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An ELISA for the detection of pentastomid infections in the rat

Published online by Cambridge University Press:  06 April 2009

D. A. C. Jones  and
J. Riley
D. A. C. Jones
Affiliation:
Department of Biological Sciences, The University of Dundee, Dundee DD1 4HN, Scotland
J. Riley
Affiliation:
Department of Biological Sciences, The University of Dundee, Dundee DD1 4HN, Scotland
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Extract

A specific enzyme-linked immunosorbent assay, based on a 48 kDa metallo-proteinase isolated from the frontal glands of a porocephalid pentastomid (Porocephalus crotali) in rat intermediate hosts, is described. SDS–PAGE analysis of whole nymphal homogenate, followed by Western blotting and probing with immune rat serum, revealed that only 2 protein bands label consistently; these are the 48 kDa metallo-proteinase and a 150 kDa metallo-proteinase from the body fluid, but only the former labels strongly. Furthermore this response is detected earlier and persists much longer. These characteristics uniquely qualify the 48 kDa metallo-proteinase as a diagnostic antigen. We review the literature concerning the related porocephalid Linguatula serrata from the nasal cavities of domestic dogs, which can zoonotically infect man principally through the ingestion of eggs. We conclude that the high prevalences in dogs in certain areas, most notably the Middle East, must inevitably signal significant human infections. Our ELISA can be readily adapted for the serodiagnosis of linguatulosis and this should establish whether our prediction is true.

Keywords

pentastomidserodiagnosiszoonosisELISA
Type
Research Article
Information
Parasitology , Volume 103 , Issue 3 , December 1991 , pp. 331 - 337
Copyright
Copyright © Cambridge University Press 1991

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