Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-23T20:32:25.811Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody is responsible for the passive transfer of immunity to mice from rabbits, rats or mice vaccinated with attenuated Schistosoma japonicum cercariae

Published online by Cambridge University Press:  06 April 2009

N. A. Moloney  and
G. Webbe
N. A. Moloney
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
G. Webbe
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
Get access Rights & Permissions [Opens in a new window]

Summary

Sera from rabbits, rats and mice multiply-vaccinated with attenuated cercariae of Schistosoma japonicum conferred high levels of resistance against challenge to naive recipient mice (up to 97, 64 and 60% respectively). Vaccinated rabbit and rat sera were given before challenge and vaccinated mouse serum 5 days after challenge. To show that the protective factors in these sera were antibodies, vaccinated rabbit and mouse sera were fractionated by protein A-Sepharose and the fractions precipitated by 50% ammonium sulphate. The protein A-Sepharose binding or non-binding fractions in vaccinated rabbit serum transferred approximately equal levels of significant resistance to mice, suggesting that both the IgG and non-IgG components of vaccinated rabbit serum are protective. The major part of the protective activity in vaccinated mouse serum was transferred to recipients by the protein A-Sepharose binding fraction, i.e. the IgG antibodies. Heat inactivation of sera at 56 °C for 3 h affected the protective capacity of vaccinated rat sera, but not that of vaccinated rabbit or mouse sera.

Keywords

Schistosoma japonicumantibodyimmunity
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 235 - 239
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bickle, Q. D., Andrews, B. J., Doenhoff, M. J., Ford, M. J. & Taylor, M. G. (1985). Resistance against Schistosoma mansoni induced by highly irradiated infections: studies on species specificity of immunization and attempts to transfer resistance. Parasitology 90, 301–12.CrossRefGoogle ScholarPubMed
Bickle, Q. D. & Ford, M. J. (1982). Studies on the surface antigenicity and susceptibility to antibody-dependent killing of developing schistosomula using sera from chronically infected mice and mice vaccinated with irradiated cercariae. Journal of Immunology 128, 2101–6.CrossRefGoogle ScholarPubMed
Ey, P. L., Prowse, S. J. & Jenkin, C. R (1978). Isolation of pure IgGI, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose Immunochemistry 15, 429–36.CrossRefGoogle Scholar
Ford, M. J., Bickle, Q. D., Taylor, M. G. & Andrews, B. J. (1984). Passive transfer of resistance and the site of immune-dependent elimination of the challenge infection in rats vaccinated with highly irradiated cercariae of Schistosoma mansoni. Parasitology 89, 461–82.CrossRefGoogle ScholarPubMed
Ford, M. J., Dissous, C., Pierce, R. J., Taylor, M. G., Bickle, Q. D. & Capron, A. (1987). The isotypes of antibody responsible for the 'late' passive transfer of immunity in rats vaccinated with highly irradiated cercariae. Parasitology 94, 509–22.CrossRefGoogle ScholarPubMed
Mangold, B. L. & Dean, D. A. (1986). Passive transfer with serum and IgG antibodies of irradiated cercariae induced resistance against Schistosoma mansoni in mice. Journal of Immunology 136, 2644–8.CrossRefGoogle ScholarPubMed
Moloney, N. A., Bickle, Q. D. & Webbe, G. (1985). The induction of specific immunity against Schistosoma japonicum by the exposure of mice to ultraviolet attenuated cercariae. Parasitology 90, 313–23.CrossRefGoogle ScholarPubMed
Moloney, N. A., Hinchcliffe, P. & Webbe, G. (1987 b). Passive transfer of resistance to mice with sera from rabbits, rats or mice vaccinated with ultraviolet-attenuated cercariae of Schistosoma japonicum. Parasitology 94, 497–508.CrossRefGoogle ScholarPubMed
Moloney, N. A., Webbe, C. & Hinchcliffe, P. (1987 a). The induction of species-specific immunity against Schistosoma japonicum by exposure of rats to ultraviolet attenuated cercariae. Parasitology 94, 49–54.CrossRefGoogle Scholar