Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-20T00:30:23.520Z Has data issue: false hasContentIssue false
  • English
  • Français

An association between schistosomes and contrapsin, a mouse serine protease inhibitor (serpin)

Published online by Cambridge University Press:  06 April 2009

J. Modha ,
Vaishu Parikh ,
J. Gauldie  and
M. J. Doenhoff
J. Modha
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Boad, St Albans AL4 0XQ
Vaishu Parikh
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Boad, St Albans AL4 0XQ
J. Gauldie
Affiliation:
Department of Pathology, McMaster University, Hamilton, Ontario, CanadaL8N 3Z5
M. J. Doenhoff
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Boad, St Albans AL4 0XQ
Get access Rights & Permissions [Opens in a new window]

Summary

An antigen in homogenates of adult Schistosoma mansoni worms grown in mice, and immunoprecipitated by polyspecific rabbit antiserum, has been identified as contrapsin, a mouse serine protease inhibitor (serpin). In the serum of some mice infected with S. mansoni contrapsin was found with altered immunoelectrophoretic characteristics when compared with contrapsin from uninfected mice.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 99 - 109
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Baumstark, J. S. (1967). Studies on the elastase-serum protein interaction. 1. Molecular identity of the inhibitors in human serum and direct demonstration of inhibitor-elastase complexes by zone and immunoelectrophoresis. Archives of Biochemistry and Biophysics 118, 619–30.Google Scholar
Bloom, B. R. (1979). Games parasites play: how parasites evade immune surveillance. Nature, London 279, 21–6.CrossRefGoogle ScholarPubMed
Carrell, R. & Travis, J. (1985). Alpha1-antitrypsin and the serpins: variation and countervariation. Trends in Biochemical Sciences 10, 20–4.CrossRefGoogle Scholar
Damian, R. T., Greene, N. D. & Hubbard, W. J. (1973). Occurrence of mouse alpha2-macroglobulin antigenic determinants on Schistosoma mansoni adults, with evidence on their nature. Journal of Parasitology 59, 67–73.Google Scholar
Doenhoff, M. J., Pearson, S., Dunne, D. W., Bickle, Q., Lucas, S., Bain, J., Musallam, R. & Hassounah, O. (1981). Immunologic control of hepatotoxicity and parasite egg excretion in Schistosoma mansoni infections: stage specificity of the reactivity of immune serum in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 41–53.Google Scholar
Dunne, D. W., Aonew, A. M., Modha, J. & Doenhoff, M. J. (1986). Schistosoma mansoni egg antigens: preparation of rabbit antisera with monospecific immunoprecipitating activity, and their use in antigen characterization. Parasite Immunology 8, 575–86.CrossRefGoogle ScholarPubMed
Dunne, D. W., Bain, J., Lillywhite, J. & Doenhoff, M. J. (1984). The stage-, strain- and speciesspecificity of a Schistosoma mansoni egg antigen fraction (CEF6) with serodiagnostic potential. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 460–70.Google Scholar
Gauldie, J., Lamontagne, L. R., Horsewood, P. & Jenkin, E. (1980). Immunohistochemical localization of alpha1-antitrypsin in normal mouse liver and pancreas. American Journal of Pathology 101, 723–36.Google Scholar
Goudie, R. B., Horne, C. H. W. & Wilkinson, P. C. (1966). A simple method for producing antibody specific to a single selected diffusive antigen. Lancet ii, 1224–5.CrossRefGoogle Scholar
Hill, R. E. & Hastie, N. D. (1987). Accelerated evolution in the reactive centre regions of serine protease inhibitors. Nature, London 326, 96–9.Google Scholar
Hill, R. E., Shaw, P. H., Barth, R. K. & Hastie, N. D. (1985 a). A genetic locus closely linked to a protease inhibitor gene complex controls the level of multiple RNA transcripts. Molecular and Cellular Biology 5, 2114–22.Google Scholar
Hill, R. E., Shaw, P. H., Boyd, P. A., Baumann, H. & Hastie, N. D. (1985 b). Plasma protease inhibitors in mouse and man: divergence within the reactive centre regions. Nature, London 311, 175–7.Google Scholar
Kemp, W. M., Damian, R. T. & Greene, N. D. (1976). Immunocytochemical localization of IgG on adult Schistosoma mansoni tegumental surfaces. Journal of Parasitology 62, 830–2.Google Scholar
Lammle, B. & Griffin, J. H. (1985). Formation of the fibrin clot: the balance of procoagulant and inhibitory factors. Clinics in Haemalology 14, 281–342.Google Scholar
Lamontagne, L. R., Gauldie, J., Befus, A. D., McAdam, K. P. W. J., Baltz, M. W. & Pepys, M. B. (1984). The acute phase response in parasite infection. Nippostrongylus brasiliensis in the mouse. Immunology 52, 733–41.Google Scholar
Ouaissi, M. A., Cornette, J. & Capron, A. (1984). Occurrence of fibronectin antigenic determinants on Schistosoma mansoni lung schistosomula and adult worms. Parasitology 88, 85–96.Google Scholar
Robertson, N. P. & Cain, G. D. (1985). Isolation and characterization of glycosaminoglycans from Schistosoma mansoni. Comparative Biochemistry and Physiology 82 B, 299–306.Google ScholarPubMed
Ruppel, A., Rother, U., Vongerichten, H. & Diesfeld, H. J. (1983). Schistosoma mansoni: complement activation in human and rodent sera by living parasites of various developmental stages. Parasilology 87, 75–86.Google Scholar
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology 55, 695–700.Google Scholar
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society of London, B 171, 483–94.Google ScholarPubMed
Stead, N., Kaplan, A. P. & Rosenberg, R. D. (1976). Inhibition of activated Factor XII by antithrombin-heparin cofactor. Journal of Biological Chemistry 251, 6481–8.Google Scholar
Studier, F. W. (1973). Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79, 237–48.Google Scholar
Takahara, H. & Sinohara, H. (1982). Mouse plasma trypsin inhibitors. Isolation and characterization of alpha1-antitrypsin and contrapsin, a novel trypsin inhibitor. Journal of Biological Chemistry 257, 2438–46.Google Scholar
Takahara, H. & Sinohara, H. (1983). Inhibitory spectrum of mouse contrapsin and alpha1-antitrypsin against serine proteases. Journal of Biochemistry 93, 1411–19.Google Scholar
Tsang, V. C. W. & Damian, R. T. (1977). Demonstration and mode of action of an inhibitor for activated Hageman factor (factor IXa) of intrinsic blood coagulation pathway from Schistosoma mansoni. Blood 49, 619–33.Google Scholar
Wieme, R. J. (1959). An improved technique for agar-gel electrophoresis on agar-gel microscopic slides. Clinica Chimica Acta 4, 317–21.Google Scholar