Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T19:32:51.472Z Has data issue: false hasContentIssue false
  • English
  • Français

Artificially transformed schistosomula of Schistosoma mansoni: mechanism of acquisition of protection against antibody-mediated killing

Published online by Cambridge University Press:  06 April 2009

C. A. P. Tavares ,
M. N. Cordeiro ,
T. A. Mota-Santos  and
G. Gazzinelli
C. A. P. Tavares
Affiliation:
Departamento de Bioquímica-Imunologia, Institute de Ciências Biolóogicas, U.F.M.G., C.P. 2486, Belo Horizonte, M.G., Brasil
M. N. Cordeiro
Affiliation:
Departamento de Bioquímica-Imunologia, Institute de Ciências Biolóogicas, U.F.M.G., C.P. 2486, Belo Horizonte, M.G., Brasil
T. A. Mota-Santos
Affiliation:
Departamento de Bioquímica-Imunologia, Institute de Ciências Biolóogicas, U.F.M.G., C.P. 2486, Belo Horizonte, M.G., Brasil
G. Gazzinelli
Affiliation:
Departamento de Bioquímica-Imunologia, Institute de Ciências Biolóogicas, U.F.M.G., C.P. 2486, Belo Horizonte, M.G., Brasil
Get access Rights & Permissions [Opens in a new window]

Summary

Incorporation of labelled amino acid into tegumental proteins and acquisition of protection by schistosomula against antibody-mediated killing in vitro were simultaneously stimulated by serum factors and inhibited by puromycin. Comparison of polyacrylamide gel electrophoresis patterns with fluorographic autoradiography indicates that the majority of proteins in the parasite tegument were labelled with the isotope after incubation for 3 h. No new, clearly defined band was observed in the autoradiography pattern. During this period a decreasing susceptibility of the schistosomula to antibody plus complement was observed. Quantitative fluorescence assay shows that schistosomula insensitive to antibody plus complement were still able to bind the same amount of antibody as the unprotected parasites. Pre-culture of schistosomula in the presence of inactivated normal rabbit serum also decreased the susceptibility of the parasites to the in vivo killing mechanism.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 95 - 104
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bonner, W. M. & Laskey, R. A. (1974). A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46, 83–8.CrossRefGoogle ScholarPubMed
Brink, L. H., McLaren, D. J. & Smithers, S. R. (1977). Schislosoma mansoni: a comparative study of artificially transformed schistosomula and schistosomula recovered after cercarial penetration of isolated skin. Parasitology 74, 73–86.CrossRefGoogle ScholarPubMed
Capron, A., Biguet, J., Vernes, A. & Afchain, D. (1968). Les structure antigenique des helminthes. Aspects immunologiques des relations hote–parasite. Pathologie et biologie, Paris 16, 121–38.Google Scholar
Clegg, J. A. (1974). Host antigens and the immune response in schistosomiasis. In Parasites in the Immunized Host: Mechanisms of Survival. Ciba Fdn Symp. 25 N.S., pp. 161–83. Amsterdam: Associated Scientific Publishers.Google Scholar
Clegg, J. A., & Smithers, S. R. (1972) The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–88.CrossRefGoogle ScholarPubMed
Damian, R. T. (1964). Molecular mimicry: antigen sharing by parasite and host and its consequences. American Naturalist 98, 129–49.CrossRefGoogle Scholar
Damian, R. T., Greene, N. D. & Hubbard, W. J. (1973). Occurrence of mouse α-2 macroglobulin antigenic determinants on Schistosoma mansoni adults with evidence of their nature. Journal of Parasitology 59, 64–73.CrossRefGoogle Scholar
Dean, D. A. & Sell, K. W. (1972). Surface antigens on Schistosoma mansoni. II. Adsorption of a Forssman-like host antigen by schistosomula. Clinical and Experimental Immunology 15, 525–40.Google Scholar
Dean, D. A. (1977). Decreased binding of cytotoxic antibody by developing Schistosoma mansoni. Evidence for surface change independent of host antigen adsorption and membrane turnover. Journal of Parasitology 63, 418–26.CrossRefGoogle ScholarPubMed
Gazzinelli, G., Oliveira, C. C., Figueiredo, E. A., Pereira, L. H., Coelho, P. M. Z. & Pellegrino, J. (1973). Schistosoma mansoni: biochemical evidence for morphogenetic change from cercaria to schistosomula. Experimental Parasitology 34, 181–8.Google Scholar
Goldring, O. L., Clegg, J. A., Smithers, S. R. & Terry, R. J. (1976). Acquisition of human blood group antigens by Schistosoma mansoni. Clinical and Experimental Immunology 26, 181–7.Google ScholarPubMed
Goldring, O. L., Sher, A., Smithers, S. R. & McLaren, D. J. (1977). Host antigens and parasite antigen of murine Schiatosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 144–8.CrossRefGoogle ScholarPubMed
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worms. International Journal for Parasitology 3, 13–25.CrossRefGoogle Scholar
Imohiosen, E. A. E., Sher, A. & Von Lichtenberg, F. (1978). Early developmental changes of the schistosomula of Schistosoma mansoni in vitro and in mouse lung. Parasitology 76, 317–26.CrossRefGoogle ScholarPubMed
Kemp, W. M., Merrit, S. C., Bogucki, M. S., Rosier, J. G. & Seed, J. R. (1977). Evidence for adsorption of heterospecific host immunoglobulin on the tegument of Schistosoma mansoni. Journal of Immunology 119, 1849–54.CrossRefGoogle ScholarPubMed
Kusel, J. R. & Mackenzie, P. (1975 a). The measurement of the relative turnover rates of proteins of the surface membranes and other fractions of Schistosoma mansoni in culture. Parasitology 71, 261–73.CrossRefGoogle ScholarPubMed
Kusel, J. R., Sher, A., Perez, H., Clegg, J. A. & Smithers, S. R. (1975 b). The use of radioactive isotopes in the study of specific schistosome membrane antigens. In Isotopes and Radiation in Parasitology IV, pp. 127—43. Vienna: International Atomic Energy Agency.Google Scholar
Laskey, R. A. & Mills, A. D. (1975). Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56, 335–41.Google ScholarPubMed
von Lichtenberg, F., Sher, A. & McIntyre, S. (1977). A lung model of schistosomiasis immunity in mice. American Journal of Pathology 87, 105–24.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, B. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
McLaren, D. J., Clegg, J. & Smithers, B. S. (1975). Acquisition of host antigen by young Schistosoma mansoni in mice: correlation with failure to bind antibody in vitro. Parasitology 70, 67–75.CrossRefGoogle ScholarPubMed
McLaren, D. J., Hockley, D. J., Goldring, O. L. & Hammond, B. J. (1978). A freeze fracture study of the developing tegumental outer membrane of Schistosoma mansoni. Parasitology 76, 327–48.CrossRefGoogle ScholarPubMed
Nagai, Y., Gazzinelli, G., Moraes, G. W. G. & Pellegrino, J. (1977). Protein synthesis during cercaria–schistosomulum transformation and early development of the Schistosoma mansoni larvae. Comparative Biochemistry and Physiology 57B, 27–30.Google Scholar
Sell, K. W. & Dean, D. A. (1972). Surface antigens on Schistosoma mansoni. I. Demonstration of host antigen on schistosomula and adult worms using the mixed antiglobulin test. Clinical and Experimental Immunology 12, 315–24.Google ScholarPubMed
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigen in schistosomiasis. Proceedings of the Royal Society of London, B 171, 483–94.Google ScholarPubMed
Studier, F. W. (1973). Analysis of bacteriophage T7 early RNAs and protein on slab gels. Journal of Molecular Biology 79, 237–48.CrossRefGoogle ScholarPubMed
Tavares, C. A. P., Soares, R. C., Coelho, P. M. Z. & Gazzinelli, G. (1978). Schistosoma mansoni: evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro. Parasitology 77, 225–33.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1977). The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni. Parasitology 74, 61–71.CrossRefGoogle ScholarPubMed