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An essentially synthetic liquid medium for Entamoeba histolytica*

Published online by Cambridge University Press:  06 April 2009

Eder L. Hansen  and
Hamilton H. Anderson
Eder L. Hansen
Affiliation:
From the Division of Pharmacology and Experimental Therapeutics, University of California Medical School, San Francisco, California, U.S.A.
Hamilton H. Anderson
Affiliation:
From the Division of Pharmacology and Experimental Therapeutics, University of California Medical School, San Francisco, California, U.S.A.
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Extract

An essentially synthetic medium has been developed for the cultivation of Entamoeba histolytica in association with organism t. It consists of buffered saline solution containing trace minerals, twelve amino acids, ten synthetic vitamins of the B complex, nucleic acid, cholesterol and rice starch. Cultures have been carried continuously in this medium for a period of eleven months.

We are greatly indebted for materials used in this study to the following: Drs M. S. Dunn, J. J. Eiler, D. M. Greenberg, L. D. Greenberg, E. E. Howe, H. D. Lightbody, H. S. Loring, H. Molitor, E. D. Stewart and E. L. R. Stokstad.

The authors wish to acknowledge the valuable advice and material aid provided by Drs Gladys Emerson and Gilberto G. Villela during the initial phase of this study.

Type
Research Article
Information
Parasitology , Volume 39 , Issue 1-2 , July 1948 , pp. 69 - 72
Copyright
Copyright © Cambridge University Press 1948

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References

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