Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-26T00:37:30.592Z Has data issue: false hasContentIssue false
  • English
  • Français

Biochemical and histochemical studies on the non-specific phosphomonoesterases of Fasciola gigantica Cobbold 1855

Published online by Cambridge University Press:  06 April 2009

Allan J. Probert ,
Madan Goil  and
R. K. Sharma
Allan J. Probert
Affiliation:
Department of Applied Zoology, University College of North Wales, Bangor
Madan Goil
Affiliation:
Department of Zoology, Bareilly College, U.P., India
R. K. Sharma
Affiliation:
Department of Zoology, Bareilly College, U.P., India
Get access Rights & Permissions [Opens in a new window]

Extract

Biochemical studies on the non-specific phosphomonoesterases of Fasciola hepatica have shown the presence of acid phosphomonoesterase active at pH 4·6 and two forms of alkaline phosphomonoesterases active at pH 7·6 and pH 9·6 respectively. Histochemical studies have located acid phosphomonoesterase in the walls of the intestinal caeca and to a lesser extent in the tegument. Alkaline phosphomonoesterase was found to occur mainly in the excretory ducts and to a lesser degree in the uterine wall. Glycogen was mainly concentrated in the yolk glands, parenchyma, mature eggs and the non-muscular regions of both oral and ventral suckers.

Dr A. J. Probert takes pleasure in recording the support of the British Council and the Indian University Grants Commission to enable him to study in India.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 2 , April 1972 , pp. 347 - 353
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arme, C. (1966). Histochemical and biochemical studies on some enzymes of Ligula intestinalis. (Cestoda: Pseudophyllidea). Journal of Parasitology 52, 63–8.CrossRefGoogle ScholarPubMed
Barry, D. H., Mawdesley, L. E. Thomas & Malone, J. C. (1968). Enzyme histochemistry of the adult liver fluke. Experimental Parasitology 23, 355–60.CrossRefGoogle ScholarPubMed
Brand, T. von (1952). Chemical Physiology of Endoparasitic Animals. New York: Academic Press.Google Scholar
Brand, T. von & Mercado, T. L. (1961). Histochemical glycogen studies of Fasciola hepatica. Journal of Parasitology 47, 459–61.CrossRefGoogle Scholar
Coil, W. E. (1958). Alkaline phosphatase in the trematode excretory system. Proceedings of the Helminthological Society of Washington 25, 137–8.Google Scholar
Danielli, J. F. (1952). Structural factors in cell permeability and secretion. Symposium of the Society for Experimental Biology. No. 6. Structural Aspects of Cell Physiology. Cambridge University Press.Google Scholar
Fiske, C. H. & Subba, Y. Row (1925). The colorimetric determination of phosphorus. Journal of Biological Chemistry 66, 375–400.CrossRefGoogle Scholar
Goil, M. M. (1966). Physiological studies on trematodes: phosphatase systems in Gastrothylax crumenifer. Parasitology 56, 105–10.CrossRefGoogle ScholarPubMed
Halton, D. W. (1967). Studies on phosphatase activity in Trematoda. Journal of Parasitology 53, 46–54.CrossRefGoogle ScholarPubMed
Kaplan, N. O., Cioth, M., Hamolsky, M. & Beiber, R. E. (1960). Molecular heterogeneity and evaluation of enzymes. Science 131, 392–7.CrossRefGoogle Scholar
Ma, L. (1964). Acid phosphatase in Clonorchis sinensis. Journal of Parasitology 50, 235–40.CrossRefGoogle ScholarPubMed
Pantelouris, E. M. (1967). Esterases of Fasciola hepatica L. Research in Veterinary Science 8, 157–9.CrossRefGoogle Scholar
Pearse, A. G. E. (1968). Histochemistry Theoretical and Applied, 3rd ed., vol. 1. London: J and A Churchill Ltd.Google Scholar
Pennoit-DeCooman, E. & van Grembergen, G. (1942). Vergelikend Onderzoek van het Fermentensystem bij Vrijlevende en parasitaire Plathelminthen, vol. 4, pp. 7–77. Verslagen en voorstellen van de K. Vlaamse Academie voor Wetenschappen, leheren en schone Kunsten van België, Brussels.Google Scholar
Probert, A. J. (1966). Histochemical studies on the rediae and cercariae of Echinoparyphium recurvatum Linstow. Nature 210, 550–1.CrossRefGoogle Scholar
Read, C. P. (1949). Preliminary studies on the intermediary metabolism of the cestode Hymenolepis diminuta. Journal of Parasitology. 35 (Abstr. 58), 26–27.Google Scholar
Read, C. P. (1966). Nutrition of intestinal helminths. In Biology of Parasites, (ed. Soulsby, E. J. L.). New York: Academic Press.Google Scholar
Ruyter, J. H. C. (1964). Studies on an improved lead phosphate technique for the demonstration of non-specific acid phosphatase in non-deparaffinized organ and tissue sections. Histochemie 3, 521–37.CrossRefGoogle Scholar
Thorpe, E. (1968). Comparative enzyme histochemistry of immature and mature stages of Fasciola hepatica. Experimental Parasitology 22, 150–9.CrossRefGoogle ScholarPubMed
Waitz, J. A. & Schardein, J. L. (1964). Histochemical studies of four Cyclophyllidean Cestodes. Journal of Parasitology 50, 271–7.CrossRefGoogle ScholarPubMed
Yamao, Y. & Saito, A. (1952). Histochemical studies on endoparasites. IV. Distribution of phosphomonoesterases in the tissues of the liver Fluke Fasicola hepatica L. Jikken Seibutsugaku Ho. (Bulletin of Experimental Biology 25, 153–8. English summary.)Google Scholar