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Bloodstream Trypanosoma cruzi parasites from mice simultaneously express antigens that are markers of acute and chronic human Chagas disease

Published online by Cambridge University Press:  06 April 2009

M. S. Leguizamon ,
O. E. Campetella ,
M. B. Reyes ,
C. F. Ibañez ,
M. A. Basombrio ,
J. Rincon ,
A. Örn  and
A. C. C. Frasch
M. S. Leguizamon
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
O. E. Campetella
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
M. B. Reyes
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
C. F. Ibañez
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
M. A. Basombrio
Affiliation:
Laboratorio de Patología Experimental, Departamento de Ciencia de la Salud, Universidad Nacional de Salta, Calle Buenos Aires 177, 4400 Salta, Argentina
J. Rincon
Affiliation:
Department of Immunology, Karolinska Institute, Box 60.400, S-10401 Stockholm, Sweden
A. Örn
Affiliation:
Department of Immunology, Karolinska Institute, Box 60.400, S-10401 Stockholm, Sweden
A. C. C. Frasch
Affiliation:
Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina
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Extract

Several recombinant Trypanosoma cruzi proteins previously isolated were used as antigens to analyse antibody specificities present in sera from human infections. Some parasite proteins such as SAPA (Shed Acute Phase Antigen) are antigenic early after infection. Others, like antigens 1 and 30, are antigenic mainly during the chronic phase of the infection. To understand why different proteins are antigenic at different periods of infection, specificities of antibodies present in the sera of infected mice were compared with the antigens expressed by parasites collected directly from blood. Parasites collected during the acute parasitaemia peak expressed not only antigen SAPA, but also antigens 1 and 30. However, only antibodies against SAPA were frequently observed during the early period and also in the chronic phase of murine infection. Long-lasting antibodies against SAPA were detected regardless of the mouse and parasite strains used. Furthermore, all 8 recombinant clones detected in a T. cruzi expression library with pooled sera from acutely infected mice were homologous to the SAPA gene. These results show that even though parasites from the acute parasitaemia peak in mice may express simultaneously several proteins known to be antigenic, only antibodies against SAPA were consistently detected.

Keywords

Trypanosoma cruziantigen markershumoral responserecombinant antigensantibodies
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 379 - 385
Copyright
Copyright © Cambridge University Press 1991

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