogle Scholar
Gonzalez Cappa, S. M., Katzin, A. M., Añasco, N. & Lajmanovich, S. (1981). Comparative studies on infectivity and surface carbohydrates of several strains of Trypanosoma cruzi. Medicina (Buenos Aires) 41, 549–55.Google ScholarPubMed
Hoft, D. F., Kim, K. S., Otsu, K., Moser, D. R., Yost, W. J., Blumin, J. H., Donelson, J. E. & Kirchoff, L. V. (1989). Trypanosoma cruzi expresses diverse repetitive protein antigens. Infection and Immunity 57, 1959–67.CrossRefGoogle ScholarPubMed
Ibañez, C. F., Affranchino, J. L. & Frasch, A. C. C. (1987). Antigenic determinants of Trypanosoma cruzi defined by cloning of parasite DNA. Molecular and Biochemical Parasitology 25, 175–84.CrossRefGoogle ScholarPubMed
Ibañez, C. F., Affranchino, J. L., Macina, R. A., Reyes, M. B., Leguizamon, S., Camargo, M. E., Åslund, L., Pettersson, U. & Frasch, A. C. C. (1988). Multiple Trypanosoma cruzi antigens containing tandemly repeated amino acid sequence motifs. Molecular and Biochemical Parasitology 30, 27–34.CrossRefGoogle ScholarPubMed
Israelski, D. M., Sadler, R. & Araujo, F. G. (1988). Antibody response and antigen recognition in human infection with Trypanosoma cruzi. Aerican Journal of Tropical Medicine and Hygiene 39, 445–55.CrossRefGoogle ScholarPubMed
Kemp, D. J., Coppel, R. L. & Anders, R. F. (1987). Repetitive proteins and genes of malaria. Annual Review of Microbiology 41, 181–208.CrossRefGoogle ScholarPubMed
Krettli, A. U. (1982). Antibodies to Trypanosoma cruzi in experimental and human infections. African Journal of Clinical and Experimental Immunology 3, 327–45.Google Scholar
Krettli, A. U. & Brener, Z. (1982). Resistance against Trypanosoma cruzi associated to anti-living trypomastigotes antibodies. Journal of Immunology 128, 2009–12.CrossRefGoogle ScholarPubMed
Lafaille, J. J., Linss, J., Krieger, M. A., Souto-Padron, T., De Souza, W. & Goldenberg, S. (1989). Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes. Molecular and Biochemical Parasitology 35, 127–36.CrossRefGoogle ScholarPubMed
Levin, M. J., Mesri, E., Benarous, R., Levitus, G., Schijman, A., Levy-Yeyati, P., Chiale, P. A., Ruiz, A. M., Khan, A., Rosembaum, M. B., Torres, H. N. & Segura, E. L. (1989). Identification of major Trypanosoma cruzi antigenic determinants in chronic Chagas heart disease. American Journal of Tropical Medicine and Hygiene 41, 530–8.CrossRefGoogle ScholarPubMed
Peterson, D., Fouts, D. L. & Manning, J. E. (1989). The 85-kd surface antigen gene of Trypanosoma cruzi is telomeric and a member of a multigene family. The EMBO Journal 8, 3911–16.CrossRefGoogle Scholar
Peterson, D. S., Wrightsman, R. A. & Manning, J. E. (1986). Cloning of a major surface antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat. Nature, London 322, 566–8.CrossRefGoogle Scholar
Petry, S. & Eisen, H. (1989). Chagas disease: a model for the study of autoimmune diseases. Parasitology Today 5, 111–16.CrossRefGoogle Scholar
Reyes, M. B., Lorca, M., Muñoz, P. & Frasch, A. C. C. (1990). Fetal IgG specificities against Trypanosoma cruzi antigens in infected newborns. Proceedings of the National Academy of Sciences, USA 87, 2846–50.CrossRefGoogle ScholarPubMed
Sonia Martins, M., Hudson, L., Krettli, A. U., Cancado, J. R. & Brener, Z. (1985). Human and mouse sera recognize the same polypeptide associated with immunological resistance in Trypanosoma cruzi infection. Clinical and Experimental Immunology 61, 343–50.Google Scholar
Souto-Padron, T., Reyes, M. B., Leguizamon, S., Campetella, O., Frasch, A. C. C. & De Souza, W. (1989). Trypanosoma cruzi proteins that are antigenic during the acute and chronic periods of the infection are located in defined parasite regions. European Journal of Cell Biology 50, 272–8.Google Scholar
Yoshida, N. (1986). Trypanosoma cruzi recognition of trypomastigote surface antigens by lytic antisera from mice resistant to acute infection. Experimental Parasitology 61, 184–91.CrossRefGoogle ScholarPubMed
Zweerink, H. J., Weston, H. D., Andersen, O. F., Garber, S. S. & Hayes, E. C. (1984). Immunity against infection with Trypanosoma cruzi in mice correlates with presence of antibodies against three typomastigotes polypeptides. Infection and Immunity 46, 826–30.CrossRefGoogle Scholar