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Biochemical and ultrastructural investigation of the effect of Stelazine (trifluoperazine) on Hymenolepis diminuta (Cestoda)

Published online by Cambridge University Press:  06 April 2009

Jayne B. Hipkiss ,
A. Skinner  and
C. J. Branford White
Jayne B. Hipkiss
Affiliation:
Department of Biology, Oxford Polytechnic, Headington, Oxford OX3 0BP
A. Skinner
Affiliation:
Department of Electron Microscopy, John Radcliffe Hospital11, Headington, Oxford 0X3 9DU
C. J. Branford White
Affiliation:
Bath College of Higher Education, Newton Park, Newton St Loe, Bath BA 9BN
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Summary

The effects of the phenothiazine, Stelazine, on Hymenolepis diminuta were investigated. The cestode was incubated for 10 min at 37 °C with 1 mM trifluoperazine, in the presence and absence of Ca2+. Assay of brush border enzymes showed that drug treatment lowered the activities of alkaline phosphatase, Ca2+-ATP'ase, 5′-nucleotidase and type 1 phosphodiesterase. This occurred in parallel with a significant reduction in tegumental protein. Under these conditions gross changes in ultrastructural appearance and cellular organization were observed. There was a lack of ordered microtriches and the distal cytoplasm was absent. Glycogen granules were scattered throughout the cytoplasm within the subtegumental layer. The connective tissue also appeared to be in some disarray. The effects of Stelazine appeared to be dependent on time and were significantly increased when Ca2+ was included in the incubation medium. Incubation with the less hydrophobic phenothiazine trifluoperazine sulphoxide had minimal effect on the integrity of the cestode. The results reported here support the premise that certain phenothiazines may be considered as potential cestocidal agents.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 1 , February 1987 , pp. 135 - 149
Copyright
Copyright © Cambridge University Press 1987

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