Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-24T11:34:13.565Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody dependent cell-mediated cytotoxicity against Trypanosoma cruzi

Published online by Cambridge University Press:  06 April 2009

I. A. Abrahamsohn  and
W. D. Da Silva
I. A. Abrahamsohn
Affiliation:
Laboratório Especial de Imunobiologia, Instituto Butantan, S. Paulo, Brasil.
W. D. Da Silva
Affiliation:
Laboratório Especial de Imunobiologia, Instituto Butantan, S. Paulo, Brasil.
Get access Rights & Permissions [Opens in a new window]

Summary

This paper describes in vitro antibody dependent cytotoxicity against Trypanosoma cruzi epimastigotes by normal mouse splenic lymphocytes. Cytotoxicity was expressed as the percentage reduction in the number of motile parasites upon incubation with lymphocytes at 37 °C in a denned medium. Failure of the non-motile parasites to regain motility and their ensuring degeneration at 28 °C in liver infusion tryptose (LIT) medium confirmed loss of motility as a criterion of cytotoxicity. Incubation of T. cruzi at 37 °C for 18 h in a defined medium per se did not interfere with motility but was followed by a lag phase of the growth curve in LIT medium at 28 °C. The lag phase was prolonged for T. cruzi which had previously been incubated at 37 °C in the absence of cells.

Type
Research Article
Information
Parasitology , Volume 75 , Issue 3 , December 1977 , pp. 317 - 323
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, S. J. & Krassner, S. M. (1975). Axenic culture of Trypanosoma cruzi in a chemically-defined medium. Journal of Parasitology 61, 144–5.CrossRefGoogle Scholar
Camargo, M. E. (1966). Fluorescent antibody test for the serodiagnosis of American trypano-somiasis. Technical modification employing preserved forms of Trypanosoma cruzi in a slide test. Revista do Institute de Medicina tropical de São Paulo 8, 227–34.Google Scholar
Camargo, M. E., Hoshino, S. & Siquetra, G. R. V. (1973). Hemagglutination with preserved sensitized cells, a practical test for routine serological diagnosis of American trypanosomiasis. Revista do Institute de Medicina tropical de São Paulo 15, 81–5.Google Scholar
Deane, M. P. & Kirchner, E. (1963). Life-cycle of Trypanosoma conorhini. Influences of temperature and other factors on growth and morphogenesis. Journal of Protozoology 10, 391–400.CrossRefGoogle ScholarPubMed
Fernandes, J. F. & Castellani, O. (1966). Growth characteristics and chemical composition of Trypanosoma cruzi. Experimental Parasitology 18, 195–202.CrossRefGoogle Scholar
Greenberg, A. H., Hudson, L., Sheen, L. & Roitt, I.M. (1973). Antibody dependent cell-mediated cytotoxicity due to a ‘Null’ lymphoid cell. Nature, New Biology 242, 111–13.Google ScholarPubMed
Greenberg, A. H., Shen, L. & Medley, G. (1975). Characteristics of the effector cells mediating cytotoxicity against antibody coated target cells. I. Phagocytic and non-phagocytic effector cell activity against erythrocyte and tumour target cells in a [51Cr] release cytotoxicity assay and [125I] IUDER growth inhibition assay. Immunology 29, 719–30.Google Scholar
Krettli, A. V. & Brener, Z. (1976). Protective effects of specific antibodies in Trypanosoma cruzi infections. Journal of Immunology 116, 755–60.CrossRefGoogle ScholarPubMed
Mkwananzi, J. B., Franks, D. & Baker, J. R. (1976). Cytotoxicity of antibody-coated try-panosomes by normal human lymphoid cells. Nature, London 259, 403–4.CrossRefGoogle ScholarPubMed
Roberson, E. L. & Hanson, W. L. (1974). Transfer of immunity to T. cruzi. Transactions of the Royal Society of Tropical Medicine and Hygiene 68, 338.CrossRefGoogle ScholarPubMed