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Antibody responses of rodents to a tegument membrane preparation from adult Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Shirley M. Roberts ,
C. Boot  and
R. A. Wilson
Shirley M. Roberts
Affiliation:
Department of Biology, University of York, York YO1 5DD
C. Boot
Affiliation:
Department of Biology, University of York, York YO1 5DD
R. A. Wilson*
Affiliation:
Department of Biology, University of York, York YO1 5DD
*
*Reprint requests to Dr R. A. Wilson.
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Summary

A preparation highly enriched in tegument membranes from adult worms has been used to probe rodent responses to schistosome surface antigens. All exposure regimes in mice stimulated levels of anti-tegument IgG, measurable by ELISA. Plateau levels of circulating antibody attained were similar after mixed-sex and male-only infections, and vaccination with tegument membranes. Female-only infections and exposure to irradiated cercariae stimulated lower levels of anti-tegument antibody. Responses in rats were similar, but occurred more rapidly, probably due to the large initial parasite exposure. Although both mice and rats could be stimulated to produce high levels of anti-tegument antibody by multiple vaccination, consistent protection against a challenge was not obtained. Paradoxically, rats and mice vaccinated with irradiated cercariae developed low levels of antibody to tegument membrane antigens but were highly resistant to challenge. It was concluded that, in the range of sera examined, antibody responses to schistosome tegument membranes did not correlate with the immune status of the host.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 3 , December 1988 , pp. 425 - 435
Copyright
Copyright © Cambridge University Press 1988

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