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Antibodies to synthetic peptides based on band 3 motifs react specifically with Plasmodium falciparum (humanmalaria)-infected erythrocytes and block cytoadherence

Published online by Cambridge University Press:  06 April 2009

I. Crandall  and
I. W. Sherman
I. Crandall
Affiliation:
University of California Riverside, Riverside, California 92521, USA
I. W. Sherman
Affiliation:
University of California Riverside, Riverside, California 92521, USA
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Summary

Rabbit polyclonal and mouse monoclonal antibodies (Mabs) prepared against synthetic peptides patterned on exofacialloops 3 (amino acids 546–555) and 7 (821–834) of the human anion transport protein band 3 inhibited the cytoadherence of Plasmodium falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Mabs directed against exofacial loop4 (amino acids 628–642) did not inhibit adherence to a significant degree. The murine Mabs recognized only P. falciparum- infected erythrocytes suggesting that the epitopes of loops 3, 4 and 7 are normally cryptic in uninfected erythrocytes.

Keywords

Plasmodium falciparumsynthetic peptideshuman erythrocyte anion transporter (band 3)cytoadherence
Type
Research Article
Information
Parasitology , Volume 108 , Issue 4 , May 1994 , pp. 389 - 396
Copyright
Copyright © Cambridge University Press 1994

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